Dataset title: Periphyton and Associated Environmental Data From the Comprehensive Everglades Restoration Plan (CERP) Study from February 2005 to November 2014 (FCE) Dataset ID: FCE1210_CERP_PeriphytonEnv Research type: Long-term Dataset Creator Name: Dr. Evelyn Gaiser Position: Primary Investigator Organization: Florida Coastal Everglades LTER Program Address: 11200 S.W. 8th Street Miami, Florida 33199 USA Phone: 305-348-6154 Fax: 305-348-4096 Email: gaiser@fiu.edu URL: http://algae.fiu.edu Metadata Provider Organization: Florida Coastal Everglades LTER Program Address: Florida International University University Park OE 148 Miami, FL 33199 USA Phone: 305-348-6054 Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Dataset Abstract Periphyton data and environmental data collected between February 2005 to November 2014 "The Comprehensive Everglades Restoration Plan (CERP) focuses on “getting the water right” in the south Florida ecosystem—getting the right amount of water of the right quality to the right places at the right time" (USACE & DoI, 2015. Central and Southern Florida Project Comprehensive Everglades Restoration Plan) in the Everglades ecosystems. To inform CERP, since February 2005 we have been investigating the spatio-temporal variations of distribution, biomass and diversity of algae (key aquatic primary producers) in periphyton mats in relation to hydrology, nutrients and pH, and other environmental conditions. Geographic Coverage Study Extent Description Between February 2005 and November 2014, samples were taken in the Everglades ecosystem, from LOX to ENP (NE to SW; see Dataset geographic description). Bounding Coordinates Geographic description: Data were collected in Principal Sampling Units (800m x 800m) in the following regions (see list of sites at row 121). LKO: Lake Okeechobee; LOX: the Arthur R. Marshall Loxahatchee National Wildlife Refuge (Water Conservation Area 1); Pal Mar (PAL); Pennsuco (PEN), Lostman’s Creek (LMC), the ‘Oligohaline’ area (OLG), Southern Marl Prairie (SMP), Shark River Slough (SRS) and Taylor Slough (TSL); Holey Land Wildlife Management Area (HOL), Water Conservation Areas (WCA) 2 and 3. West bounding coordinate: -81.099 East bounding coordinate: -80.150 North bounding coordinate: 27.096 South bounding coordinate: 25.216 All Sites Geographic Description:CERP Study Area Longitude:25.216 Latitude:27.096 Temporal Coverage Start Date: 2005-02-15 End Date: 2014-11-14 Data Table Entity Name: FCE1210_CERP_PeriphytonEnv Entity Description: Periphyton data collected for CERP study by FCE LTER personnel Object Name: FCE1210_CERP_Periphyton.txt Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Date Attribute Label: sampling time details Attribute Definition: Sampling date Storage Type: DateTime Measurement Scale: Missing Value Code: Attribute Name: Year Attribute Label: sampling time details Attribute Definition: Year sites were sampled Storage Type: DateTime Measurement Scale: Missing Value Code: Attribute Name: Season Attribute Label: sampling time details Attribute Definition: Wet or dry season Storage Type: Nominal Measurement Scale: Wet or dry season Missing Value Code: Attribute Name: PSU Attribute Label: location Attribute Definition: Principal Sampling Unit Storage Type: Nominal Measurement Scale: Principal Sampling Unit Missing Value Code: Attribute Name: Region Attribute Label: Sampling region Attribute Definition: Region designation Storage Type: Nominal Measurement Scale: Region designation Missing Value Code: Attribute Name: Draw Attribute Label: A1-A2 Attribute Definition: draw sampled Storage Type: Nominal Measurement Scale: draw sampled Missing Value Code: Attribute Name: Replicate Attribute Label: replicate Attribute Definition: Throw 1, 2 or 3 Storage Type: Nominal Measurement Scale: Throw 1, 2 or 3 Missing Value Code: Attribute Name: Sample_code Attribute Label: Sample code Attribute Definition: Sample code Storage Type: Nominal Measurement Scale: Sample code Missing Value Code: Attribute Name: Depth_cm Attribute Label: water Attribute Definition: Average water depth Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Temperature_C Attribute Label: water Attribute Definition: Temperature of water sample Storage Type: Data Measurement Scale: Units celsius Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: pH Attribute Label: water Attribute Definition: pH of water sample Storage Type: Data Measurement Scale: Units dimensionless Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Conductivity_µs/cm Attribute Label: water Attribute Definition: Conductivity of water sample Storage Type: Data Measurement Scale: Units: dimensionless Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Soil_depth_cm Attribute Label: soil Attribute