Dataset title: Stable isotope values of consumers, producers, and organic matter in the Shark River Slough and Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, 2019 – ongoing Dataset ID: doi:10.6073/pasta/da36c97eccd5461ba32eecce23a53892 Dataset Creator Name: Dr. Ryan Rezek Position: Assistant Professor Organization: Coastal Carolina University Address: 301 Allied Dr. Conway, SC 29576 United States Email: rrezek@coastal.edu Metadata Provider Organization: Florida Coastal Everglades LTER Address: Florida International University 11200 SW 8th Street, OE 148 Miami, FL 33199 USA Phone: 305-348-6054 Email: fcelter@fiu.edu URL: https://fcelter.fiu.edu Dataset Abstract Wetland food webs have often been characterized as detrital-based ‘brown’ energy pyramids, whereas the relative role of autotrophic (‘green’) vs. microbial (‘brown’) energy sources falls along a continuum set by physical drivers, as well as autochthonous and allochthonous inputs (Moore et al. 2004; Evans-White & Halvorson 2017) that change with ecosystem development (Schmitz et al. 2006). In the Florida Coastal Everglades (FCE), metabolic imbalances, including the collapse of calcareous periphyton mats, begin with a loss of foundation species primary production and legacy organic matter (Gaiser et al. 2006). This process likely enhances heterotrophic microbial productivity (Schulte 2016) and the supply of detrital energy to consumers by changing bioavailable and recalcitrant carbon supplies (Baggett et al. 2013). A shift from complex periphyton communities to transient planktonic communities under elevated P exposure reduces habitat structure and animal refuges but increases ‘green’ energy supplies and edibility (Trexler et al. 2015; Naja et al. 2017). Multiple sites (n=9) within the FCE were selected to document changes in coastal food webs as a result of eutrophication and increasing hydrologic variability. The project began in 2019 and is currently ongoing. References: Baggett, L. P., Heck, K. L., Frankovich, T. A., Armitage, A. R., & Fourqurean, J. W. (2013). Stoichiometry, growth, and fecundity responses to nutrient enrichment by invertebrate grazers in sub-tropical turtle grass (Thalassia testudinum) meadows. Marine biology, 160, 169-180. Evans-White, M. A., and H. M. Halvorson. 2017. Comparing the Ecological Stoichiometry in Green and Brown Food Webs – A Review and Meta-analysis of Freshwater Food Webs. Frontiers in Microbiology 8:1184. Gaiser, E. E., Childers, D. L., Jones, R. D., Richards, J. H., Scinto, L. J., & Trexler, J. C. (2006). Periphyton responses to eutrophication in the Florida Everglades: cross‐system patterns of structural and compositional change. Limnology and Oceanography, 51(1part2), 617-630. Moore, J. C., E. L. Berlow, D. C. Coleman, P. C. Ruiter, Q. Dong, A. Hastings, N. C. Johnson, K. S. McCann, K. Melville, P. J. Morin, K. Nadelhoffer, A. D. Rosemond, D. M. Post, J. L. Sabo, K. M. Scow, M. J. Vanni, and D. H. Wall. 2004. Detritus, trophic dynamics and biodiversity: Detritus, trophic dynamics and biodiversity. Ecology Letters 7:584–600. Naja, M., Childers, D. L., & Gaiser, E. E. (2017). Water quality implications of hydrologic restoration alternatives in the Florida Everglades, United States. Restoration Ecology, 25, S48-S58. Schmitz, O. J., Kalies, E. L., & Booth, M. G. (2006). Alternative dynamic regimes and trophic control of plant succession. Ecosystems, 9, 659-672. Schulte, Nicholas O., "Controls on Benthic Microbial Community Structure and Assembly in a Karstic Coastal Wetland" (2016). FIU Electronic Theses and Dissertations. 2447. 10.25148/etd.FIDC000233 Trexler, J. C., Gaiser, E. E., Kominoski, J. S., & Sanchez, J. (2015). The role of periphyton mats in consumer community structure and function in calcareous wetlands: lessons from the Everglades. Microbiology of the everglades ecosystem, 155-179. Geographic Coverage Bounding Coordinates Geographic description: SRS3 West bounding coordinate: -80.85327617 East bounding coordinate: -80.85327617 North bounding coordinate: 25.46820617 South bounding coordinate: 25.46820617 Geographic description: SRS4 West bounding coordinate: -80.96431016 East bounding coordinate: -80.96431016 North bounding coordinate: 25.40976421 South bounding coordinate: 25.40976421 Geographic description: SRS6 West bounding coordinate: -81.07794623 East bounding coordinate: -81.07794623 North bounding coordinate: 25.36462994 South bounding coordinate: 25.36462994 Geographic description: TS/Ph3 West bounding coordinate: -80.66271768 East bounding coordinate: -80.66271768 North bounding coordinate: 25.25240534 South bounding coordinate: 25.25240534 Geographic description: TS/Ph7a West bounding coordinate: -80.63910514 East bounding coordinate: -80.63910514 North bounding coordinate: 25.19080491 South bounding coordinate: 25.19080491 Geographic description: TS/Ph9 West bounding coordinate: -80.48978207 East bounding coordinate: -80.48978207 