Dataset title: Periphyton Net Primary Productivity and Respiration Rates from the Taylor Slough, just outside Everglades National Park, South Florida (FCE) from December 1998 to August 2002 Dataset ID: ST_PP_Iwaniec_001 Research type: Short-term Dataset Creator Name: Dr. Tiffany Troxler Position: Project Collaborator Organization: Southeast Environmental Research Center Address: Florida International University University Park Miami, FL 33199 USA Phone: 305-348-1453 Email: troxlert@fiu.edu URL: http://wetland.fiu.edu/ Name: Dr. Daniel Childers Position: Associate Director for Research| Professor Organization: Global Institute of Sustainability| School of Sustainability Address: Arizona State University PO Box 875402 Tempe, AZ 85287 USA Phone: 480-965-2320 Email: dan.childers@asu.edu Metadata Provider Organization: Florida Coastal Everglades LTER Address: Florida International University 11200 SW 8th Street, OE 148 Miami, FL 33199 USA Phone: 305-348-6054 Email: fcelter@fiu.edu URL: https://fcelter.fiu.edu Dataset Abstract Once per year, at TS/Ph-4 and TS/Ph-5 we incubate periphyton from each site in water from each site in a complete factorial design. We quantify metabolic rates of periphyton assemblages using standard oxygen change techniques in 300mL light and dark BOD bottles in triplicate. Using YSI dissolved oxygen probes and meters, we measure dissolved oxygen change as the difference of initial and final oxygen concentrations over a two hour incubation. We calculate net primary productivity and respiration rates in units of oxygen and carbon, then normalize to the organic content (ash free dry weight) of the incubated periphyton. Geographic Coverage Study Extent Description The Study Extent of this dataset includes two FCE Taylor Slough research sites just outside Everglades National Park, South Florida Bounding Coordinates Geographic description: TS/Ph4 West bounding coordinate: -80.52209443 East bounding coordinate: -80.52209443 North bounding coordinate: 25.31472178 South bounding coordinate: 25.31472178 Geographic description: TS/Ph5 West bounding coordinate: -80.52023732 East bounding coordinate: -80.52023732 North bounding coordinate: 25.29478536 South bounding coordinate: 25.29478536 FCE LTER Sites: TS/Ph4,TS/Ph5 Temporal Coverage Start Date: 1998-12-08 End Date: 2002-08-29 Data Table Entity Name: ST_PP_Iwaniec_001 Entity Description: Periphyton Net Primary Productivity and Respiration Rates from the Taylor Slough, just outside Everglades National Park, South Florida (FCE) Object Name: ST_PP_Iwaniec_001.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: PeriphytonSource Attribute Label: sitename Attribute Definition: Name of LTER site where periphyton were collected Storage Type: text Measurement Scale: text Missing Value Code: Attribute Name: WaterSource Attribute Label: sitename Attribute Definition: Name of LTER site where water were collected Storage Type: text Measurement Scale: text Missing Value Code: Attribute Name: Date Attribute Label: date Attribute Definition: Collection date Storage Type: datetime Measurement Scale: Missing Value Code: Attribute Name: NPP Attribute Label: net primary productivity Attribute Definition: Net primary productivity Storage Type: data Measurement Scale: Units: milligramsPerGramPerHour Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded) Attribute Name: NPP_STD Attribute Label: standard deviation Attribute Definition: Periphyton net primary productivity standard deviation Storage Type: data Measurement Scale: Units: milligramsPerGramPerHour Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded) Attribute Name: RESP Attribute Label: respiration rate Attribute Definition: Periphyton respiration rate Storage Type: data Measurement Scale: Units: milligramsPerGramPerHour Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded) Attribute Name: RESP_STD Attribute Label: standard deviation Attribute Definition: Periphyton respiration rate standard deviation Storage Type: data Measurement Scale: Units: milligramsPerGramPerHour Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded) Methods Sampling Description Once per year, at TS/Ph-4 and TS/Ph-5 we incubate periphyton