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The Salinity and phosphorus mesocosm experiment in freshwater sawgrass wetlands: Determining the trajectory and capacity of freshwater wetland ecosystems to recover carbon losses from saltwater intrusion (FCE LTER), Florida, USA from 2015 to 2018


At a Glance


(Zip file containing 16 files)

Authors: Dong Yoon Lee, Ben Wilson, Shelby Servais, Viviana Mazzei, John Kominoski
Time period: 2015-02-01 to 2018-06-01
Package id: knb-lter-fce.1226.2
Dataset id:

How to cite:
Lee, D., B. Wilson, S. Servais, V. Mazzei, J. Kominoski. 2019. The Salinity and phosphorus mesocosm experiment in freshwater sawgrass wetlands: Determining the trajectory and capacity of freshwater wetland ecosystems to recover carbon losses from saltwater intrusion (FCE LTER), Florida, USA from 2015 to 2018. Environmental Data Initiative. https://doi.org/10.6073/pasta/a269722318964f74cb5cabf87f0d3fb3. Dataset accessed 2020-08-08.

Geographic Coverage
This data set contains the following files.

Water quality data
Attribute table details     Download

Aboveground sawgrass biomass data
Attribute table details     Download

Ecosystem carbon flux during saltwater intrusion experiment
Attribute table details     Download

Ecosystem carbon flux study during freshwater restoration experiment
Attribute table details     Download

Sawgrass litter decomposition and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass litter
Attribute table details     Download

Sawgrass leaf elemental analysis data
Attribute table details     Download

Root elemental analysis data
Attribute table details     Download

Belowground_biomass_manipulation
Attribute table details     Download

Soil bulk density and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass peat
Attribute table details     Download

Sawgrass Aboveground Net Primary Production
Attribute table details     Download

Soil methane flux study during saltwater intrusion experiment
Attribute table details     Download

Soil CO2 flux study during saltwater intrusion experiment
Attribute table details     Download

Extracellular enzyme activity on root litter and bulk soil during saltwater intrusion experiment
Attribute table details     Download

Elemental analysis of periphyton cores
Attribute table details     Download

Periphyton Accumulation Rates
Attribute table details     Download

Relative Abundance of Diatoms on PeriphytonPlates
Attribute table details     Download



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Detailed Metadata


  • Dataset Abstract
    In experimental wetland mesocosms located at Florida Bay Interagency Science Center, Key Largo, Florida, researchers continuously added salinity (approximately 6.9 g salt d-1) and phosphorus ( approximately 0.5 mg P d-1) to Cladium jamaicense peat monoliths from February 2015 to February 2017 and quantified changes in carbon partitioning. Several studies, focusing on the functional roles of marsh, soil, periphyton and microbe in the sawgrass-peat ecosystem, summarized detailed methodology and results (Wilson et al. 2019; Servais et al. 2019; Mazzei et al. in press). Briefly, salinity was increased (~10 ppt) and phosphorus was added (0.45 mg P d-1) to simulate four treatment effects (n = 24 plots): i) freshwater and no-added phosphorus, ii) freshwater and added phosphorus, iii) saltwater and no-added phosphorus, and iv) saltwater and added phosphorus. Upon the termination of manipulation study (early February 2017), containers holding water and peat-sawgrass cores were drained, rinsed, and refilled with only freshwater without any added nutrient and salt. Then, we experimentally restored freshwater to previous treatment and control mesocosms from February 2017 to June 2018 to examine the capacity of wetland ecosystems to recover carbon losses from saltwater intrusion. Note that FCE1226_Water_quality.csv summarizes water quality during both the manipulation and restoration study; however, all other files in the Dataset Title section only summarize results from the restoration study. Detailed methodology is provided below.
  • Geographic Coverage
    Bounding Coordinates
    Florida Bay Interagency Science Center, Key Largo, Florida
    N: 25.08522, S: 25.08522, E: -80.45188, W: -80.45188

