Dataset title: Cichlasoma urophthalmus microsatellite fragment size collected from the Florida Everglades (FCE) and Central America from June 2010 to March 2013 Dataset ID: ST_TDCS_Harrison_001 Research type: Short-term Dataset Creator Name: Dr. Elizabeth Harrison Position: Assistant Professor Organization: Florida Coastal Everglades LTER Program Address: Georgia Gwinnet College 1000 University Center Lane I-3108 Lawrenceville, GA 30043 USA Phone: 404-450-8111 Email: eharrison1@ggc.edu Name: Dr. Joel Trexler Position: Director of Marine Science Organization: Florida Coastal Everglades LTER Program Address: 3000 NE 151 St North Miami, FL 33181 USA Phone: 305-348-1966 Fax: 305-919-4030 Email: trexlerj@fiu.edu URL: http://faculty.fiu.edu/~trexlerj/research_interests.htm Metadata Provider Organization: Florida Coastal Everglades LTER Program Address: Florida International University University Park OE 148 Miami, FL 33199 USA Phone: 305-348-6054 Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Dataset Abstract Fragment sizes for 17 microsatellite markers obtained from samples of Cichlasoma urophthalmus collected from fin clippings of fish caught by angling and/or cast netting within the Florida Everglades and Central America. DNA was extracted, amplified and sizes of microsatellite fragments were rounded to nearest unit. Data were used to determine population genetic structure using GenAlex, Structure and DIYABC programs. Geographic Coverage Study Extent Description ENP, west coast of south Florida, Mexico, Belize, Honduras, Guatemala, and Nicaragua Bounding Coordinates Geographic description: Data were collected within ENP, in urban Miami canals and in Mexico, Belize, Guatemala, Honduras, and Nicaragua West bounding coordinate: -81.850 East bounding coordinate: -81.771 North bounding coordinate: 26.634 South bounding coordinate: 26.203 Geographic description: Florida Coastal Everglades LTER Study Area: South Florida, Everglades National Park, and Florida Bay West bounding coordinate: -81.078 East bounding coordinate: -80.490 North bounding coordinate: 25.761 South bounding coordinate: 24.913 Geographic description: Mexico West bounding coordinate: -91.583 East bounding coordinate: -88.197 North bounding coordinate: 18.709 South bounding coordinate: 24.692 Geographic description: Belize West bounding coordinate: -88.494 East bounding coordinate: -88.192 North bounding coordinate: 17.625 South bounding coordinate: 17.021 Geographic description: Guatemala West bounding coordinate: -89.891 East bounding coordinate: -89.640 North bounding coordinate: 16.929 South bounding coordinate: 16.973 Geographic description: Honduras West bounding coordinate: -88.216 East bounding coordinate: -85.912 North bounding coordinate: 15.848 South bounding coordinate: 15.092 Geographic description: Nicaragua West bounding coordinate: -83.571 East bounding coordinate: -83.305 North bounding coordinate: 13.414 South bounding coordinate: 14.187 All Sites Geographic Description:LoopRd Longitude:-80.753 Latitude:25.782 Geographic Description:L31W Longitude:-80.552 Latitude:25.470 Geographic Description:TaftPalm Longitude:-80.268 Latitude:26.043 Geographic Description:216canal Longitude:-80.033 Latitude:25.787 Geographic Description:Aerojet Longitude:-80.527 Latitude:25.390 Geographic Description:MiamiSprings Longitude:-80.279 Latitude:25.835 Geographic Description:WhiskeyCr Longitude:-81.850 Latitude:26.634 Geographic Description:NaplesBay Longitude:-81.771 Latitude:26.203 Geographic Description:SpringCr Longitude:-81.835 Latitude:26.423 Geographic Description:ChetumalRiv Longitude:-88.22 Latitude:17.51 Geographic Description:CrookedTree Longitude:-88.502 Latitude:17.852 Geographic Description:Dangriga Longitude:-88.192 Latitude:17.020 Geographic Description:Fenix Longitude:-91.582 Latitude:18.709 Geographic Description:La Bocana Longitude:-89.944 Latitude:23.215 Geographic Description:LagDeGua Longitude:-85.912 Latitude:15.092 Geographic Description:Macan Longitude:-89.640 Latitude:16.972 Geographic Description:NewRiver Longitude:-88.494 Latitude:17.625 Geographic Description:Nica1 Longitude:-83.571 Latitude:13.414 Geographic Description:Nica3 Longitude:-83.305 Latitude:14.187 Geographic Description:Nica4 Longitude:-83.463 Latitude:14.041 Geographic Description:Omoa Longitude:-88.216 Latitude:15.848 Geographic Description:Peten Longitude:-89.891 Latitude:16.929 Geographic Description:RiaCel Longitude:-90.343 Latitude:20.904 Geographic Description:RiaLag Longitude:-88.157 Latitude:21.598 Geographic Description:StJohnsCollege Longitude:-88.196 Latitude:17.517 Geographic Description:VernonSt Longitude:-88.193 Latitude:17.501 Geographic Description:Xtoloc Longitude:-88.569 Latitude:20.680 Geographic Description:YaBalHa Longitude:-88.074 Latitude:21.566 Geographic Description:Zaci Longitude:-88.197 Latitude:24.692 Temporal Coverage Start Date: 2010-06-17 End Date: 2013-03-09 Data Table Entity Name: ST_TDCS_Harrison_001 Entity Description: Cichlasoma urophthalmus microsatellite fragment size collected from the Florida Everglades (FCE) and Central America Object Name: ST_TDCS_Harrison_001 Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: SampleID Attribute Label: Sample ID Attribute Definition: Sample ID Storage Type: text Measurement Scale: Sample ID Missing Value Code: Attribute Name: Date Attribute Label: Date Attribute Definition: Collection Date Storage Type: datetime Measurement Scale: Missing Value Code: Attribute Name: SITENAME Attribute Label: sitename Attribute Definition: Name of Collection site Storage Type: text Measurement Scale: Name of Collection site Missing Value Code: Attribute Name: Latitude_DD Attribute Label: Latitude_DD Attribute Definition: latitude of collection location in decimal degrees Storage Type: ordinal Measurement Scale: latitude of collection location in decimal