Dataset title: The Salinity and phosphorus mesocosm experiment in freshwater sawgrass wetlands: Determining the trajectory and capacity of freshwater wetland ecosystems to recover carbon losses from saltwater intrusion (FCE LTER), Florida, USA from 2015 to 2018 Dataset ID: doi:10.6073/pasta/a269722318964f74cb5cabf87f0d3fb3 Dataset Creator Name: Dong Yoon Lee Organization: SFWMD Email: dlee@sfwmd.gov Name: Ben Wilson Organization: FIU Email: bwils034@fiu.edu Name: Shelby Servais Organization: FIU Email: sserv005.fiu.edu Name: Viviana Mazzei Organization: FIU Email: vmazz001@fiu.edu Name: John Kominoski Organization: FIU Email: jkominos@fiu.edu Dataset Abstract In experimental wetland mesocosms located at Florida Bay Interagency Science Center, Key Largo, Florida, researchers continuously added salinity (approximately 6.9 g salt d-1) and phosphorus ( approximately 0.5 mg P d-1) to Cladium jamaicense peat monoliths from February 2015 to February 2017 and quantified changes in carbon partitioning. Several studies, focusing on the functional roles of marsh, soil, periphyton and microbe in the sawgrass-peat ecosystem, summarized detailed methodology and results (Wilson et al. 2019; Servais et al. 2019; Mazzei et al. in press). Briefly, salinity was increased (~10 ppt) and phosphorus was added (0.45 mg P d-1) to simulate four treatment effects (n = 24 plots): i) freshwater and no-added phosphorus, ii) freshwater and added phosphorus, iii) saltwater and no-added phosphorus, and iv) saltwater and added phosphorus. Upon the termination of manipulation study (early February 2017), containers holding water and peat-sawgrass cores were drained, rinsed, and refilled with only freshwater without any added nutrient and salt. Then, we experimentally restored freshwater to previous treatment and control mesocosms from February 2017 to June 2018 to examine the capacity of wetland ecosystems to recover carbon losses from saltwater intrusion. Note that FCE1226_Water_quality.csv summarizes water quality during both the manipulation and restoration study; however, all other files in the Dataset Title section only summarize results from the restoration study. Detailed methodology is provided below. Geographic Coverage Bounding Coordinates Geographic description: Florida Bay Interagency Science Center, Key Largo, Florida West bounding coordinate: -80.45188 East bounding coordinate: -80.45188 North bounding coordinate: 25.08522 South bounding coordinate: 25.08522 Temporal Coverage Start Date: 2015-02-01 End Date: 2018-06-01 Data Table Entity Name: Water quality data Entity Description: Water quality data Object Name: FCE1226_Water_quality.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Binnum Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-soil monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FW= freshwater FWP= freshwater and phosphorus SW= saltwater SWP= saltwater and phosphorus Missing Value Code: Attribute Name: N_umol_pw Attribute Label: Attribute Definition: Nitrate+Nitrite as N in porewater Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: NO3_umol_pw Attribute Label: Attribute Definition: Nitrate as NO3-N; NO3-N = N+N - NO2-N in porewater Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: NO2_umol_pw Attribute Label: Attribute Definition: Nitrite as N, Dissolved in porewater Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: NH4_umol_pw Attribute Label: Attribute Definition: Ammonium as N in porewater Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: SRP_umol_pw Attribute Label: Attribute Definition: Soluble Reactive Phosphorus in porewater Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: DOC_umol_pw Attribute Label: Attribute Definition: Dissolved organic carbon in porewater Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: N_umol_sw Attribute Label: Attribute Definition: Nitrate+Nitrite as N in surface water Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: NO3_umol_sw Attribute Label: Attribute Definition: Nitrate as NO3-N; NO3-N = N+N - NO2-N in surface water Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: NO2_umol_sw Attribute Label: Attribute Definition: Nitrite as N, Dissolved in surface water Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: NH4_umol_sw Attribute Label: Attribute Definition: Ammonium as N in surface water Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: SRP_umol_sw Attribute Label: Attribute Definition: Soluble Reactive Phosphorus in surface water Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: DOC_umol_sw Attribute Label: Attribute Definition: Dissolved organic carbon in surface water Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: TN_umol_sw Attribute Label: Attribute Definition: Nitrogen, Total in surface water Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: TP_umol_sw Attribute Label: Attribute Definition: Phosphorus, Total in surface water Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: TOC_umol_sw Attribute Label: Attribute Definition: Total