  • Attributes
    • Data Table:   Water quality data
      Attribute Name:
      Binnum
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-soil monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FW= freshwater
      FWP= freshwater and phosphorus
      SW= saltwater
      SWP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      N_umol_pw
      Attribute Label:
      Attribute Definition:
      Nitrate+Nitrite as N in porewater
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      NO3_umol_pw
      Attribute Label:
      Attribute Definition:
      Nitrate as NO3-N; NO3-N = N+N - NO2-N in porewater
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      NO2_umol_pw
      Attribute Label:
      Attribute Definition:
      Nitrite as N, Dissolved in porewater
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      NH4_umol_pw
      Attribute Label:
      Attribute Definition:
      Ammonium as N in porewater
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      SRP_umol_pw
      Attribute Label:
      Attribute Definition:
      Soluble Reactive Phosphorus in porewater
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      DOC_umol_pw
      Attribute Label:
      Attribute Definition:
      Dissolved organic carbon in porewater
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      N_umol_sw
      Attribute Label:
      Attribute Definition:
      Nitrate+Nitrite as N in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      NO3_umol_sw
      Attribute Label:
      Attribute Definition:
      Nitrate as NO3-N; NO3-N = N+N - NO2-N in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      NO2_umol_sw
      Attribute Label:
      Attribute Definition:
      Nitrite as N, Dissolved in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      NH4_umol_sw
      Attribute Label:
      Attribute Definition:
      Ammonium as N in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      SRP_umol_sw
      Attribute Label:
      Attribute Definition:
      Soluble Reactive Phosphorus in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      DOC_umol_sw
      Attribute Label:
      Attribute Definition:
      Dissolved organic carbon in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      TN_umol_sw
      Attribute Label:
      Attribute Definition:
      Nitrogen, Total in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      TP_umol_sw
      Attribute Label:
      Attribute Definition:
      Phosphorus, Total in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      TOC_umol_sw
      Attribute Label:
      Attribute Definition:
      Total Organic Carbon in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerLiter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      salinity_pw
      Attribute Label:
      Attribute Definition:
      Salinity in porewater
      Storage Type:
      float
      Measurement Scale:
      Units: practicalSalinityUnit
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      conductivity_pw
      Attribute Label:
      Attribute Definition:
      Conductivity in porewater
      Storage Type:
      float
      Measurement Scale:
      Units: siemenPerCentimeter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      pH_pw
      Attribute Label:
      Attribute Definition:
      pH in porewater
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      temp_pw
      Attribute Label:
      Attribute Definition:
      Temperature in porewater
      Storage Type:
      float
      Measurement Scale:
      Units: celsius
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      salinity_sw
      Attribute Label:
      Attribute Definition:
      Salinity in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: practicalSalinityUnit
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      conductivity_sw
      Attribute Label:
      Attribute Definition:
      Conductivity in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: siemenPerCentimeter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      pH_sw
      Attribute Label:
      Attribute Definition:
      pH in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      temp_sw
      Attribute Label:
      Attribute Definition:
      Temperature in surface water
      Storage Type:
      float
      Measurement Scale:
      Units: celsius
      Number Type: real
      Missing Value Code:
      NA (missing)


    • Data Table:   Aboveground sawgrass biomass data
      Attribute Name:
      Binnum
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-aboveground sawgrass monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FW= freshwater
      FWP= freshwater and phosphorus
      SW= saltwater
      SWP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Totalbiomass
      Attribute Label:
      Attribute Definition:
      Total aboveground sawgrass biomass dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: gramPerMeterSquare
      Number Type: real
      Missing Value Code:
      NA (Missing)


    • Data Table:   Ecosystem carbon flux during saltwater intrusion experiment
      Attribute Name:
      Binnum
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-soil monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FW= freshwater
      FWP= freshwater and phosphorus
      SW= saltwater
      SWP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Light
      Attribute Label:
      Attribute Definition:
      Light treatment; 0_full_Sun: no cover; 1_shade: 1 layer of mesh shade; 2_shade: 2 layers of mesh shade; 3_Dark: dark cover
      Storage Type:
      string
      Measurement Scale:
      0_full_Sun= no cover
      1_shade= 1 layer of shade mesh
      2_shade= 2 layers of shade mesh
      3_Dark= dark cover
      Missing Value Code:
       

      Attribute Name:
      NEP
      Attribute Label:
      Attribute Definition:
      Net Ecosystem Production
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerMeterSquaredPerMinute
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      GEP
      Attribute Label:
      Attribute Definition:
      Gross Ecosystem Production
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerMeterSquaredPerMinute
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      ER
      Attribute Label:
      Attribute Definition:
      Ecosystem Respiration
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerMeterSquaredPerMinute
      Number Type: real
      Missing Value Code:
      NA (missing)


    • Data Table:   Ecosystem carbon flux study during freshwater restoration experiment
      Attribute Name:
      Binnum
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-soil monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FWX= freshwater
      FWP= freshwater and phosphorus
      SWX= saltwater
      SWP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Light
      Attribute Label:
      Attribute Definition:
      Light treatment; 0_full_Sun: no cover; 1_shade: 1 layer of mesh shade; 2_shade: 2 layers of mesh shade; 3_Dark: dark cover
      Storage Type:
      string
      Measurement Scale:
      0_full_Sun= no cover
      1_shade= 1 layer of shade mesh
      2_shade= 2 layers of shade mesh
      3_Dark= dark cover
      Missing Value Code:
       

