Dataset title: Periphyton Accumulation Rates from Shark River Slough, Taylor Slough and Florida Bay, Everglades National Park (FCE LTER) from January 2001 to Present Dataset ID: LT_PP_Gaiser_003 Research type: Long-term Dataset Creator Name: Dr. Evelyn Gaiser Position: Primary Investigator Organization: Florida Coastal Everglades LTER Program Address: 11200 S.W. 8th Street Miami, Florida 33199 USA Phone: 305-348-6145 Fax: 305-348-4096 Email: gaisere@fiu.edu URL: http://serc.fiu.edu/periphyton/ Metadata Provider Organization: Florida Coastal Everglades LTER Program Address: Florida International University University Park OE 148 Miami, FL 33199 USA Phone: 305-348-6054 Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Dataset Abstract Periphyton accumulation rates were measured bimonthly at FCE LTER sites in Florida Bay and Shark Slough. Glass slides were used as artificial substrates for periphyton growth. Twenty slides were incubated for 2 months in triplicate periphytometers (flow-through plastic boxes) at the surface (SRS sites) and bottom (FB) at each site. Twelve artificial blades (made from transparency film) were incubated for 2 months in triplicate in specially constructed wire frames at the bottom at sites TS/PH 9, TS/PH 10 and TS/PH 11. Data are presented in terms of grams dry and ash-free dry mass per area per time. This is part of continuous data collection to test the hypothesis that phosphorus and nitrogen limit productivity at the upstream and downstream ends, respectively, of the FCE transects. Conclusions await longer-term data collection. Geographic Coverage Study Extent Description The Study Extent of this dataset includes the FCE Shark River Slough and Florida Bay research sites within Everglades National Park, South Florida Bounding Coordinates Geographic description: FCE LTER Site SRS1a West bounding coordinate: -80.727 East bounding coordinate: -80.727 North bounding coordinate: 25.761 South bounding coordinate: 25.761 Geographic description: FCE LTER Site SRS2 West bounding coordinate: -80.785 East bounding coordinate: -80.785 North bounding coordinate: 25.550 South bounding coordinate: 25.550 Geographic description: FCE LTER Site SRS3 West bounding coordinate: -80.853 East bounding coordinate: -80.853 North bounding coordinate: 25.468 South bounding coordinate: 25.468 Geographic description: FCE LTER Site TS/Ph4 West bounding coordinate: -80.522 East bounding coordinate: -80.522 North bounding coordinate: 25.315 South bounding coordinate: 25.315 Geographic description: FCE LTER Site TS/Ph5 West bounding coordinate: -80.520 East bounding coordinate: -80.520 North bounding coordinate: 25.295 South bounding coordinate: 25.295 Geographic description: FCE LTER Site TS/Ph9 West bounding coordinate: -80.490 East bounding coordinate: -80.490 North bounding coordinate: 25.177 South bounding coordinate: 25.177 Geographic description: FCE LTER Site TS/Ph10 West bounding coordinate: -80.681 East bounding coordinate: -80.681 North bounding coordinate: 25.025 South bounding coordinate: 25.025 Geographic description: FCE LTER Site TS/Ph11 West bounding coordinate: -80.938 East bounding coordinate: -80.938 North bounding coordinate: 24.913 South bounding coordinate: 24.913 Geographic description: FCE LTER Site SRS1c West bounding coordinate: -80.57 East bounding coordinate: -80.57 North bounding coordinate: 25.75 South bounding coordinate: 25.75 Geographic description: FCE LTER Site SRS1d West bounding coordinate: -80.65 East bounding coordinate: -80.65 North bounding coordinate: 25.75 South bounding coordinate: 25.75 Geographic description: FCE LTER Site TS/Ph1b West bounding coordinate: -80.590 East bounding coordinate: -80.590 North bounding coordinate: 25.439 South bounding coordinate: 25.439 Geographic description: FCE LTER Site TS/Ph2 West bounding coordinate: -80.607 East bounding coordinate: -80.607 North bounding coordinate: 25.404 South bounding coordinate: 25.404 Geographic description: FCE LTER Site TS/Ph3 West bounding coordinate: -80.663 East bounding coordinate: -80.663 North bounding coordinate: 25.252 South bounding coordinate: 25.252 FCE LTER Sites: SRS1a, SRS1c, SRS1d, SRS2, SRS3, TS/Ph4, TS/Ph5, TS/Ph9, TS/Ph10, TS/Ph11,TS/Ph1b,TS/Ph2,TS/Ph3 All Sites Geographic Description:FCE LTER Site SRS1a Longitude:-80.727 Latitude:25.761 Geographic Description:FCE LTER Site SRS2 Longitude:-80.785 Latitude:25.550 Geographic Description:FCE LTER Site SRS3 Longitude:-80.853 Latitude:25.468 Geographic Description:FCE LTER Site TS/Ph4 Longitude:-80.522 Latitude:25.315 Geographic Description:FCE LTER Site TS/Ph5 Longitude:-80.520 Latitude:25.295 Geographic Description:FCE LTER Site TS/Ph9 Longitude:-80.490 Latitude:25.177 Geographic Description:FCE LTER Site TS/Ph10 Longitude:-80.681 Latitude:25.025 Geographic Description:FCE LTER Site TS/Ph11 Longitude:-80.938 Latitude:24.913 Geographic