Dataset title: Periphyton Biomass Accumulation from the Shark River and Taylor Sloughs, Everglades National Park (FCE LTER), South Florida, USA, January 2003 - ongoing Dataset ID: doi:10.6073/pasta/fda2abf8c0c081843917328deeeab17a Research type: Long-term Dataset Creator Name: Dr. Evelyn Gaiser Organization: Florida Coastal Everglades LTER Address: 11200 S.W. 8th Street Miami, Florida 33199 USA Phone: 305-348-6145 Email: gaisere@fiu.edu URL: http://algae.fiu.edu Name: Franco Tobias Organization: Florida Coastal Everglades LTER Address: Florida International University 11200 SW 8th Street, OE 148 Miami, FL 33199 USA Phone: 305-348-3095 Email: tobiasf@fiu.edu Metadata Provider Organization: Florida Coastal Everglades LTER Address: Florida International University 11200 SW 8th Street, OE 148 Miami, FL 33199 USA Phone: 305-348-6054 Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Dataset Abstract Periphyton biomass was measured quarterly at three replicate locations (1, 2, and 3) at FCE LTER marsh sites (SRS 1-3; TS/Ph 1-3). A 1-m2 plot was placed in the marsh and percent cover of periphyton was visually estimated, including cover on the water surface (floating), plant stems (epiphytic), and on the bottom (benthic). Periphyton was then harvested from the quadrats into a 2000 ml perforated graduated cylinder and the volume measured. A 120-ml subsample was removed and taken back to the laboratory for analysis. Data provided include percent cover by substrate, dry, ash, and ash-free dry mass per area, chlorophyll a per gram ash-free dry mass and per area, and the total phosphorus, nitrogen and carbon concentration on a dry weight basis. This is part of a continuous data collection to test the hypothesis that phosphorus and hydrology interact to influence periphyton abundance in Everglades marshes. Geographic Coverage Study Extent Description The Study Extent of this dataset includes the FCE Shark River and Taylor Slough research sites within Everglades National Park, South Florida Bounding Coordinates Geographic description: SRS1b West bounding coordinate: -80.726 East bounding coordinate: -80.726 North bounding coordinate: 25.758 South bounding coordinate: 25.758 Geographic description: SRS1c West bounding coordinate: -80.57 East bounding coordinate: -80.57 North bounding coordinate: 25.75 South bounding coordinate: 25.75 Geographic description: SRS1d West bounding coordinate: -80.65 East bounding coordinate: -80.65 North bounding coordinate: 25.75 South bounding coordinate: 25.75 Geographic description: SRS2 West bounding coordinate: -80.785 East bounding coordinate: -80.785 North bounding coordinate: 25.55 South bounding coordinate: 25.55 Geographic description: SRS3 West bounding coordinate: -80.853 East bounding coordinate: -80.853 North bounding coordinate: 25.468 South bounding coordinate: 25.468 Geographic description: TS/Ph1b West bounding coordinate: -80.59 East bounding coordinate: -80.59 North bounding coordinate: 25.439 South bounding coordinate: 25.439 Geographic description: TS/Ph2 West bounding coordinate: -80.607 East bounding coordinate: -80.607 North bounding coordinate: 25.404 South bounding coordinate: 25.404 Geographic description: TS/Ph3 West bounding coordinate: -80.663 East bounding coordinate: -80.663 North bounding coordinate: 25.252 South bounding coordinate: 25.252 FCE LTER Sites: SRS1a, SRS2, SRS3, SRS1c, SRS1d, TS/Ph2, TS/Ph3, TS/Ph1b Temporal Coverage Start Date: 2003-01-06 End Date: 2022-10-22 Data Table Entity Name: LT_PP_Gaiser_005.csv Entity Description: Periphyton Biomass Accumulation from the Shark River and Taylor Slough, Everglades National Park, from January 2003 to October present Object Name: LT_PP_Gaiser_005.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: SITENAME Attribute Label: sitename Attribute Definition: Name of LTER site Storage Type: string Measurement Scale: SRS1b= FCE LTER site SRS1b SRS1c= FCE LTER site SRS1c SRS1d= FCE LTER site SRS1d SRS2= FCE LTER site SRS2 SRS3= FCE LTER site SRS3 TS/Ph1b= FCE LTER site TS/Ph1b TS/Ph2= FCE LTER site TS/Ph2 TS/Ph3= FCE LTER site TS/Ph3 Missing Value Code: Attribute Name: Substrate Attribute Label: substrate Attribute Definition: Substrate-Periphytometer Storage Type: text Measurement Scale: M= Mats P= periphytometer E= Epiphyton Missing Value Code: Attribute Name: Replicate Attribute Label: ID Number Attribute Definition: Replicate ID Number Storage Type: ordinal Measurement Scale: text Missing Value Code: Attribute Name: Date Attribute Label: date Attribute Definition: Collection date Storage Type: datetime Measurement Scale: Missing Value Code: Attribute Name: Areal_Mat_Coverage Attribute Label: proportion Attribute Definition: Proportion of periphyton cover Storage Type: data Measurement Scale: Units: percentPerMeterSquared Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded) Attribute Name: Floating_Periphyton_Cover Attribute Label: percent cover Attribute Definition: Areal Mat Coverage Storage Type: data Measurement Scale: Units: percentPerMeterSquared Precision: 1 Number Type: real Missing Value Code: -9999 (Value will never be recorded) Attribute Name: Benthic_Periphyton_Cover Attribute Label: percent cover Attribute Definition: Areal Mat Coverage Storage Type: data Measurement Scale: Units: percentPerMeterSquared Precision: 1 Number Type: real Missing Value Code: -9999 (Value will never be recorded) Attribute Name: Epiphytic_Periphyton_Cover Attribute Label: percent cover Attribute Definition: Areal Mat Coverage Storage Type: data Measurement Scale: Units: percentPerMeterSquared Precision: 1 Number Type: real Missing Value Code: -9999 (Value will never be recorded) Attribute Name: DryWeight Attribute Label: weight Attribute Definition: Areal Dry Weight Storage Type: data Measurement Scale: Units: gramsPerSquareMeter Precision: 0.0001 Number Type: real Missing Value Code: -9999.0000 (Value will never be recorded) Attribute Name: AshWeight Attribute Label: weight Attribute Definition: Areal Ash Weight Storage Type: data Measurement Scale: Units: gramsPerSquareMeter Precision: 0.0001 Number Type: real Missing Value Code: -9999.0000 (Value will never be recorded) Attribute Name: AFDM Attribute Label: mass Attribute Definition: Ash Free Dry Weight Accumulation Storage Type: data Measurement Scale: Units: gramsPerSquareMeter Precision: 0.0001 Number Type: real Missing Value Code: -9999.0000 (Value will never be recorded) Attribute Name: TP Attribute Label: concentration Attribute Definition: Total Phosphorus Storage Type: data Measurement Scale: Units: microgramsPerGram Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded) Attribute Name: Percent_TN Attribute Label: percent content Attribute Definition: Total nitrogen concentration in % dry weight Storage Type: data Measurement Scale: Units: percent Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded) Attribute Name: Percent_TC Attribute Label: concentration Attribute Definition: Total carbon concentration in % dry weight Storage Type: data Measurement Scale: Units: percent Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded) Attribute Name: CHLA_Content_Mat Attribute Label: concentration Attribute Definition: Chlorophyll a content of periphyton per area Storage Type: float Measurement Scale: Units: microgramsPerMeterSquared Precision: 1 Number Type: integer Missing Value Code: -9999 (Value will never be recorded) Attribute Name: CHLA_Content_AFDM Attribute Label: concentration Attribute Definition: Chlorophyll a content of Ash Free Dry Mass Storage Type: float Measurement Scale: Units: microgramsPerGram Precision: 1 Number Type: integer Missing Value Code: -9999 (Value will never be recorded) Methods Sampling Description Three random throws of the 1m square quadrat are made at each sampling site. Site, water and vegetation characteristics are recorded. Periphyton composition and volume are also recorded, and representative volume is brought back to the lab for processing and analysis Method Step Description Periphyton was characterized, measured and collected from three random throws in each LTER location. Periphyton was picked through for associated macrophytes and homogenized in distilled water. Subsamples were taken for measurements of dry mass, ash-free dry mass, chlorophyll a, species composition and total nutrient (C, N, P) content. References: Frankovich, T. A., & Fourqurean, J. W. (1997). Seagrass epiphyte loads along a nutrient availability gradient, Florida Bay, USA. Marine Ecology Progress Series, 159, 37-50. Welschmeyer, N. A. (1994). Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments. Limnology and oceanography, 39(8), 1985-1992. Childers, D. L., Jones, R. D., Trexler, J., Buzzelli, C., Dailey, S., Edwards, A. L., ... & Gelder, W. V. (2001). Quantifying the effects of low-level phosphorus enrichment on unimpacted Everglades wetlands with in situ flumes and phosphorus dosing. The Everglades, Florida Bay, and Coral Reefs of the Florida Keys. CRC Press, Boca Raton, FL, USA, 127-152. Instrumentation 1m square PVC quadrat, 2000mL graduated cylinder, sampling bags Quality Control Data entered and rechecked. Distribution Online distribution: https://pasta.lternet.edu/package/data/eml/knb-lter-fce/1111/14/655aa58991381b83043e038664197472 Intellectual Rights This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you. Dataset Keywords periphyton aquatic ecosystems primary production nutrients periphyton aquatic ecosystems primary production nutrients periphyton aquatic ecosystems primary production nutrients periphyton aquatic ecosystems primary production nutrients FCE FCE LTER Florida Coastal Everglades LTER ecological research long-term monitoring productivity water Tamiami Trail bridge canal macrophytes plants phosphorus accumulation FCE FCE LTER Florida Coastal Everglades LTER ecological research long-term monitoring productivity water Tamiami Trail bridge canal macrophytes plants phosphorus accumulation FCE FCE LTER Florida Coastal Everglades LTER ecological research long-term monitoring productivity water Tamiami Trail bridge canal macrophytes plants phosphorus accumulation FCE FCE LTER Florida Coastal Everglades LTER ecological research long-term monitoring productivity water Tamiami Trail bridge canal macrophytes plants phosphorus accumulation Data Submission Date: 2022-03-10 Maintenance 1111.14: Added new data, updated metadata Dataset Contact Position: Information Manager Organization: Florida Coastal Everglades LTER Address: Florida International University 11200 SW 8th Street, OE 148 Miami, FL 33199 USA Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Project permits EVER-2019-SCI-0055 Dataset Submission Date 2022-03-10 Data Submission Notes Sample mass accumulation rate = (sample vol/subsample vol * subsample weight after drying or ashing)/area of substrate/incubation time. Substrate type (P=periphytometer; M=mat; E=Epiphyton). Blanks cells may have values entered in subsequent data submissions.