      Attribute Name:
      T_air
      Attribute Label:
      Attribute Definition:
      Temperature in air
      Storage Type:
      float
      Measurement Scale:
      Units: celsius
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      T_soil
      Attribute Label:
      Attribute Definition:
      Temperature in soil 5cm deep
      Storage Type:
      float
      Measurement Scale:
      Units: celsius
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      T_box_i
      Attribute Label:
      Attribute Definition:
      Temperature inside incubation chamber at the beginning of incubation (0_full_Sun treatment)
      Storage Type:
      float
      Measurement Scale:
      Units: celsius
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      T_box_f
      Attribute Label:
      Attribute Definition:
      Temperature inside incubation chamber at the end of incubation (3_Dark treatment)
      Storage Type:
      float
      Measurement Scale:
      Units: celsius
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      PAR
      Attribute Label:
      Attribute Definition:
      Photosynthetically active radiation measured on the top of incubation chamber
      Storage Type:
      float
      Measurement Scale:
      Units: microEinsteinPerMeterSquaredPerSecond
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      CO2_ppm
      Attribute Label:
      Attribute Definition:
      The slope of CO2 change over ~3 min incubation for each light level. When CO2_ppm < 0, CO2 was being uptaked (i.e., net photosynthesis)
      Storage Type:
      float
      Measurement Scale:
      Units: partsPerMillionPerMinute
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Atmpresure
      Attribute Label:
      Attribute Definition:
      Atmospheric pressure
      Storage Type:
      float
      Measurement Scale:
      Units: kilopascal
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      GEP_ppm
      Attribute Label:
      Attribute Definition:
      Gross ecosystem productivity calculated by subtracting CO2_ppm at 0, 1, and 2 light levels from CO2_ppm at the dark
      Storage Type:
      float
      Measurement Scale:
      Units: partsPerMillionPerMinute
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      GEP_g
      Attribute Label:
      Attribute Definition:
      Gross ecosystem photosynthesis; Coefficients: Chamber volume: 312.97 L, plot area: 0.2032 m2; Equation: [GEP = (GEP{ppm/min} x Chamber volume{L} x kPa / 101.325 atm) / (0.08206 {atm x L / mol / K} x (T + 273.15))]
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerMeterSquaredPerMinute
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      NEE_g
      Attribute Label:
      Attribute Definition:
      Net ecosystem exchange
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerMeterSquaredPerMinute
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Note
      Attribute Label:
      Attribute Definition:
      Notes about observations
      Storage Type:
      string
      Measurement Scale:
      Notes about observations
      Missing Value Code:
      NA (missing)


    • Data Table:   Sawgrass litter decomposition and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass litter
      Attribute Name:
      Bin_num
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-soil monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FW= freshwater
      FWP= freshwater and phosphorus
      SW= salt water
      SWP= salt water and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Rep
      Attribute Label:
      Attribute Definition:
      Replicate
      Storage Type:
      string
      Measurement Scale:
      A= Replicate A
      B= Replicate B
      Missing Value Code:
       

      Attribute Name:
      Type
      Attribute Label:
      Attribute Definition:
      Vegetation type
      Storage Type:
      string
      Measurement Scale:
      sawgrass= Vegetation type = sawgrass
      cellulose= Vegetation type = cellulose
      wood= Vegetation type = wood
      Missing Value Code:
       

      Attribute Name:
      InitDW
      Attribute Label:
      Attribute Definition:
      Initial dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: gram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      TotalDW
      Attribute Label:
      Attribute Definition:
      Total dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: gram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_P
      Attribute Label:
      Attribute Definition:
      Total_P
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_C
      Attribute Label:
      Attribute Definition:
      Total_C
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_N
      Attribute Label:
      Attribute Definition:
      Total_N
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_P_ug_g
      Attribute Label:
      Attribute Definition:
      Total_P_ug_g
      Storage Type:
      float
      Measurement Scale:
      Units: microgramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_C_mg_g
      Attribute Label:
      Attribute Definition:
      Total_C_mg_g
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_N_mg_g
      Attribute Label:
      Attribute Definition:
      Total_N_mg_g
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)


    • Data Table:   Sawgrass leaf elemental analysis data
      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Date of sample
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Bin_num
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-aboveground sawgrass monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FW= freshwater
      FWP= freshwater and phosphorus
      SW= saltwater
      SWP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Total_P_ug_g
      Attribute Label:
      Attribute Definition:
      Total P in aboveground sawgrass dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: microgramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_C_mg_g
      Attribute Label:
      Attribute Definition:
      Total C in aboveground sawgrass dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_N_mg_g
      Attribute Label:
      Attribute Definition:
      Total N in aboveground sawgrass dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_C
      Attribute Label:
      Attribute Definition:
      Percent P in aboveground sawgrass leaf
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_N
      Attribute Label:
      Attribute Definition:
      Percent C in aboveground sawgrass leaf
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_P
      Attribute Label:
      Attribute Definition:
      Percent N in aboveground sawgrass leaf
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)