North bounding coordinate: 25.17692874 South bounding coordinate: 25.17692874 Geographic description: TS/Ph10 West bounding coordinate: -80.68097374 East bounding coordinate: -80.68097374 North bounding coordinate: 25.02476744 South bounding coordinate: 25.02476744 Geographic description: TS/Ph11 West bounding coordinate: -80.93798347 East bounding coordinate: -80.93798347 North bounding coordinate: 24.91293492 South bounding coordinate: 24.91293492 Geographic description: RB10 West bounding coordinate: -80.869183 East bounding coordinate: -80.869183 North bounding coordinate: 25.460931 South bounding coordinate: 25.460931 Geographic description: TS_River West bounding coordinate: -80.849658 East bounding coordinate: -80.849658 North bounding coordinate: 25.216174 South bounding coordinate: 25.216174 Geographic description: TS_Marsh West bounding coordinate: -80.614686 East bounding coordinate: -80.614686 North bounding coordinate: 25.393042 South bounding coordinate: 25.393042 Temporal Coverage Start Date: 2019 End Date: 2022 Data Table Entity Name: FCE_1242 Entity Description: Stable isotope values and nutrient content of consumers, producers, and organic matter in the FCE LTER Object Name: FCE_1242.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: common_name Attribute Label: common name of the species Attribute Definition: common_name Storage Type: string Measurement Scale: text Missing Value Code: Attribute Name: species_name Attribute Label: species scientific name Attribute Definition: species_name Storage Type: string Measurement Scale: text Missing Value Code: NA (not applicable) Attribute Name: season Attribute Label: season in which sampling took place Attribute Definition: sampling_season Storage Type: string Measurement Scale: text Missing Value Code: Attribute Name: year Attribute Label: year Attribute Definition: year Storage Type: dateTime Measurement Scale: Missing Value Code: Attribute Name: site Attribute Label: sampling site Attribute Definition: site Storage Type: string Measurement Scale: text Missing Value Code: Attribute Name: d13C Attribute Label: d13C VPDB x 1000 Attribute Definition: Delta 13 Carbon Storage Type: float Measurement Scale: Units: partsPerThousand Number Type: real Missing Value Code: -9999 (Sample pending analysis) Attribute Name: d15N Attribute Label: d15N AIR x 1000 Attribute Definition: Delta 15 Nitrogen Storage Type: float Measurement Scale: Units: partsPerThousand Number Type: real Missing Value Code: -9999 (Sample pending analysis) Attribute Name: d34S Attribute Label: d34S Attribute Definition: Delta 34 Sulfur Storage Type: float Measurement Scale: Units: partsPerThousand Number Type: real Missing Value Code: -9999 (Sample pending analysis) Attribute Name: N_percent Attribute Label: Attribute Definition: percent nitrogen Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: -9999 (Sample pending analysis) Attribute Name: C_percent Attribute Label: Attribute Definition: percent carbon Storage Type: float Measurement Scale: Units: percent Number Type: real Missing Value Code: -9999 (Sample pending analysis) Attribute Name: S_percent Attribute Label: Attribute Definition: percent sulfur Storage Type: float Measurement Scale: Units: partsPerThousand Number Type: real Missing Value Code: -9999 (Sample pending analysis) Methods Method Step Description Method Step Description Method Step Description Method Step Description Method Step Description Method Step Description Method Step Description Method Step Description Stable Isotope Analysis: Dried samples of producer, consumer, and organic matter were weighed, wrapped, and shipped for analysis: Delta Carbon 13, Delta Nitrogen 15, Delta Sulfur 34, Percent Carbon, Percent Nitrogen, and Percent Sulfur were estimated via mass spectrometry (DELTA Q™ Isotope Ratio Mass Spectrometer, Thermo-Scientific™). Method Step Description Distribution Online distribution: https://pasta.lternet.edu/package/data/eml/knb-lter-fce/1242/4/4530be5d63d34244518b2cf8ae2867c1 Intellectual Rights This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you. Dataset Keywords Nitrogen 15 Carbon 13 Sulfur 34 FCE FCE LTER long term monitoring Florida Coastal Everglades LTER Ecological Research producers stable isotopes consumers populations Maintenance 1242.4: Data updated to include 2022 values and pending samples, standardized file name, improved taxonomic metadata, and revised species name errors. Dataset Contact Name: Dr. Ryan Rezek Position: Assistant Professor Organization: Coastal Carolina University Address: 301 Allied Dr. Conway, SC 29576 United States Email: rrezek@coastal.edu Position: Information Manager Organization: Florida Coastal Everglades LTER Address: Florida International University 11200 SW 8th Street, OE 148 Miami, FL 33199 USA Email: fcelter@fiu.edu URL: https://fcelter.fiu.edu Name: James Sturges Organization: Florida International University Email: jstur015@fiu.edu