Organic Carbon in surface water Storage Type: float Measurement Scale: Units: micromolesPerLiter Number Type: real Missing Value Code: NA (missing) Attribute Name: salinity_pw Attribute Label: Attribute Definition: Salinity in porewater Storage Type: float Measurement Scale: Units: practicalSalinityUnit Number Type: real Missing Value Code: NA (missing) Attribute Name: conductivity_pw Attribute Label: Attribute Definition: Conductivity in porewater Storage Type: float Measurement Scale: Units: siemenPerCentimeter Number Type: real Missing Value Code: NA (missing) Attribute Name: pH_pw Attribute Label: Attribute Definition: pH in porewater Storage Type: float Measurement Scale: Units: dimensionless Number Type: real Missing Value Code: NA (missing) Attribute Name: temp_pw Attribute Label: Attribute Definition: Temperature in porewater Storage Type: float Measurement Scale: Units: celsius Number Type: real Missing Value Code: NA (missing) Attribute Name: salinity_sw Attribute Label: Attribute Definition: Salinity in surface water Storage Type: float Measurement Scale: Units: practicalSalinityUnit Number Type: real Missing Value Code: NA (missing) Attribute Name: conductivity_sw Attribute Label: Attribute Definition: Conductivity in surface water Storage Type: float Measurement Scale: Units: siemenPerCentimeter Number Type: real Missing Value Code: NA (missing) Attribute Name: pH_sw Attribute Label: Attribute Definition: pH in surface water Storage Type: float Measurement Scale: Units: dimensionless Number Type: real Missing Value Code: NA (missing) Attribute Name: temp_sw Attribute Label: Attribute Definition: Temperature in surface water Storage Type: float Measurement Scale: Units: celsius Number Type: real Missing Value Code: NA (missing) Data Table Entity Name: Aboveground sawgrass biomass data Entity Description: Aboveground sawgrass biomass data Object Name: FCE1226_Aboveground_sawgrass_biomass.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Binnum Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-aboveground sawgrass monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FW= freshwater FWP= freshwater and phosphorus SW= saltwater SWP= saltwater and phosphorus Missing Value Code: Attribute Name: Totalbiomass Attribute Label: Attribute Definition: Total aboveground sawgrass biomass dry weight Storage Type: float Measurement Scale: Units: gramPerMeterSquare Number Type: real Missing Value Code: NA (Missing) Data Table Entity Name: Ecosystem carbon flux during saltwater intrusion experiment Entity Description: Ecosystem carbon flux during saltwater intrusion experiment Object Name: FCE1226_Gas_flux_manipulation.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Binnum Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-soil monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FW= freshwater FWP= freshwater and phosphorus SW= saltwater SWP= saltwater and phosphorus Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Light Attribute Label: Attribute Definition: Light treatment; 0_full_Sun: no cover; 1_shade: 1 layer of mesh shade; 2_shade: 2 layers of mesh shade; 3_Dark: dark cover Storage Type: string Measurement Scale: 0_full_Sun= no cover 1_shade= 1 layer of shade mesh 2_shade= 2 layers of shade mesh 3_Dark= dark cover Missing Value Code: Attribute Name: NEP Attribute Label: Attribute Definition: Net Ecosystem Production Storage Type: float Measurement Scale: Units: milligramPerMeterSquaredPerMinute Number Type: real Missing Value Code: NA (missing) Attribute Name: GEP Attribute Label: Attribute Definition: Gross Ecosystem Production Storage Type: float Measurement Scale: Units: milligramPerMeterSquaredPerMinute Number Type: real Missing Value Code: NA (missing) Attribute Name: ER Attribute Label: Attribute Definition: Ecosystem Respiration Storage Type: float Measurement Scale: Units: milligramPerMeterSquaredPerMinute Number Type: real Missing Value Code: NA (missing) Data Table Entity Name: Ecosystem carbon flux study during freshwater restoration experiment Entity Description: Ecosystem carbon flux study during freshwater restoration experiment Object Name: FCE1226_Gas_flux_restoration.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Binnum Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-soil monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FWX= freshwater FWP= freshwater and phosphorus SWX= saltwater SWP= saltwater and phosphorus Missing Value Code: Attribute Name: Light Attribute Label: Attribute Definition: Light treatment; 0_full_Sun: no cover; 1_shade: 1 layer of mesh shade; 2_shade: 2 layers of mesh shade; 3_Dark: dark cover Storage Type: string Measurement Scale: 0_full_Sun= no cover 1_shade= 1 layer of shade mesh 2_shade= 2 layers of shade mesh 3_Dark= dark cover Missing Value Code: Attribute Name: T_air Attribute Label: Attribute Definition: Temperature in air Storage Type: float Measurement Scale: Units: celsius Number Type: real Missing Value Code: NA (missing) Attribute Name: T_soil