    • Data Table:   Root elemental analysis data
      Attribute Name:
      Binnum
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-soil monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Fine_dry
      Attribute Label:
      Attribute Definition:
      Gram of fine roots
      Storage Type:
      float
      Measurement Scale:
      Units: gram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Coarse_dry
      Attribute Label:
      Attribute Definition:
      Gram of coarse roots
      Storage Type:
      float
      Measurement Scale:
      Units: gram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Volume
      Attribute Label:
      Attribute Definition:
      Sum of volumes (cm^3) in three vertical sections of soils
      Storage Type:
      float
      Measurement Scale:
      Units: centimeterCubed
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      W_D1
      Attribute Label:
      Attribute Definition:
      Top soil volume dimension (cm); Depth1: 0-7.5cm
      Storage Type:
      float
      Measurement Scale:
      Units: centimeter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      D_D1
      Attribute Label:
      Attribute Definition:
      Top soil volume dimension (cm)
      Storage Type:
      float
      Measurement Scale:
      Units: centimeter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      W_D2
      Attribute Label:
      Attribute Definition:
      Mid soil volume dimension (cm); Depth2: 7.5-15cm
      Storage Type:
      float
      Measurement Scale:
      Units: centimeter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      D_D2
      Attribute Label:
      Attribute Definition:
      Mid soil volume dimension (cm)
      Storage Type:
      float
      Measurement Scale:
      Units: centimeter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      W_D3
      Attribute Label:
      Attribute Definition:
      Bottom soil volume dimension (cm); Depth3: 15-25cm
      Storage Type:
      float
      Measurement Scale:
      Units: centimeter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      D_D3
      Attribute Label:
      Attribute Definition:
      Bottom soil volume dimension (cm)
      Storage Type:
      float
      Measurement Scale:
      Units: centimeter
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Fine_P_ug_g
      Attribute Label:
      Attribute Definition:
      Total Phosphorus in fine roots
      Storage Type:
      float
      Measurement Scale:
      Units: microgramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Coarse_P_ug_g
      Attribute Label:
      Attribute Definition:
      Total Phosphorus in coarse roots
      Storage Type:
      float
      Measurement Scale:
      Units: microgramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Fine_C_mg_g
      Attribute Label:
      Attribute Definition:
      Total Carbon in fine roots
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Coarse_C_mg_g
      Attribute Label:
      Attribute Definition:
      Total Carbon in coarse roots
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Fine_N_mg_g
      Attribute Label:
      Attribute Definition:
      Total Nitrogen in fine roots
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Coarse_N_mg_g
      Attribute Label:
      Attribute Definition:
      Total Nitrogen in coarse roots
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Fine_P_percent
      Attribute Label:
      Attribute Definition:
      Percent P in fine roots
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Coarse_P_percent
      Attribute Label:
      Attribute Definition:
      Percent P in coarse roots
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Fine_C_percent
      Attribute Label:
      Attribute Definition:
      Percent C in fine roots
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Coarse_C_percent
      Attribute Label:
      Attribute Definition:
      Percent C in coarse roots
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Fine_N_percent
      Attribute Label:
      Attribute Definition:
      Percent N in fine roots
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Coarse_N_percent
      Attribute Label:
      Attribute Definition:
      Percent N in coarse roots
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_P_ug_g
      Attribute Label:
      Attribute Definition:
      Total Phosphorus in roots
      Storage Type:
      float
      Measurement Scale:
      Units: microgramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_C_mg_g
      Attribute Label:
      Attribute Definition:
      Total Carbon in roots
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_N_mg_g
      Attribute Label:
      Attribute Definition:
      Total Nitrogen in roots
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_P
      Attribute Label:
      Attribute Definition:
      Percent P in roots
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_C
      Attribute Label:
      Attribute Definition:
      Percent C in roots
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      Total_N
      Attribute Label:
      Attribute Definition:
      Percent N in roots
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (missing)


    • Data Table:   Belowground_biomass_manipulation
      Attribute Name:
      Bin
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-aboveground sawgrass monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FW= freshwater
      FWP= freshwater and phosphorus
      SW= saltwater
      SWP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      0to7cm
      Attribute Label:
      Attribute Definition:
      Gram of fine roots in 0-7cm soil column taken at end of experiment
      Storage Type:
      float
      Measurement Scale:
      Units: gram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      7to15cm
      Attribute Label:
      Attribute Definition:
      Gram of fine roots in 7-15cm soil column taken at end of experiment
      Storage Type:
      float
      Measurement Scale:
      Units: gram
      Number Type: real
      Missing Value Code:
      NA (missing)

      Attribute Name:
      15to25cm
      Attribute Label:
      Attribute Definition:
      Gram of fine roots in 15-25cm soil column taken at end of experiment
      Storage Type:
      float
      Measurement Scale:
      Units: gram
      Number Type: real
      Missing Value Code:
      NA (missing)