from each site in water from each site in a complete factorial design in triplicate. We quantify metabolic rates of periphyton assemblages using thirty-six 300mL light/dark BOD bottles. From each site, approxiamately 500 mm^2 of periphyton is added to water from each site in a matrix design, the remaining 12 bottles are controls. Combinations for the matrix design that are used with both light and dark bottles include: TS/Ph4 Periphyton x TS/Ph4 Water ; TS/Ph4 Periphyton x TS/Ph5 Water; TS/Ph5 Periphyton x TS/Ph4 Water; TS/Ph5 Periphyton x TS/Ph5 Water; then controls soley have ambient water. Using YSI dissolved oxygen probes and meters, initial and final dissolved oxygen concentrations and times for all bottles is measured during incubation (approxiametely two hours). Incubated periphyton is brought to the lab and dried at 70 C for a minimum of 24 hours then ashed at 500 C for 3 hours. We calculate net primary productivity and respiration rates using standard oxygen change techniques to units of carbon, then normalize to the organic content (using the difference of dry weight and ash free dry weight, AFDW) of the incubated periphyton. Method Step Description Once per year, at TS/Ph-4 and TS/Ph-5 we incubate periphyton from each site in water from each site in a complete factorial design in triplicate. We quantify metabolic rates of periphyton assemblages using thirty-six 300mL light/dark BOD bottles. From each site, approxiamately 500 mm^2 of periphyton is added to water from each site in a matrix design, the remaining 12 bottles are controls. Combinations for the matrix design that are used with both light and dark bottles include: TS/Ph4 Periphyton x TS/Ph4 Water ; TS/Ph4 Periphyton x TS/Ph5 Water; TS/Ph5 Periphyton x TS/Ph4 Water; TS/Ph5 Periphyton x TS/Ph5 Water; then controls soley have ambient water. Using YSI dissolved oxygen probes and meters, initial and final dissolved oxygen concentrations and times for all bottles is measured during incubation (approxiametely two hours). Incubated periphyton is brought to the lab and dried at 70 C for a minimum of 24 hours then ashed at 500 C for 3 hours. We calculate net primary productivity and respiration rates using standard oxygen change techniques to units of carbon, then normalize to the organic content (using the difference of dry weight and ash free dry weight, AFDW) of the incubated periphyton. Instrumentation YSI 5905 BOD Probe, YSI 52 Dissolved Oxygen Meter, 300 mL light and dark BOD bottles, PC Distribution Online distribution: https://pasta.lternet.edu/package/data/eml/knb-lter-fce/1158/5/9a323f60afd16d8c59f5af261e4f5e67 Intellectual Rights This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. 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Dataset Keywords primary production FCE Florida Coastal Everglades LTER ecological research long-term monitoring Everglades National Park primary productivity net primary productivity Taylor Slough periphyton water productivity oxygen incubation light respiration respiration rates metabolic rates NPP RESP Data Submission Date: 2005-04-01 Maintenance knb-lter-fce.1158.5: Updated metadata to EML 2.2.0, added creator ORCID and organization ROR ids, updated project information with award elements; added .csv extension to data file name (no changes to data) Dataset Contact Name: Rafael Travieso Position: South Florida Wetland Ecosystems Lab Field Technican Organization: Southeast Environmental Research Center Address: Florida International University University Park Miami, FL 33199 USA Phone: 305-348-7286 Email: travieso@fiu.edu URL: http://www.fiu.edu/~ecosyst/ Position: Information Manager Organization: Florida Coastal Everglades LTER Address: Florida International University 11200 SW 8th Street, OE 148 Miami, FL 33199 USA Email: fcelter@fiu.edu URL: https://fcelter.fiu.edu Dataset Submission Date 2005-04-01 Dataset Anomalies Experiment not conducted in 2001, therefore no data for year. Information Management Notes This is a short-term primary productionl dataset. This dataset replaces all previous versions of ST_PP_Iwaniec_001 original. The FCE program is discontinuing its practice of versioning data as of March 2013.