Attribute Label: Attribute Definition: Temperature in soil 5cm deep Storage Type: float Measurement Scale: Units: celsius Number Type: real Missing Value Code: NA (missing) Attribute Name: T_box_i Attribute Label: Attribute Definition: Temperature inside incubation chamber at the beginning of incubation (0_full_Sun treatment) Storage Type: float Measurement Scale: Units: celsius Number Type: real Missing Value Code: NA (missing) Attribute Name: T_box_f Attribute Label: Attribute Definition: Temperature inside incubation chamber at the end of incubation (3_Dark treatment) Storage Type: float Measurement Scale: Units: celsius Number Type: real Missing Value Code: NA (missing) Attribute Name: PAR Attribute Label: Attribute Definition: Photosynthetically active radiation measured on the top of incubation chamber Storage Type: float Measurement Scale: Units: microEinsteinPerMeterSquaredPerSecond Number Type: real Missing Value Code: NA (missing) Attribute Name: CO2_ppm Attribute Label: Attribute Definition: The slope of CO2 change over ~3 min incubation for each light level. When CO2_ppm < 0, CO2 was being uptaked (i.e., net photosynthesis) Storage Type: float Measurement Scale: Units: partsPerMillionPerMinute Number Type: real Missing Value Code: NA (missing) Attribute Name: Atmpresure Attribute Label: Attribute Definition: Atmospheric pressure Storage Type: float Measurement Scale: Units: kilopascal Number Type: real Missing Value Code: NA (missing) Attribute Name: GEP_ppm Attribute Label: Attribute Definition: Gross ecosystem productivity calculated by subtracting CO2_ppm at 0, 1, and 2 light levels from CO2_ppm at the dark Storage Type: float Measurement Scale: Units: partsPerMillionPerMinute Number Type: real Missing Value Code: NA (missing) Attribute Name: GEP_g Attribute Label: Attribute Definition: Gross ecosystem photosynthesis; Coefficients: Chamber volume: 312.97 L, plot area: 0.2032 m2; Equation: [GEP = (GEP{ppm/min} x Chamber volume{L} x kPa / 101.325 atm) / (0.08206 {atm x L / mol / K} x (T + 273.15))] Storage Type: float Measurement Scale: Units: milligramPerMeterSquaredPerMinute Number Type: real Missing Value Code: NA (missing) Attribute Name: NEE_g Attribute Label: Attribute Definition: Net ecosystem exchange Storage Type: float Measurement Scale: Units: milligramPerMeterSquaredPerMinute Number Type: real Missing Value Code: NA (missing) Attribute Name: Note Attribute Label: Attribute Definition: Notes about observations Storage Type: string Measurement Scale: Notes about observations Missing Value Code: NA (missing) Data Table Entity Name: Sawgrass litter decomposition and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass litter Entity Description: Sawgrass litter decomposition and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass litter Object Name: FCE1226_Litter_decomposition_element.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Bin_num Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-soil monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FW= freshwater FWP= freshwater and phosphorus SW= salt water SWP= salt water and phosphorus Missing Value Code: Attribute Name: Rep Attribute Label: Attribute Definition: Replicate Storage Type: string Measurement Scale: A= Replicate A B= Replicate B Missing Value Code: Attribute Name: Type Attribute Label: Attribute Definition: Vegetation type Storage Type: string Measurement Scale: sawgrass= Vegetation type = sawgrass cellulose= Vegetation type = cellulose wood= Vegetation type = wood Missing Value Code: Attribute Name: InitDW Attribute Label: Attribute Definition: Initial dry weight Storage Type: float Measurement Scale: Units: gram Number Type: real Missing Value Code: NA (missing) Attribute Name: TotalDW Attribute Label: Attribute Definition: Total dry weight Storage Type: float Measurement Scale: Units: gram Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_P Attribute Label: Attribute Definition: Total_P Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_C Attribute Label: Attribute Definition: Total_C Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_N Attribute Label: Attribute Definition: Total_N Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_P_ug_g Attribute Label: Attribute Definition: Total_P_ug_g Storage Type: float Measurement Scale: Units: microgramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_C_mg_g Attribute Label: Attribute Definition: Total_C_mg_g Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_N_mg_g Attribute Label: Attribute Definition: Total_N_mg_g Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (missing) Data Table Entity Name: Sawgrass leaf elemental analysis data Entity Description: Sawgrass leaf elemental analysis data Object Name: FCE1226_Sawgrass_leaf_element.