    • Data Table:   Soil bulk density and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass peat
      Attribute Name:
      Bin_num
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-soil monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FW= freshwater
      FWP= freshwater and phosphorus
      SW= saltwater
      SWP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Depth
      Attribute Label:
      Attribute Definition:
      Depth (1: 0-7.5cm, 2: 7.5-15cm, 3: 15-25cm)
      Storage Type:
      float
      Measurement Scale:
      Units: centimeter
      Number Type: natural
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Volume
      Attribute Label:
      Attribute Definition:
      Volumes
      Storage Type:
      float
      Measurement Scale:
      Units: centimeterCubed
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Height
      Attribute Label:
      Attribute Definition:
      Height of soil sample
      Storage Type:
      float
      Measurement Scale:
      Units: centimeter
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Width
      Attribute Label:
      Attribute Definition:
      Width of soil sample
      Storage Type:
      float
      Measurement Scale:
      Units: centimeter
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Length
      Attribute Label:
      Attribute Definition:
      Length of soil sample
      Storage Type:
      float
      Measurement Scale:
      Units: centimeter
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Wet_wt
      Attribute Label:
      Attribute Definition:
      Wet weight
      Storage Type:
      float
      Measurement Scale:
      Units: kilogram
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Dry_wt
      Attribute Label:
      Attribute Definition:
      Dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: gram
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Total_P_ug_g
      Attribute Label:
      Attribute Definition:
      Percent P in soil
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Total_C_mg_g
      Attribute Label:
      Attribute Definition:
      Percent C in soil
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Total_N_mg_g
      Attribute Label:
      Attribute Definition:
      Percent N in soil
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Total_P
      Attribute Label:
      Attribute Definition:
      Total P in soil dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: microgramPerGram
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Total_C
      Attribute Label:
      Attribute Definition:
      Total C in soil dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Total_N
      Attribute Label:
      Attribute Definition:
      Total N in soil dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: milligramPerGram
      Number Type: real
      Missing Value Code:
      NA (Not collected)

      Attribute Name:
      Bulk_density
      Attribute Label:
      Attribute Definition:
      Bulk density taken at end of experiment
      Storage Type:
      float
      Measurement Scale:
      Units: gramPerCentimeterCubed
      Number Type: real
      Missing Value Code:
      NA (Not collected)


    • Data Table:   Sawgrass Aboveground Net Primary Production
      Attribute Name:
      binnum
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-soil monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FW= freshwater
      FWP= freshwater and phosphorus
      SW= saltwater
      SWP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      ANPP
      Attribute Label:
      Attribute Definition:
      Aboveground net primary production
      Storage Type:
      float
      Measurement Scale:
      Units: gramPerMeterSquaredPerYear
      Number Type: real
      Missing Value Code:
       


    • Data Table:   Soil methane flux study during saltwater intrusion experiment
      Attribute Name:
      binnum
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-soil monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FWX= freshwater
      FWP= freshwater and phosphorus
      SWX= saltwater
      SWP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      SoilCH4
      Attribute Label:
      Attribute Definition:
      Soil methane flux
      Storage Type:
      float
      Measurement Scale:
      Units: nanomolesPerMeterSquaredPerSecond
      Number Type: real
      Missing Value Code:
      NA (Not collected)


    • Data Table:   Soil CO2 flux study during saltwater intrusion experiment
      Attribute Name:
      binnum
      Attribute Label:
      Attribute Definition:
      Bin number (a container used for incubating sawgrass-soil monolith)
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      treatment
      Attribute Label:
      Attribute Definition:
      FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      FWX= freshwater
      FWP= freshwater and phosphorus
      SWX= saltwater
      SWP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      SoilCO2
      Attribute Label:
      Attribute Definition:
      Soil carbon dioxide flux
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerMeterSquaredPerSecond
      Number Type: real
      Missing Value Code:
      NA (Missing)


    • Data Table:   Extracellular enzyme activity on root litter and bulk soil during saltwater intrusion experiment
      Attribute Name:
      days
      Attribute Label:
      Attribute Definition:
      number of days since the start of the experiment when the enzymes were sampled
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: natural
      Missing Value Code:
       

      Attribute Name:
      nut_trt
      Attribute Label:
      Attribute Definition:
      treatement conditons (np - no phosphorus, p - with phosphorus)
      Storage Type:
      string
      Measurement Scale:
      np= no phosphorus
      p= with phosphorus
      Missing Value Code:
       

      Attribute Name:
      water_trt
      Attribute Label:
      Attribute Definition:
      treatment conditions (fresh and salt)
      Storage Type:
      string
      Measurement Scale:
      fresh= freshwater
      salt= saltwater
      Missing Value Code:
       

      Attribute Name:
      trt_label
      Attribute Label:
      Attribute Definition:
      treatment label (fresh.P, fhresh, salt.P, salt)
      Storage Type:
      string
      Measurement Scale:
      fresh= freshwater
      fresh.P= freshwater with P
      salt= saltwater
      salt.P= saltwater with P
      Missing Value Code:
       

