Metadata

Sampling Description
Using generalized random-tessellation stratification, we chose a representative set of locations (800 m x 800 m principal sampling units, PSU), within which we sampled three sites in a habitat drawn from a pool of GPS coordinates. At sites where depth was < 1 m, and vegetation not too dense to hamper the formation of periphyton, and our movement in the field, we threw a 1 m3 enclosure, open both at the top and bottom, and collected samples of periphytic algae with a 120 mL plastic beaker from floating or benthic periphyton mats, depending on the depth. Where no mats were present, we took flocculent detritus from the benthos, as this also hosts algae; we then transported the samples back to the laboratory and froze them until analysis. We measured water depth (cm) with a metal ruler, and TP concentrations in the periphyton (μg g−1 dry weight) by means of colorimetry after dry combustion because water column concentrations are often below detection in this extremely oligotrophic wetland [Marazzi, L., E.E. Gaiser, V.J. Jones, F. Tobias, A.W. Mackay. 2017. Algal richness and life-history strategies are influenced by hydrology and phosphorus in two major subtropical wetlands. Freshwater Biology 62: 274-290. DOI: 10.1111/fwb.12866 ].

Method Step

Quality Control
Data were entered into excel files and then cross-checked and validated by two individuals before migrating into a database.