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Date Attribute Label: Attribute Definition: Date of sample Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Bin_num Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-aboveground sawgrass monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FW= freshwater FWP= freshwater and phosphorus SW= saltwater SWP= saltwater and phosphorus Missing Value Code: Attribute Name: Total_P_ug_g Attribute Label: Attribute Definition: Total P in aboveground sawgrass dry weight Storage Type: float Measurement Scale: Units: microgramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_C_mg_g Attribute Label: Attribute Definition: Total C in aboveground sawgrass dry weight Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_N_mg_g Attribute Label: Attribute Definition: Total N in aboveground sawgrass dry weight Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_C Attribute Label: Attribute Definition: Percent P in aboveground sawgrass leaf Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_N Attribute Label: Attribute Definition: Percent C in aboveground sawgrass leaf Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_P Attribute Label: Attribute Definition: Percent N in aboveground sawgrass leaf Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Data Table Entity Name: Root elemental analysis data Entity Description: Root elemental analysis data Object Name: FCE1226_Root_biomass_element.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Binnum Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-soil monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Missing Value Code: Attribute Name: Fine_dry Attribute Label: Attribute Definition: Gram of fine roots Storage Type: float Measurement Scale: Units: gram Number Type: real Missing Value Code: NA (missing) Attribute Name: Coarse_dry Attribute Label: Attribute Definition: Gram of coarse roots Storage Type: float Measurement Scale: Units: gram Number Type: real Missing Value Code: NA (missing) Attribute Name: Volume Attribute Label: Attribute Definition: Sum of volumes (cm^3) in three vertical sections of soils Storage Type: float Measurement Scale: Units: centimeterCubed Number Type: real Missing Value Code: NA (missing) Attribute Name: W_D1 Attribute Label: Attribute Definition: Top soil volume dimension (cm); Depth1: 0-7.5cm Storage Type: float Measurement Scale: Units: centimeter Number Type: real Missing Value Code: NA (missing) Attribute Name: D_D1 Attribute Label: Attribute Definition: Top soil volume dimension (cm) Storage Type: float Measurement Scale: Units: centimeter Number Type: real Missing Value Code: NA (missing) Attribute Name: W_D2 Attribute Label: Attribute Definition: Mid soil volume dimension (cm); Depth2: 7.5-15cm Storage Type: float Measurement Scale: Units: centimeter Number Type: real Missing Value Code: NA (missing) Attribute Name: D_D2 Attribute Label: Attribute Definition: Mid soil volume dimension (cm) Storage Type: float Measurement Scale: Units: centimeter Number Type: real Missing Value Code: NA (missing) Attribute Name: W_D3 Attribute Label: Attribute Definition: Bottom soil volume dimension (cm); Depth3: 15-25cm Storage Type: float Measurement Scale: Units: centimeter Number Type: real Missing Value Code: NA (missing) Attribute Name: D_D3 Attribute Label: Attribute Definition: Bottom soil volume dimension (cm) Storage Type: float Measurement Scale: Units: centimeter Number Type: real Missing Value Code: NA (missing) Attribute Name: Fine_P_ug_g Attribute Label: Attribute Definition: Total Phosphorus in fine roots Storage Type: float Measurement Scale: Units: microgramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Coarse_P_ug_g Attribute Label: Attribute Definition: Total Phosphorus in coarse roots Storage Type: float Measurement Scale: Units: microgramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Fine_C_mg_g Attribute Label: Attribute Definition: Total Carbon in fine roots Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Coarse_C_mg_g Attribute Label: Attribute Definition: Total Carbon in coarse roots Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Fine_N_mg_g Attribute Label: Attribute Definition: Total Nitrogen in fine roots Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Coarse_N_mg_g Attribute Label: Attribute Definition: Total Nitrogen in coarse roots Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Fine_P_percent Attribute Label: Attribute Definition: Percent P in fine roots Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Coarse_P_percent Attribute Label: Attribute Definition: Percent P in coarse roots Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Fine_C_percent Attribute Label: Attribute Definition: Percent C in fine roots Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Coarse_C_percent Attribute Label: Attribute Definition: Percent C in coarse roots Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Fine_N_percent Attribute Label: Attribute Definition: Percent N in fine roots Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Coarse_N_percent Attribute Label: Attribute Definition: Percent N in coarse roots Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_P_ug_g Attribute Label: Attribute