      Attribute Name:
      phos
      Attribute Label:
      Attribute Definition:
      phosphatase activity (umol/g/h)
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerGramPerHour
      Number Type: real
      Missing Value Code:
      NA (Missing)

      Attribute Name:
      ars
      Attribute Label:
      Attribute Definition:
      arylsulfatase activity (umol/g/h)
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerGramPerHour
      Number Type: real
      Missing Value Code:
      NA (Missing)

      Attribute Name:
      bg
      Attribute Label:
      Attribute Definition:
      beta-1,4-glucosidsase activity (umol/g/h)
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerGramPerHour
      Number Type: real
      Missing Value Code:
      NA (Missing)

      Attribute Name:
      cel
      Attribute Label:
      Attribute Definition:
      beta-1,4-cellobiosidase activity (umol/g/h)
      Storage Type:
      float
      Measurement Scale:
      Units: micromolesPerGramPerHour
      Number Type: real
      Missing Value Code:
      NA (Missing)


    • Data Table:   Elemental analysis of periphyton cores
      Attribute Name:
      Sampling_Event
      Attribute Label:
      Attribute Definition:
      Sampling period
      Storage Type:
      string
      Measurement Scale:
      Sampling period
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Periphyton sample collection date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Mesocosm
      Attribute Label:
      Attribute Definition:
      Mesocosm identification number
      Storage Type:
      string
      Measurement Scale:
      Mesocosm identification number
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      F: freshwater, FP: freshwater and phosphorus, S: saltwater, SP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      F= freshwater
      FP= freshwater and phosphorus
      S= saltwater
      SP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      TP
      Attribute Label:
      Attribute Definition:
      Periphyton total phosphorus concentration in micrograms per gram of periphyton
      Storage Type:
      float
      Measurement Scale:
      Units: microgramPerGram
      Number Type: real
      Missing Value Code:
      NA (data not collected)

      Attribute Name:
      TN
      Attribute Label:
      Attribute Definition:
      Periphyton total nitrogen concentration in micrograms per gram of periphyton
      Storage Type:
      float
      Measurement Scale:
      Units: microgramPerGram
      Number Type: real
      Missing Value Code:
      NA (data not collected)

      Attribute Name:
      TC
      Attribute Label:
      Attribute Definition:
      Periphyton total carbon concentration in micrograms per gram of periphyton
      Storage Type:
      float
      Measurement Scale:
      Units: microgramPerGram
      Number Type: real
      Missing Value Code:
      NA (data not collected)

      Attribute Name:
      Mineral_Content
      Attribute Label:
      Attribute Definition:
      Periphyton percent inorganic weight of total dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (data not collected)

      Attribute Name:
      Organic_Content
      Attribute Label:
      Attribute Definition:
      Periphyton percent organic weight of total dry weight
      Storage Type:
      float
      Measurement Scale:
      Units: percent
      Number Type: real
      Missing Value Code:
      NA (data not collected)

      Attribute Name:
      Chla
      Attribute Label:
      Attribute Definition:
      Periphyton chlorophyll-a concentration in micrograms per unit of ash-free dry mass
      Storage Type:
      float
      Measurement Scale:
      Units: microgramPerGram
      Number Type: real
      Missing Value Code:
      NA (data not collected)


    • Data Table:   Periphyton Accumulation Rates
      Attribute Name:
      Sampling_Event
      Attribute Label:
      Attribute Definition:
      Sampling period
      Storage Type:
      string
      Measurement Scale:
      Sampling period
      Missing Value Code:
       

      Attribute Name:
      Deploy_Date
      Attribute Label:
      Attribute Definition:
      Settlement plate deployment date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Retrieve_Date
      Attribute Label:
      Attribute Definition:
      Settlement plate retrieval data
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Mesocosm
      Attribute Label:
      Attribute Definition:
      Mesocosm identification number
      Storage Type:
      string
      Measurement Scale:
      Mesocosm identification number
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      F: freshwater, FP: freshwater and phosphorus, S: saltwater, SP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      F= freshwater
      FP= freshwater and phosphorus
      S= saltwater
      SP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Total_Mass_Accumulation_Rate
      Attribute Label:
      Attribute Definition:
      Periphyton total mass accumulation rate on settlement plates (clear acrylic) in milligrams per meter squared per day
      Storage Type:
      float
      Measurement Scale:
      Units: milligramsPerMeterSquaredPerDay
      Number Type: real
      Missing Value Code:
      NA (not collected)

      Attribute Name:
      Organic_Mass_Accumulation_Rate
      Attribute Label:
      Attribute Definition:
      Periphyton organic mass accumulation rate on settlement plates (clear acrylic) in milligrams per meter squared per day
      Storage Type:
      float
      Measurement Scale:
      Units: milligramsPerMeterSquaredPerDay
      Number Type: real
      Missing Value Code:
      NA (not collected)

