Dataset title: Florida Bay Stable Isotope Data Everglades National Park (FCE LTER), Florida, USA, September 2000 - ongoing Dataset ID: LT_PP_Fourqurean_003 Research type: Long-term Dataset Creator Name: Dr. James Fourqurean Position: Principal Investigator Organization: Florida Coastal Everglades LTER Program Address: Florida International University 11200 SW 8th Street, OE 148 Miami, FL 33199 USA Phone: 305-348-4084 Email: fourqure@fiu.edu URL: https://seagrass.fiu.edu Metadata Provider Organization: Florida Coastal Everglades LTER Address: Florida International University 11200 SW 8th Street OE 148 Miami, FL 33199 USA Phone: 305-348-6054 Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Dataset Abstract 13C and 15N stable isotope data from seagrasses collected during visits to FCE LTER core research sites TS/Ph7a, TS/Ph8, TS/Ph9, TS/Ph10 and TS/Ph11. Geographic Coverage Study Extent Description The Study Extent of this dataset includes the FCE Florida Bay research sites within Everglades National Park, South Florida Bounding Coordinates Geographic description: TS/Ph7a West bounding coordinate: -80.639 East bounding coordinate: -80.639 North bounding coordinate: 25.191 South bounding coordinate: 25.191 Geographic description: TS/Ph8 West bounding coordinate: -80.525 East bounding coordinate: -80.525 North bounding coordinate: 25.233 South bounding coordinate: 25.233 Geographic description: TS/Ph9 West bounding coordinate: -80.49 East bounding coordinate: -80.49 North bounding coordinate: 25.177 South bounding coordinate: 25.177 Geographic description: TS/Ph10 West bounding coordinate: -80.681 East bounding coordinate: -80.681 North bounding coordinate: 25.025 South bounding coordinate: 25.025 Geographic description: TS/Ph11 West bounding coordinate: -80.938 East bounding coordinate: -80.938 North bounding coordinate: 24.913 South bounding coordinate: 24.913 FCE LTER Sites: TS/Ph7a, TS/Ph8, TS/Ph9, TS/Ph10, TS/Ph11 Temporal Coverage Start Date: 2000-09-10 End Date: 2024-05-03 Data Table Entity Name: LT_PP_Fourqurean_003.csv Entity Description: Florida Bay Stable Isotope Data Everglades National Park (FCE LTER), South Florida Object Name: LT_PP_Fourqurean_003.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: SITENAME Attribute Label: sitename Attribute Definition: Name of LTER site Storage Type: Measurement Scale: text Missing Value Code: Attribute Name: SITEALIAS Attribute Label: alias Attribute Definition: Alias of LTER site Storage Type: Measurement Scale: text Missing Value Code: Attribute Name: SiteSerialNum Attribute Label: ID number Attribute Definition: Site ID number Storage Type: Measurement Scale: text Missing Value Code: Attribute Name: Date Attribute Label: date Attribute Definition: Date of collection Storage Type: Measurement Scale: Missing Value Code: Attribute Name: DecimalYear Attribute Label: decimal year Attribute Definition: Calendar year + fraction of calendar year Storage Type: Measurement Scale: text Missing Value Code: Attribute Name: Species_Seagrass Attribute Label: species Attribute Definition: Species Seagrass Storage Type: Measurement Scale: text Missing Value Code: -9999 (Value will never be recorded) Attribute Name: delta_15N Attribute Label: isotope Attribute Definition: Delta 15N Storage Type: Measurement Scale: Units: perMil Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded) Attribute Name: delta_13C Attribute Label: isotope Attribute Definition: Delta 13 Storage Type: Measurement Scale: Units: perMil Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded) Methods Sampling Description At each sampling site, 5 intact short shoots of Thalassia testudinum (and a minimum of 10 Syringodium filiforme and 15 Halodule wrightii short shoots, if present) were haphazardly collected from a 10 m2 area. These short shoots were returned to the lab, where all attached green leaves were cut from the short shoots and cleaned of adhering epiphytes by gently scraping with a razor blade. All leaves from a site were pooled and dried at 80degrees C. Dried leaves were ground to a fine powder using a ceramic mortar and pestle. Powdered samples were analyzed for 15N and 13C at the SERC Stable Isotope Laboratory at Florida International University using standard elemental analyzer isotope ratio mass spectrometer (EA-IRMS) procedures. Standards are Peedee Belemnite (PDB) for carbon and air (AIR) for nitrogen. Method Step Description At each sampling site, 5 intact short shoots of Thalassia testudinum (and a minimum of 10 Syringodium filiforme and 15 Halodule wrightii short shoots, if present) were haphazardly collected from a 10 m2 area. These short shoots were returned to the lab, where all attached green leaves were cut from the short shoots and cleaned of adhering epiphytes by gently scraping with a razor blade. All leaves from a site were pooled and dried at 80 degrees C. Dried leaves were ground to a fine powder using a ceramic mortar and pestle.Powdered samples were analyzed for 15N and 13C at the SERC Stable Isotope Laboratory at Florida International University using standard elemental analyzer isotope ratio mass spectrometer (EA-IRMS) procedures. Standards are Peedee Belemnite (PDB) for carbon and air (AIR) for nitrogen. Instrumentation Standard elemental analyzer isotope ratio mass spectrometer (EA-IRMS) Quality Control Data is entered and then checked for entry errors. All data is checked against field logs for entry errors. Distribution Online distribution: https://pasta.lternet.edu/package/data/eml/knb-lter-fce/1131/10/74572504febd748386a9ab6d6230c9cb Intellectual Rights This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you. Dataset Keywords FCE Florida Coastal Everglades LTER FCE LTER ecological research long-term monitoring Florida Bay Everglades National Park stable isotopes isotopes Halodule wrightii Syringodium filiforme Thalassia testudinum Shoalgrass Manatee grass Turtle grass delta 13C delta 15N seagrass species processes leaves nitrogen-15 freshwater estuarine nutrients water long term monitoring stable isotopes estuaries primary production primary production Maintenance knb-lter-fce.1131.10: Added new data, updated values for five July 2022 samples after they were re-run, updated metadata Dataset Contact Name: Jim Fourqurean Position: Principal Investigator Organization: Florida Coastal Everglades LTER Program Address: Florida International University 11200 SW 8th Street, OE 148 Miami, FL 33199 USA Phone: 305-348-4084 Email: fourqure@fiu.edu URL: https://seagrass.fiu.edu/ Position: Information Manager Organization: Florida Coastal Everglades LTER Address: Florida International University 11200 SW 8th Street Miami, FL 33199 USA Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Project permits EVER-2001-SCI-0071 EVER-2002-SCI-0070 EVER-2003-SCI-0067 EVER-2004-SCI-0070 EVER-2005-SCI-0081 EVER-2007-SCI-0065 EVER-2009-SCI-0070 EVER-2011-SCI-0071 EVER-2013-SCI-0058 EVER-2015-SCI-0062 EVER-2017-SCI-0054 EVER-2019-SCI-0055 EVER-2022-SCI-0029 EVER-2023-SCI-0011 EVER-2024-SCI-0011 Information Management Notes This is a long-term primary production dataset and subsequent data will be appended. This dataset replaces all previous versions of LT_PP_Fourqurean_004, LT_PP_Fourqurean_014 and LT_PP_Fourqurean_015 as the dataset were combined into LT_PP_Fourqurean_003 to facilitate data usability. The FCE program is discontinuing its practice of versioning data as of March 2013.