Definition: Total Phosphorus in roots Storage Type: float Measurement Scale: Units: microgramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_C_mg_g Attribute Label: Attribute Definition: Total Carbon in roots Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_N_mg_g Attribute Label: Attribute Definition: Total Nitrogen in roots Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_P Attribute Label: Attribute Definition: Percent P in roots Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_C Attribute Label: Attribute Definition: Percent C in roots Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Attribute Name: Total_N Attribute Label: Attribute Definition: Percent N in roots Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (missing) Data Table Entity Name: Belowground_biomass_manipulation Entity Description: Belowground_biomass_manipulation Object Name: FCE1226_Belowground_biomass_manipulation.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Bin Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-aboveground sawgrass monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FW= freshwater FWP= freshwater and phosphorus SW= saltwater SWP= saltwater and phosphorus Missing Value Code: Attribute Name: 0to7cm Attribute Label: Attribute Definition: Gram of fine roots in 0-7cm soil column taken at end of experiment Storage Type: float Measurement Scale: Units: gram Number Type: real Missing Value Code: NA (missing) Attribute Name: 7to15cm Attribute Label: Attribute Definition: Gram of fine roots in 7-15cm soil column taken at end of experiment Storage Type: float Measurement Scale: Units: gram Number Type: real Missing Value Code: NA (missing) Attribute Name: 15to25cm Attribute Label: Attribute Definition: Gram of fine roots in 15-25cm soil column taken at end of experiment Storage Type: float Measurement Scale: Units: gram Number Type: real Missing Value Code: NA (missing) Data Table Entity Name: Soil bulk density and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass peat Entity Description: Soil bulk density and the elemental (carbon, nitrogen, and phosphorus) composition of sawgrass peat Object Name: FCE1226_Soil_density_element.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Bin_num Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-soil monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FW= freshwater FWP= freshwater and phosphorus SW= saltwater SWP= saltwater and phosphorus Missing Value Code: Attribute Name: Depth Attribute Label: Attribute Definition: Depth (1: 0-7.5cm, 2: 7.5-15cm, 3: 15-25cm) Storage Type: float Measurement Scale: Units: centimeter Number Type: natural Missing Value Code: NA (Not collected) Attribute Name: Volume Attribute Label: Attribute Definition: Volumes Storage Type: float Measurement Scale: Units: centimeterCubed Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Height Attribute Label: Attribute Definition: Height of soil sample Storage Type: float Measurement Scale: Units: centimeter Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Width Attribute Label: Attribute Definition: Width of soil sample Storage Type: float Measurement Scale: Units: centimeter Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Length Attribute Label: Attribute Definition: Length of soil sample Storage Type: float Measurement Scale: Units: centimeter Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Wet_wt Attribute Label: Attribute Definition: Wet weight Storage Type: float Measurement Scale: Units: kilogram Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Dry_wt Attribute Label: Attribute Definition: Dry weight Storage Type: float Measurement Scale: Units: gram Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Total_P_ug_g Attribute Label: Attribute Definition: Percent P in soil Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Total_C_mg_g Attribute Label: Attribute Definition: Percent C in soil Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Total_N_mg_g Attribute Label: Attribute Definition: Percent N in soil Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Total_P Attribute Label: Attribute Definition: Total P in soil dry weight Storage Type: float Measurement Scale: Units: microgramPerGram Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Total_C Attribute Label: Attribute Definition: Total C in soil dry weight Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Total_N Attribute Label: Attribute Definition: Total N in soil dry weight Storage Type: float Measurement Scale: Units: milligramPerGram Number Type: real Missing Value Code: NA (Not collected) Attribute Name: Bulk_density Attribute Label: Attribute Definition: Bulk density taken at end of experiment Storage Type: float Measurement Scale: Units: gramPerCentimeterCubed Number Type: real Missing Value Code: NA (Not collected) Data Table Entity Name: Sawgrass Aboveground Net Primary Production Entity Description: Sawgrass Aboveground Net Primary Production Object Name: FCE1226_ANPP.