      Attribute Name:
      Chla_Accumulation_Rate
      Attribute Label:
      Attribute Definition:
      Periphyton chlorophyll-a accumulation rate on settlement plates (clear acrylic) in micrograms per meter squared per day
      Storage Type:
      float
      Measurement Scale:
      Units: microgramsPerMeterSquaredPerDay
      Number Type: real
      Missing Value Code:
      NA (not collected)

      Attribute Name:
      Chla_Accumulation_Rate_AFDM
      Attribute Label:
      Attribute Definition:
      Periphyton chlorophyll-a accumulation rate on settlement plates (clear acrylic) in micrograms per gram of periphyton ash-free dry mass per day
      Storage Type:
      float
      Measurement Scale:
      Units: microgramsPerMeterSquaredPerDay
      Number Type: real
      Missing Value Code:
      NA (not collected)


    • Data Table:   Relative Abundance of Diatoms on PeriphytonPlates
      Attribute Name:
      Sampling_Event
      Attribute Label:
      Attribute Definition:
      sampling period
      Storage Type:
      string
      Measurement Scale:
      sampling period
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Attribute Definition:
      Sampling date
      Storage Type:
      date
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Mesocosm
      Attribute Label:
      Attribute Definition:
      Mesocosm identification number
      Storage Type:
      string
      Measurement Scale:
      Mesocosm identification number
      Missing Value Code:
       

      Attribute Name:
      Treatment
      Attribute Label:
      Attribute Definition:
      F: freshwater, FP: freshwater and phosphorus, S: saltwater, SP: saltwater and phosphorus
      Storage Type:
      string
      Measurement Scale:
      F= freshwater
      FP= freshwater and phosphorus
      S= saltwater
      SP= saltwater and phosphorus
      Missing Value Code:
       

      Attribute Name:
      Species
      Attribute Label:
      Attribute Definition:
      Diatom species
      Storage Type:
      string
      Measurement Scale:
      Diatom species
      Missing Value Code:
       

      Attribute Name:
      Abundance
      Attribute Label:
      Attribute Definition:
      relative abundance
      Storage Type:
      float
      Measurement Scale:
      Units: dimensionless
      Number Type: real
      Missing Value Code:
       


  • Methods
    Method Step

    Description
    Dataset:
    PeriphytonCores_Chemistry

    Ten mL periphyton mat subsamples were collected bimonthly, starting in April 2015, from each mesocosm using a plastic syringe. In the lab, the mat samples were placed in a 500-mL beaker and homogenized with deionized water (DI) water to a periphyton slurry with a minimum volume of 200-mL. The beaker was placed on a stir plate to facilitate continuous mixing while the slurry was subsampled for biomass, chl-a total carbon (TC), total nitrogen (TN), and total phosphorus (TP) analyses. The biomass and chl-a subsamples were analyzed using the same methods described for the settlement plate samples. The TP subsample was dried at 80 degrees C and pulverized with a mortar and pestle. We processed a known amount of each subsample and used colorimetric analysis to estimate TP concentration, expressed as micrograms gram AFDM, following the methods of Solorzano and Sharp (1980). TC and TN subsamples were ground down to a fine powder by mortar and pestle, dried at 60 degrees C, and analyzed for total C and N content using a Flash 1112 elemental analyzer (CE Elantech, Lakewood, New Jersey, USA) following standard procedures.
    PeriphytonPlates_AccumulationRates
    Clear, acrylic settlement plates (approximately 104 square centimeters) were deployed in each mesocosm to measure periphyton biomass and chlorophyll-a accumulation rates. The settlement plates were incubated on the soil surface of the mesocosm for two months to allow periphyton mats in the mesocosms to colonize the plates. At the end of the two-month incubation period, settlement plates were retrieved and transported to the laboratory, where they were scrapped clean using a razor blade and deionized water. Settlement plates were sterilized using a bleach solution and redeployed the following month. This was done quarterly from March 2015 to April 2016 for a total of 4 settlement plate samples per mesocosm over the duration of the experiment. The material scraped from the settlement plates was collected in a beaker and homogenized with deionized water (DI) water to a minimum volume of 200-mL. The beaker was placed on a stir plate to facilitate continuous mixing while the slurry was subsampled for biomass, chlorophyll-a, and diatom analysis. The biomass subsample was placed in a drying oven at 80 degrees C for at least 48 h to obtain a dry mass (g) measurement and then in a furnace where it was combusted at 500 degrees C for 1 h to obtain the ash (mineral) mass (g). We calculated ash-free dry mass (AFDM) by the loss-on-ignition method as the difference between the ash mass and the total dry mass. The chl-a subsample was filtered onto Whatman 25-mm diameter glass fiber filter (GFF) paper, frozen, and later subjected to 48 h 90 percent cold acetone extraction at 20 degrees C. Chl-a concentrations were determined using a Gildford FLUORO IV fluorometer (Gilford Instrument, Oberlin, Ohio, USA; excitation 435 nm; emission 667 nm). AFDM and chl-a accumulation rates (mgPerMeterSquaredPerDay) were calculated by standardizing AFDM and chl-a values by the area of the settlement plates and the number of days incubated.
    PeriphytonPlates_DiatomRelAbund
    A subsample of the periphyton material scrapped from the settlement plates was used to analyze diatom species composition. Diatom subsamples were cleaned of mineral debris and organic matter using the sulfuric acid oxidation methods prescribed by Hasle and Fryxell (1970). We pipetted a known volume of cleaned diatom sample onto a glass coverslip, permanently mounted it on a microscope slide with Naphrax (PhycoTech Inc., St. Joseph, Michigan, USA) mounting medium, and viewed the diatoms under a compound light microscope (Axioskop 2; Zeiss, Thornwood, New York) equipped with differential interference contrast and a digital camera (Leica DFC425, Wetzlar, Germany). We counted at least 500 diatom valves along random transects at 600× magnification under oil immersion and identified them to the species level. Raw diatom counts were converted to relative abundance by standardizing the number of valves counted for each species by the total number of valves counted. We chose to analyze periphyton diatom species composition from the settlement plates rather than samples taken from the mesocosm soil surface because, as diatoms are counted and identified from cleaned (oxidized) samples, testing treatment effects on the diatom assemblage using established mats would have been confounded by the presence of dead diatom frustules; using settlement plates allowed us to better pick up diatom community response to treatments over time.