degrees Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: Longitude_DD Attribute Label: Longitude_DD Attribute Definition: longitude of collection location in decimal degrees Storage Type: ordinal Measurement Scale: longitude of collection location in decimal degrees Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: Ciur2a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur2b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur4a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur4b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur7a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur7b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur9a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur9b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur10a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur10b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur15a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur15b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur17a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur17b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur21a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur21b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur22a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur22b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur23a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur23b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur31a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur31b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur38a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur38b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur39a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur39b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur41a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur41b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur42a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur42b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur43a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur43b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur46a Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Attribute Name: Ciur46b Attribute Label: Microsatellite marker Attribute Definition: Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele. Storage Type: data Measurement Scale: Units number Precision:1 Number Type: whole Missing Value Code: -9999 (Fragment size could not be determined) Methods Sampling Description Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and sizes of microsatellite fragments were rounded to nearest unit. Data were used to determine population genetic structure using GenAlex, Structure and DIYABC programs Method Step Description Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and sizes of microsatellite fragments were rounded to nearest unit. Data were used to determine population genetic structure using GenAlex, Structure and DIYABC programs Citation Harrison, Elizabeth 2014-09-03. Genetic evidence for multiple sources of the non-native fish Cichlasoma urophthalmus (Günther; Mayan Cichlids) in southern Florida. Plos One, 9(9): doi:10.1371/journal.pone.0104173. Instrumentation cast netting GenAlex Structure and DIYABC programs Quality Control Data was checked and double-checked for errors Distribution Online distribution: http://fcelter.fiu.edu/perl/public_data_download.pl?datasetid=ST_TDCS_Harrison_001.txt Intellectual Rights These data are classified as 'Type I' whereby original FCE LTER experimental data collected by individual FCE researchers are available for scientific purposes 2 years after collection. Maximum lock period = 2 years. All publications based on this dataset must cite the data Contributor, the Florida Coastal Everglades Long-Term Ecological Research (LTER) Program and that this material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DBI-0620409, and #DEB-9910514. Additionally, two copies of the manuscript must be submitted to the Florida Coastal Everglades LTER Program Office, LTER Program Manager, Florida International University, Southeast Environmental Research Center, OE 148, University Park, Miami, Florida 33199. For a complete description of the FCE LTER Data Distribution and Data User Agreement, please go to FCE Data Management Policy at http://fcelter.fiu.edu/data/DataMgmt.pdf and LTER Network Data Access Policy at http://fcelter.fiu.edu/data/core/data_user_agreement/distribution_policy.html. Dataset Keywords FCE Florida Coastal Everglades LTER ecological research long-term monitoring Cichlasoma urophthalmus microsatellite fragment size microsatellites Central America DNA South Florida urban Miami Miami canals consumers fish Data Submission Date: 2014-11-04 Maintenance This is a short-term consumer dataset. Dataset Contact Position: Information Manager Organization: LTER Network Office Address: UNM Biology Department, MSC03-2020 1 University of New Mexico Albuquerque, NM 87131-0001 USA Phone: 505 277-2535 Fax: 505 277-2541 Email: tech-support@lternet.edu URL: http://www.lternet.edu Position: Information Manager Organization: Florida Coastal Everglades LTER Program Address: Florida International University University Park OE 148 Miami, FL 33199 USA Phone: 305-348-6054 Fax: 305-348-4096 Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Dataset Submission Date 2014-11-04 Data Submission Notes Paper Published with these data: Harrison, E., J. Lorenz, J.C. Trexler. 2013. Per Capita Effects of Non-native Mayan Cichlids (Cichlasoma urophthalmus; Gunther) on Native Fish in the Estuarine Southern Everglades. Copeia 2013: 80-96. DOI: 10.1643/CE-11-182. These data related to FCE dataset LT_TDCS_Harrison_002.Data file formatted to be easily read by Genetic programs. Collection dates included in this metadata file under Dataset Information Management Notes.