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: binnum Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-soil monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FW= freshwater FWP= freshwater and phosphorus SW= saltwater SWP= saltwater and phosphorus Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: ANPP Attribute Label: Attribute Definition: Aboveground net primary production Storage Type: float Measurement Scale: Units: gramPerMeterSquaredPerYear Number Type: real Missing Value Code: Data Table Entity Name: Soil methane flux study during saltwater intrusion experiment Entity Description: Soil methane flux study during saltwater intrusion experiment Object Name: FCE1226_SoilCH4_R.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: binnum Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-soil monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FWX= freshwater FWP= freshwater and phosphorus SWX= saltwater SWP= saltwater and phosphorus Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: SoilCH4 Attribute Label: Attribute Definition: Soil methane flux Storage Type: float Measurement Scale: Units: nanomolesPerMeterSquaredPerSecond Number Type: real Missing Value Code: NA (Not collected) Data Table Entity Name: Soil CO2 flux study during saltwater intrusion experiment Entity Description: Soil CO2 flux study during saltwater intrusion experiment Object Name: FCE1226_SoilCO2_R.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: binnum Attribute Label: Attribute Definition: Bin number (a container used for incubating sawgrass-soil monolith) Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: treatment Attribute Label: Attribute Definition: FW: freshwater, FWP: freshwater and phosphorus, SW: saltwater, SWP: saltwater and phosphorus Storage Type: string Measurement Scale: FWX= freshwater FWP= freshwater and phosphorus SWX= saltwater SWP= saltwater and phosphorus Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: SoilCO2 Attribute Label: Attribute Definition: Soil carbon dioxide flux Storage Type: float Measurement Scale: Units: micromolesPerMeterSquaredPerSecond Number Type: real Missing Value Code: NA (Missing) Data Table Entity Name: Extracellular enzyme activity on root litter and bulk soil during saltwater intrusion experiment Entity Description: Extracellular enzyme activity on root litter and bulk soil during saltwater intrusion experiment Object Name: FCE1226_Enzyme.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: days Attribute Label: Attribute Definition: number of days since the start of the experiment when the enzymes were sampled Storage Type: float Measurement Scale: Units: dimensionless Number Type: natural Missing Value Code: Attribute Name: nut_trt Attribute Label: Attribute Definition: treatement conditons (np - no phosphorus, p - with phosphorus) Storage Type: string Measurement Scale: np= no phosphorus p= with phosphorus Missing Value Code: Attribute Name: water_trt Attribute Label: Attribute Definition: treatment conditions (fresh and salt) Storage Type: string Measurement Scale: fresh= freshwater salt= saltwater Missing Value Code: Attribute Name: trt_label Attribute Label: Attribute Definition: treatment label (fresh.P, fhresh, salt.P, salt) Storage Type: string Measurement Scale: fresh= freshwater fresh.P= freshwater with P salt= saltwater salt.P= saltwater with P Missing Value Code: Attribute Name: phos Attribute Label: Attribute Definition: phosphatase activity (umol/g/h) Storage Type: float Measurement Scale: Units: micromolesPerGramPerHour Number Type: real Missing Value Code: NA (Missing) Attribute Name: ars Attribute Label: Attribute Definition: arylsulfatase activity (umol/g/h) Storage Type: float Measurement Scale: Units: micromolesPerGramPerHour Number Type: real Missing Value Code: NA (Missing) Attribute Name: bg Attribute Label: Attribute Definition: beta-1,4-glucosidsase activity (umol/g/h) Storage Type: float Measurement Scale: Units: micromolesPerGramPerHour Number Type: real Missing Value Code: NA (Missing) Attribute Name: cel Attribute Label: Attribute Definition: beta-1,4-cellobiosidase activity (umol/g/h) Storage Type: float Measurement Scale: Units: micromolesPerGramPerHour Number Type: real Missing Value Code: NA (Missing) Data Table Entity Name: Elemental analysis of periphyton cores Entity Description: Elemental analysis of periphyton cores Object Name: FCE1226_PeriphytonCores_Chemistry.