Relative Abundance of Soft Algae From theComprehensive Everglades Restoration Plan (CERP) Study (FCE) from February 2005 to November 2014
Authors: Evelyn Gaiser
Time period: 2005-02-15 to 2014-11-14
Package id: knb-lter-fce.1212.1
Dataset id: FCE1212
How to cite:
Gaiser, E.. 2017. Relative Abundance of Soft Algae From theComprehensive Everglades Restoration Plan (CERP) Study (FCE) from February 2005 to November 2014. Environmental Data Initiative. https://doi.org/10.6073/pasta/6e16b97781030e670fd94221ac812f5d. Dataset accessed 2021-01-16.
Dataset AbstractRelative soft algae data collected between February 2005 and November 2014
"The Comprehensive Everglades Restoration Plan (CERP) focuses on “getting the water right” in the south Florida ecosystem—getting the right amount of water of the right quality to the right places at the right time" (USACE & DoI, 2015. Central and Southern Florida Project Comprehensive Everglades Restoration Plan) in the Everglades ecosystems. To inform CERP, since February 2005 we have been investigating the spatio-temporal variations of distribution, biomass and diversity of algae (key aquatic primary producers) in periphyton mats in relation to hydrology, nutrients and pH, and other environmental conditions.
Geographic CoverageStudy Extent Description
Between February 2005 and November 2014, samples were taken in the Everglades ecosystem, from LOX to ENP (NE to SW; see Dataset geographic description).
Data were collected in Principal Sampling Units (800m x 800m) in the following regions (see list of sites at row 121). LKO: Lake Okeechobee; LOX: the Arthur R. Marshall Loxahatchee National Wildlife Refuge (Water Conservation Area 1); Pal Mar (PAL); Pennsuco (PEN), Lostman’s Creek (LMC), the ‘Oligohaline’ area (OLG), Southern Marl Prairie (SMP), Shark River Slough (SRS) and Taylor Slough (TSL); Holey Land Wildlife Management Area (HOL), Water Conservation Areas (WCA) 2 and 3.
N: 27.096, S: 25.216, E: -80.150, W: -81.099
Temporal CoverageStart Date: 2005-02-15
End Date: 2014-11-14
Using generalized random-tessellation stratification, we chose a representative set of locations (800 m x 800 m principal sampling units, PSU), within which we sampled three sites in a habitat drawn from a pool of GPS coordinates. At sites where depth was < 1 m, and vegetation not too dense to hamper the formation of periphyton, and our movement in the field, we threw a 1 m3 enclosure, open both at the top and bottom, and collected samples of periphytic algae with a 120 mL plastic beaker from floating or benthic periphyton mats, depending on the depth. Where no mats were present, we took flocculent detritus from the benthos, as this also hosts algae; we then transported the samples back to the laboratory and froze them until analysis. We measured water depth (cm) with a metal ruler, and TP concentrations in the periphyton (μg g−1 dry weight) by means of colorimetry after dry combustion because water column concentrations are often below detection in this extremely oligotrophic wetland [Marazzi et al. (accepted) Freshwater Biology].
We prepared wet mounts from frozen samples and homogenized samples that we then dried onto a cover glass. When CaCO3 crystals were noticeable on the slide, we added a solution of 0.01 mL 10% HCl, dried the cover slip, and inverted it onto a microscope slide in 0.02 ml of water, then sealed it by ringing the glass with fingernail polish. Franco Tobias counted at least 500 algal units (i.e. cells, coenobia, colonies, and filaments of 100µm) at 1000x magnification, using a compound light microscope, and identified them to the lowest possible taxonomic unit (genus, species, or variety). [Marazzi et al. (2017) "Algal richness and life-history strategies are influenced by hydrology and phosphorus in two major subtropical wetlands" Freshwater Biology]
Marazzi, Luca , Evelyn E. Gaiser, Vivienne J. Jones, Franco A. Tobias, A. W. Mackay. 2017. Algal richness and life-history strategies are influenced by hydrology and phosphorus in two major subtropical wetlands. Freshwater Biology, 62(2): 274-290.
Distribution and Intellectual RightsOnline distribution
Data Submission Date: 2016-11-10
These data are classified as 'Type II' whereby original FCE LTER experimental data collected by individual FCE researchers to be released to restricted audiences according to terms specified by the owners of the data. Type II data are considered to be exceptional and should be rare in occurrence. The justification for exceptions must be well documented and approved by the lead PI and Site Data Manager. Some examples of Type II data restrictions may include: locations of rare or endangered species, data that are covered under prior licensing or copyright (e.g., SPOT satellite data), or covered by the Human Subjects Act, Student Dissertation data and those data related to the FCE LTER Program but not funded by the National Science Foundation (NSF) under LTER grants #DEB-9910514, and # DBI-0620409. Researchers that make use of Type II Data may be subject to additional restrictions to protect any applicable commercial or confidentiality interests. All publications based on this dataset must cite the data Contributor, the Florida Coastal Everglades Long-Term Ecological Research (LTER) Program and that this material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DEB-1237517, #DBI-0620409, and #DEB-9910514. Additionally, two copies of the manuscript must be submitted to the Florida Coastal Everglades LTER Program Office, LTER Program Manager, Florida International University, Southeast Environmental Research Center, OE 148, University Park, Miami, Florida 33199. For a complete description of the FCE LTER Data Access Policy and Data User Agreement, please go to FCE Data Management Policy at http://fcelter.fiu.edu/data/DataMgmt.pdf and LTER Network Data Access Policy at http://fcelter.fiu.edu/data/core/data_user_agreement/distribution_policy.html.
KeywordsComprehensive Everglades Restoration Plan (CERP), CERP, ecological research, long-term monitoring, abundance, community composition, algae
Data Table and FormatData Table: Abundance of soft algae from CERP study locations