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    Intellectual Rights
    This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

  • Keywords
    FCE, FCE LTER, Florida Coastal Everglades LTER, peat collapse, sea level rise, phosphorus, seawater, marshes, carbon cycling, sea level
  • Data Table and Format
    Data Table:  Water quality data

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    Water quality data
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    Water quality data
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    Data Table:  Aboveground sawgrass biomass data

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    Aboveground sawgrass biomass data
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    Aboveground sawgrass biomass data
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    Data Table:  Ecosystem carbon flux during saltwater intrusion experiment

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    Ecosystem carbon flux during saltwater intrusion experiment
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    Ecosystem carbon flux during saltwater intrusion experiment
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    Data Table:  Ecosystem carbon flux study during freshwater restoration experiment

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    Ecosystem carbon flux study during freshwater restoration experiment
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    Ecosystem carbon flux study during freshwater restoration experiment
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    FCE1226_Gas_flux_restoration.csv
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    Data Table:  Sawgrass litter decomposition and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass litter

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    Sawgrass litter decomposition and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass litter
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    Sawgrass litter decomposition and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass litter
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    Data Table:  Sawgrass leaf elemental analysis data

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    Sawgrass leaf elemental analysis data
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    Data Table:  Root elemental analysis data

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    Root elemental analysis data
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    FCE1226_Root_biomass_element.csv
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    Data Table:  Belowground_biomass_manipulation

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    Belowground_biomass_manipulation
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    Belowground_biomass_manipulation
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    FCE1226_Belowground_biomass_manipulation.csv
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    Data Table:  Soil bulk density and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass peat

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    Soil bulk density and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass peat
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    Soil bulk density and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass peat
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    Data Table:  Sawgrass Aboveground Net Primary Production

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    Sawgrass Aboveground Net Primary Production
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    FCE1226_ANPP.csv
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    Number of Records:
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    Data Table:  Soil methane flux study during saltwater intrusion experiment

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    Soil methane flux study during saltwater intrusion experiment
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    Soil methane flux study during saltwater intrusion experiment
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    FCE1226_SoilCH4_R.csv
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    Data Table:  Soil CO2 flux study during saltwater intrusion experiment

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    Soil CO2 flux study during saltwater intrusion experiment
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    Soil CO2 flux study during saltwater intrusion experiment
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    FCE1226_SoilCO2_R.csv
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    Number of Records:
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    Data Table:  Extracellular enzyme activity on root litter and bulk soil during saltwater intrusion experiment

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    Extracellular enzyme activity on root litter and bulk soil during saltwater intrusion experiment
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    Extracellular enzyme activity on root litter and bulk soil during saltwater intrusion experiment
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    Data Table:  Elemental analysis of periphyton cores

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    Elemental analysis of periphyton cores
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    Elemental analysis of periphyton cores
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    FCE1226_PeriphytonCores_Chemistry.csv
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    Data Table:  Periphyton Accumulation Rates

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    Periphyton Accumulation Rates
    Entity Description:
    Periphyton Accumulation Rates
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    FCE1226_PeriphytonPlates_AccumulationRates.csv
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    Data Table:  Relative Abundance of Diatoms on PeriphytonPlates

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    Relative Abundance of Diatoms on PeriphytonPlates
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    Relative Abundance of Diatoms on PeriphytonPlates
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