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Sampling_Event Attribute Label: Attribute Definition: Sampling period Storage Type: string Measurement Scale: Sampling period Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Periphyton sample collection date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Mesocosm Attribute Label: Attribute Definition: Mesocosm identification number Storage Type: string Measurement Scale: Mesocosm identification number Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: F: freshwater, FP: freshwater and phosphorus, S: saltwater, SP: saltwater and phosphorus Storage Type: string Measurement Scale: F= freshwater FP= freshwater and phosphorus S= saltwater SP= saltwater and phosphorus Missing Value Code: Attribute Name: TP Attribute Label: Attribute Definition: Periphyton total phosphorus concentration in micrograms per gram of periphyton Storage Type: float Measurement Scale: Units: microgramPerGram Number Type: real Missing Value Code: NA (data not collected) Attribute Name: TN Attribute Label: Attribute Definition: Periphyton total nitrogen concentration in micrograms per gram of periphyton Storage Type: float Measurement Scale: Units: microgramPerGram Number Type: real Missing Value Code: NA (data not collected) Attribute Name: TC Attribute Label: Attribute Definition: Periphyton total carbon concentration in micrograms per gram of periphyton Storage Type: float Measurement Scale: Units: microgramPerGram Number Type: real Missing Value Code: NA (data not collected) Attribute Name: Mineral_Content Attribute Label: Attribute Definition: Periphyton percent inorganic weight of total dry weight Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (data not collected) Attribute Name: Organic_Content Attribute Label: Attribute Definition: Periphyton percent organic weight of total dry weight Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: NA (data not collected) Attribute Name: Chla Attribute Label: Attribute Definition: Periphyton chlorophyll-a concentration in micrograms per unit of ash-free dry mass Storage Type: float Measurement Scale: Units: microgramPerGram Number Type: real Missing Value Code: NA (data not collected) Data Table Entity Name: Periphyton Accumulation Rates Entity Description: Periphyton Accumulation Rates Object Name: FCE1226_PeriphytonPlates_AccumulationRates.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Sampling_Event Attribute Label: Attribute Definition: Sampling period Storage Type: string Measurement Scale: Sampling period Missing Value Code: Attribute Name: Deploy_Date Attribute Label: Attribute Definition: Settlement plate deployment date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Retrieve_Date Attribute Label: Attribute Definition: Settlement plate retrieval data Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Mesocosm Attribute Label: Attribute Definition: Mesocosm identification number Storage Type: string Measurement Scale: Mesocosm identification number Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: F: freshwater, FP: freshwater and phosphorus, S: saltwater, SP: saltwater and phosphorus Storage Type: string Measurement Scale: F= freshwater FP= freshwater and phosphorus S= saltwater SP= saltwater and phosphorus Missing Value Code: Attribute Name: Total_Mass_Accumulation_Rate Attribute Label: Attribute Definition: Periphyton total mass accumulation rate on settlement plates (clear acrylic) in milligrams per meter squared per day Storage Type: float Measurement Scale: Units: milligramsPerMeterSquaredPerDay Number Type: real Missing Value Code: NA (not collected) Attribute Name: Organic_Mass_Accumulation_Rate Attribute Label: Attribute Definition: Periphyton organic mass accumulation rate on settlement plates (clear acrylic) in milligrams per meter squared per day Storage Type: float Measurement Scale: Units: milligramsPerMeterSquaredPerDay Number Type: real Missing Value Code: NA (not collected) Attribute Name: Chla_Accumulation_Rate Attribute Label: Attribute Definition: Periphyton chlorophyll-a accumulation rate on settlement plates (clear acrylic) in micrograms per meter squared per day Storage Type: float Measurement Scale: Units: microgramsPerMeterSquaredPerDay Number Type: real Missing Value Code: NA (not collected) Attribute Name: Chla_Accumulation_Rate_AFDM Attribute Label: Attribute Definition: Periphyton chlorophyll-a accumulation rate on settlement plates (clear acrylic) in micrograms per gram of periphyton ash-free dry mass per day Storage Type: float Measurement Scale: Units: microgramsPerMeterSquaredPerDay Number Type: real Missing Value Code: NA (not collected) Data Table Entity Name: Relative Abundance of Diatoms on PeriphytonPlates Entity Description: Relative Abundance of Diatoms on PeriphytonPlates Object Name: FCE1226_PeriphytonPlates_DiatomRelAbund.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Sampling_Event Attribute Label: Attribute Definition: sampling period Storage Type: string Measurement Scale: sampling period Missing Value Code: Attribute Name: Date Attribute Label: Attribute Definition: Sampling date Storage Type: date Measurement Scale: Missing Value Code: Attribute Name: Mesocosm Attribute Label: Attribute Definition: Mesocosm identification number Storage Type: string Measurement Scale: Mesocosm identification number Missing Value Code: Attribute Name: Treatment Attribute Label: Attribute Definition: F: freshwater, FP: freshwater and phosphorus, S: saltwater, SP: saltwater and phosphorus Storage Type: string Measurement Scale: F= freshwater FP= freshwater and phosphorus S= saltwater SP= saltwater