Definition: Average depth of soil at throw Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken or greater than 100) Attribute Name: Soil_type Attribute Label: soil Attribute Definition: Type of soil (rock, marl or peat) Storage Type: Nominal Measurement Scale: Type of soil (rock, marl or peat) Missing Value Code: Attribute Name: Mat_type Attribute Label: periphyton Attribute Definition: Type of mat (calcareous, mix or organic) Storage Type: Nominal Measurement Scale: Type of mat (calcareous, mix or organic) Missing Value Code: Attribute Name: Periphyton_+_Subm_mL Attribute Label: periphyton Attribute Definition: Total biovolume of periphyton + submersed vegetation Storage Type: Data Measurement Scale: Units: milliliter Number Type: real Missing Value Code: Attribute Name: Periphyton_mL Attribute Label: periphyton Attribute Definition: Total biovolume of periphyton only Storage Type: Data Measurement Scale: Units: milliliter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Periphyton_subsample_mL Attribute Label: periphyton Attribute Definition: Volume of periphyton brought back for analysis Storage Type: Data Measurement Scale: Units: milliliter Number Type: real Missing Value Code: 7777 (not available) Attribute Name: Total_Periphyton_Cover Attribute Label: periphyton Attribute Definition: Total periphyton aerial cover Storage Type: Data Measurement Scale: Units: percent Number Type: real Missing Value Code: 777 (less than one) Attribute Name: Quantitative Attribute Label: periphyton Attribute Definition: Determination of measurable periphyton Storage Type: Data Measurement Scale: Yor N Missing Value Code: 9999 (not taken) Attribute Name: Benthic_% Attribute Label: periphyton Attribute Definition: % of sample comprised of benthic periphyton Storage Type: Data Measurement Scale: Units: percent Number Type: natural Missing Value Code: Attribute Name: Bottom_periphyton_% Attribute Label: periphyton Attribute Definition: % of sediment covered by periphyton Storage Type: Data Measurement Scale: Units: percent Number Type: natural Missing Value Code: Attribute Name: %_Plant_attached_algae Attribute Label: periphyton Attribute Definition: % of sample comprised of epiphytic periphyton Storage Type: Data Measurement Scale: Units: percent Number Type: natural Missing Value Code: 9999 (not taken) Attribute Name: %_Plants_w_attached_algae Attribute Label: periphyton Attribute Definition: % of macrophytes covered by epiphytic periphyton Storage Type: Data Measurement Scale: Units: percent Number Type: natural Missing Value Code: 9999 (not taken) Attribute Name: Plant_Sp1 Attribute Label: plant species Attribute Definition: Macrophyte species 1 Storage Type: Nominal Measurement Scale: Macrophyte species 1 Missing Value Code: 9999 (not taken) Attribute Name: Sweater_Sp2 Attribute Label: plant species Attribute Definition: Macrophyte species 2 Storage Type: Nominal Measurement Scale: Macrophyte species 2 Missing Value Code: Attribute Name: Plant_Sp3 Attribute Label: plant species Attribute Definition: Macrophyte species 3 Storage Type: Nominal Measurement Scale: Macrophyte species 3 Missing Value Code: Attribute Name: Floating_% Attribute Label: periphyton Attribute Definition: % of sample comprised of floating periphyton Storage Type: Data Measurement Scale: Units: percent Number Type: natural Missing Value Code: Attribute Name: Water_floating_peri_% Attribute Label: periphyton Attribute Definition: % of water surface covered by floating periphyton Storage Type: Data Measurement Scale: Units: percent Number Type: natural Missing Value Code: 555 (less than five) Attribute Name: Floating_Sp1 Attribute Label: plant species Attribute Definition: Submersed macrophyte species 1 Storage Type: Nominal Measurement Scale: Submersed macrophyte species 1 Missing Value Code: Attribute Name: Floating_Sp2 Attribute Label: plant species Attribute Definition: Submersed macrophyte species 2 Storage Type: Nominal Measurement Scale: Submersed macrophyte species 2 Missing Value Code: Attribute Name: Green_algae_% Attribute Label: plant species Attribute Definition: % of sample comprised of green algae Storage Type: Data Measurement Scale: Units: percent Number Type: natural Missing Value Code: Attribute Name: Green_sp1 Attribute Label: plant species Attribute Definition: Submersed macrophyte species 1 Storage Type: nominal Measurement Scale: Submersed macrophyte species 1 Missing Value Code: Attribute Name: Floc_% Attribute Label: floc Attribute Definition: % of sample comprised of floc Storage Type: Data Measurement Scale: Units: percent Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: P_% Attribute Label: phosphorus Attribute Definition: % phosphorus Storage Type: Data Measurement Scale: Units: percent Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: TP_ug/g Attribute Label: phosphorus Attribute Definition: Total phosporus per gram of dry material Storage Type: Data Measurement Scale: Units: partsPerMillion Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Periphyton_rel_to_plant_% Attribute Label: periphyton Attribute Definition: Proportion of periphyton in sample Storage Type: Data Measurement Scale: Units: percent Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Periphyton_DW_g/m2 Attribute Label: biomass Attribute Definition: Dry weight of periphyton per square meter Storage Type: Data Measurement Scale: Units: gramsPerSquareMeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Periphyton_AFDW_g/m2 Attribute Label: biomass Attribute Definition: Ash-free dry weight of periphyton per square meter Storage Type: Data Measurement Scale: Units: gramsPerSquareMeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Organic_content_% Attribute Label: organic content Attribute Definition: % organic content of sample Storage Type: Data Measurement Scale: Units: percent Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Chl_a_ug/m2 Attribute Label: biomass Attribute Definition: Amount of chlorophyll a per square meter Storage Type: Data Measurement Scale: Units: microgramsPerSquareMeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Easting Attribute Label: Coordinate Attribute Definition: Coordinate Storage Type: Coordinate Measurement Scale: Units: meter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Northing Attribute Label: Coordinate Attribute Definition: Coordinate Storage Type: Coordinate Measurement Scale: Units: meter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Time Attribute Label: sampling time details Attribute Definition: sampling time details Storage Type: DateTime Measurement Scale: Missing Value Code: 9999 (not taken) Attribute Name: Plant_cover_% Attribute Label: plant information Attribute Definition: plant information Storage Type: Data Measurement Scale: Units: percent Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Plant_height Attribute Label: plant information Attribute Definition: plant information Storage Type: Data Measurement Scale: Units: percent Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Depth_ave_30day Attribute Label: hydrology Attribute Definition: average depth for past 30 days Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Depth_ave_60day Attribute Label: hydrology Attribute Definition: average depth for past 60 days Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Depth_ave_90day Attribute Label: hydrology Attribute Definition: average depth for past 90 days Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Depth_ave_180day Attribute Label: hydrology Attribute Definition: average depth for past 180 days Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Depth_ave_365day Attribute Label: hydrology Attribute Definition: average depth for past 365 days Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Depth_offset Attribute Label: hydrology Attribute Definition: corrected - Eden depth Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Eden_depth Attribute Label: hydrology Attribute Definition: eden data Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Corrected_depth Attribute Label: hydrology Attribute Definition: eden depth corrected to depth taken at sampling event Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Dsldd Attribute Label: hydrology Attribute Definition: number of days since depth<=5cm Storage Type: Data Measurement Scale: Units: count Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Drylen Attribute Label: hydrology Attribute Definition: dry period length Storage Type: Data Measurement Scale: Units: count Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Lastdaydry Attribute Label: hydrology Attribute Definition: day Storage Type: Data Measurement Scale: Units: count Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Yearlength Attribute Label: hydrology Attribute Definition: number of days Storage Type: Data Measurement Scale: Units: count Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Hydroperiod Attribute Label: hydrology Attribute Definition: number of days depth >5 since Januray 1 Storage Type: Data Measurement Scale: Units: count Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Depthlag30 Attribute Label: hydrology Attribute Definition: depth from 30 days ago Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Depthlag60 Attribute Label: hydrology Attribute Definition: depth from 60 days ago Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Depthlag90 Attribute Label: hydrology Attribute Definition: depth from 90 days ago Storage Type: Data Measurement Scale: Units: centimeter Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Hydroperiod_annual Attribute Label: hydrology Attribute Definition: number of days depth>5cm for the calendar year of sample Storage Type: Data Measurement Scale: Units: count Number Type: real Missing Value Code: 9999 (not taken) Attribute Name: Hydroperiod_mean Attribute Label: hydrology Attribute Definition: average of Hydroperiod_Annual at the PSU over the timeseries 2005-20013 Storage Type: Data Measurement Scale: Units: count Number Type: real Missing Value Code: 9999 (not taken) Methods Sampling Description Using generalized random-tessellation stratification, we chose a representative set of locations (800 m x 800 m principal sampling units, PSU), within which we sampled three sites in a habitat drawn from a pool of GPS coordinates. At sites where depth was < 1 m, and vegetation not too dense to hamper the formation of periphyton, and our movement in the field, we threw a 1 m3 enclosure, open both at the top and bottom, and collected samples of periphytic algae with a 120 mL plastic beaker from floating or benthic periphyton mats, depending on the depth. Where no mats were present, we took flocculent detritus from the benthos, as this also hosts algae; we then transported the samples back to the laboratory and froze them until analysis. We measured water depth (cm) with a metal ruler, and TP concentrations in the periphyton (μg g−1 dry weight) by means of colorimetry after dry combustion because water column concentrations are often below detection in this extremely oligotrophic wetland [Marazzi et al. (accepted) Freshwater Biology]. Method Step Description A 120 mL sample of periphyton was collected at each site. Percent aerial cover of periphyton was estimated visually in the field. Ash-free dry mass per area (g/m2), Chlorophyll a (ug/m2), TP (ug/g), % organic content were measured in the laboratory, and water temperature, water depth, conductivity, and pH in the field. Hydroperiod data come from the Everglades Depth Estimation Network (EDEN; http://sofia.usgs.gov/eden/). Information on mat type, soil type, and plants to which periphytic algae were attached was also gathered from field observations. Quality Control Data were thoroughly checked by Luca Marazzi and Franco Tobias. Distribution Online distribution: http://fcelter.fiu.edu/perl/public_data_download.pl?datasetid=FCE1210_CERP_PeriphytonEnv.txt Intellectual Rights These data are classified as 'Type II' whereby original FCE LTER experimental data collected by individual FCE researchers to be released to restricted audiences according to terms specified by the owners of the data. Type II data are considered to be exceptional and should be rare in occurrence. The justification for exceptions must be well documented and approved by the lead PI and Site Data Manager. Some examples of Type II data restrictions may include: locations of rare or endangered species, data that are covered under prior licensing or copyright (e.g., SPOT satellite data), or covered by the Human Subjects Act, Student Dissertation data and those data related to the FCE LTER Program but not funded by the National Science Foundation (NSF) under LTER grants #DEB-9910514, and # DBI-0620409. Researchers that make use of Type II Data may be subject to additional restrictions to protect any applicable commercial or confidentiality interests. All publications based on this dataset must cite the data Contributor, the Florida Coastal Everglades Long-Term Ecological Research (LTER) Program and that this material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DEB-1237517, #DBI-0620409, and #DEB-9910514. Additionally, two copies of the manuscript must be submitted to the Florida Coastal Everglades LTER Program Office, LTER Program Manager, Florida International University, Southeast Environmental Research Center, OE 148, University Park, Miami, Florida 33199. For a complete description of the FCE LTER Data Access Policy and Data User Agreement, please go to FCE Data Management Policy at http://fcelter.fiu.edu/data/DataMgmt.pdf and LTER Network Data Access Policy at http://fcelter.fiu.edu/data/core/data_user_agreement/distribution_policy.html. Dataset Keywords Comprehensive Everglades Restoration Plan (CERP) CERP ecological research long-term monitoring FCE restoration primary production algae aquatic ecosystems Data Submission Date: 2016-11-10 Maintenance First archive of this data set. Dataset Contact Position: Information Manager Organization: Florida Coastal Everglades LTER Program Address: Florida International University University Park OE 148 Miami, FL 33199 USA Phone: 305-348-6054 Fax: 305-348-4096 Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Dataset Submission Date 2016-11-10