and phosphorus Missing Value Code: Attribute Name: Species Attribute Label: Attribute Definition: Diatom species Storage Type: string Measurement Scale: Diatom species Missing Value Code: Attribute Name: Abundance Attribute Label: Attribute Definition: relative abundance Storage Type: float Measurement Scale: Units: dimensionless Number Type: real Missing Value Code: Methods Method Step Description Dataset: PeriphytonCores_Chemistry Ten mL periphyton mat subsamples were collected bimonthly, starting in April 2015, from each mesocosm using a plastic syringe. In the lab, the mat samples were placed in a 500-mL beaker and homogenized with deionized water (DI) water to a periphyton slurry with a minimum volume of 200-mL. The beaker was placed on a stir plate to facilitate continuous mixing while the slurry was subsampled for biomass, chl-a total carbon (TC), total nitrogen (TN), and total phosphorus (TP) analyses. The biomass and chl-a subsamples were analyzed using the same methods described for the settlement plate samples. The TP subsample was dried at 80 degrees C and pulverized with a mortar and pestle. We processed a known amount of each subsample and used colorimetric analysis to estimate TP concentration, expressed as micrograms gram AFDM, following the methods of Solorzano and Sharp (1980). TC and TN subsamples were ground down to a fine powder by mortar and pestle, dried at 60 degrees C, and analyzed for total C and N content using a Flash 1112 elemental analyzer (CE Elantech, Lakewood, New Jersey, USA) following standard procedures. PeriphytonPlates_AccumulationRates Clear, acrylic settlement plates (approximately 104 square centimeters) were deployed in each mesocosm to measure periphyton biomass and chlorophyll-a accumulation rates. The settlement plates were incubated on the soil surface of the mesocosm for two months to allow periphyton mats in the mesocosms to colonize the plates. At the end of the two-month incubation period, settlement plates were retrieved and transported to the laboratory, where they were scrapped clean using a razor blade and deionized water. Settlement plates were sterilized using a bleach solution and redeployed the following month. This was done quarterly from March 2015 to April 2016 for a total of 4 settlement plate samples per mesocosm over the duration of the experiment. The material scraped from the settlement plates was collected in a beaker and homogenized with deionized water (DI) water to a minimum volume of 200-mL. The beaker was placed on a stir plate to facilitate continuous mixing while the slurry was subsampled for biomass, chlorophyll-a, and diatom analysis. The biomass subsample was placed in a drying oven at 80 degrees C for at least 48 h to obtain a dry mass (g) measurement and then in a furnace where it was combusted at 500 degrees C for 1 h to obtain the ash (mineral) mass (g). We calculated ash-free dry mass (AFDM) by the loss-on-ignition method as the difference between the ash mass and the total dry mass. The chl-a subsample was filtered onto Whatman 25-mm diameter glass fiber filter (GFF) paper, frozen, and later subjected to 48 h 90 percent cold acetone extraction at 20 degrees C. Chl-a concentrations were determined using a Gildford FLUORO IV fluorometer (Gilford Instrument, Oberlin, Ohio, USA; excitation 435 nm; emission 667 nm). AFDM and chl-a accumulation rates (mgPerMeterSquaredPerDay) were calculated by standardizing AFDM and chl-a values by the area of the settlement plates and the number of days incubated. PeriphytonPlates_DiatomRelAbund A subsample of the periphyton material scrapped from the settlement plates was used to analyze diatom species composition. Diatom subsamples were cleaned of mineral debris and organic matter using the sulfuric acid oxidation methods prescribed by Hasle and Fryxell (1970). We pipetted a known volume of cleaned diatom sample onto a glass coverslip, permanently mounted it on a microscope slide with Naphrax (PhycoTech Inc., St. Joseph, Michigan, USA) mounting medium, and viewed the diatoms under a compound light microscope (Axioskop 2; Zeiss, Thornwood, New York) equipped with differential interference contrast and a digital camera (Leica DFC425, Wetzlar, Germany). We counted at least 500 diatom valves along random transects at 600× magnification under oil immersion and identified them to the species level. Raw diatom counts were converted to relative abundance by standardizing the number of valves counted for each species by the total number of valves counted. We chose to analyze periphyton diatom species composition from the settlement plates rather than samples taken from the mesocosm soil surface because, as diatoms are counted and identified from cleaned (oxidized) samples, testing treatment effects on the diatom assemblage using established mats would have been confounded by the presence of dead diatom frustules; using settlement plates allowed us to better pick up diatom community response to treatments over time. Dataset Contact