Description:FCE LTER Site SRS1c Longitude:-80.57 Latitude:25.75 Geographic Description:FCE LTER Site SRS1d Longitude:-80.65 Latitude:25.75 Geographic Description:FCE LTER Site TS/Ph1b Longitude:-80.590 Latitude:25.439 Geographic Description:FCE LTER Site TS/Ph2 Longitude:-80.607 Latitude:25.404 Geographic Description:FCE LTER Site TS/Ph3 Longitude:-80.663 Latitude:25.252 Temporal Coverage Start Date: 2001-01-06 End Date: 2019-10-23 Data Table Entity Name: LT_PP_Gaiser_003.txt Entity Description: Periphyton Accumulation Rates from the Shark River Slough and Taylor Slough, Everglades National Park Object Name: LT_PP_Gaiser_003.txt Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: Transect Attribute Label: Transect Attribute Definition: Location along freshwater-saline gradient Storage Type: text Measurement Scale: SRS = Shark River Slough; TS Missing Value Code: Attribute Name: Substrate Attribute Label: substrate Attribute Definition: Substrate-Periphytometers Storage Type: text Measurement Scale: Substrate-Periphytometers Missing Value Code: Attribute Name: Replicate Attribute Label: ID Number Attribute Definition: Replicate ID Number Storage Type: ordinal Measurement Scale: Replicate ID Number Missing Value Code: Attribute Name: Date Attribute Label: date Attribute Definition: Collection date Storage Type: datetime Measurement Scale: Missing Value Code: Attribute Name: IncubationTime Attribute Label: time Attribute Definition: Incubation Time Storage Type: nominal Measurement Scale: Incubation Time Missing Value Code: Attribute Name: DryWeight Attribute Label: weight Attribute Definition: Dry Weight Production Storage Type: data Measurement Scale: Units: gramsPerMeterSquaredPerDay Precision: 0.0001 Number Type: real Missing Value Code: -9999.0000 (Value will never be recorded ) Attribute Name: AFDM Attribute Label: weight Attribute Definition: Ash Free Dry Mass accumulation Storage Type: data Measurement Scale: Units: gramsPerMeterSquaredPerDay Precision: 0.0001 Number Type: real Missing Value Code: -9999.0000 (Value will never be recorded ) Attribute Name: ORG Attribute Label: percent Attribute Definition: Percent total organic content Storage Type: data Measurement Scale: Units: percent Precision: 1 Number Type: real Missing Value Code: -9999 (Value will never be recorded) Attribute Name: TP Attribute Label: concentration Attribute Definition: Total Phosphorus Storage Type: data Measurement Scale: Units: microgramsPerGram Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded ) Methods Sampling Description Accumulation rates are measured quarterly when water is present. Triplicate periphytometers, each containing 20 slides, were incubated at the water surface (SRS sites) and on top of the sediments (FB sites); artificial seagrass blades were incubated at Florida Bay sites. Periphytometers were placed near the markers for each site. Method Step Description Periphytometers and artificial blades were collected after 2 months of incubation and returned on ice to the lab. Periphyton was scraped from slides into beakers and homogenized in distilled water. Subsamples were taken for measurements of dry mass, ash-free dry mass, chlorophyll a, species composition and total nutrient (C, N, P) content. Citation Frankovich, T. A. , J W Fourqurean. 1997. Seagrass epiphyte loads along a nutrient availablitity gradient, Florida Bay, USA.. Marine Ecology Progress Series, 159: 37-50. Instrumentation Artificial substrates described as glass slides, mylar blades Method Step Description Periphytometers and artificial blades were collected after 2 months of incubation and returned on ice to the lab. Periphyton was scraped from slides into beakers and homogenized in distilled water. Subsamples were taken for measurements of dry mass, ash-free dry mass, chlorophyll a, species composition and total nutrient (C, N, P) content. Citation Welschmeyer, N A 1994. Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments. Limnology and Oceanography, 39(8): 1985-1992. Instrumentation Artificial substrates described as glass slides, mylar blades Method Step Description Periphytometers and artificial blades were collected after 2 months of incubation and returned on ice to the lab. Periphyton was scraped from slides into beakers and homogenized in distilled water. Subsamples were taken for measurements of dry mass, ash-free dry mass, chlorophyll a, species composition and total nutrient (C, N, P) content. Citation Childers, D. L. , R D Jones, J C Trexler, C Buzzelli, J Boyer, A L Edwards, E E Gaiser, K Jayachandaran, D Lee, J F Meeder, J Pechmann, J H Richards, L J Scinto. 2001. Quantifying the effects of low level phosphorus enrichment on unimpacted Everglades wetlands with in situ flumes and phosphorus dosing. p. 127-152 in Porter, J , K Porter. , (eds). The Everglades, Florida Bay, and Coral Reefs of the Florida Keys. CRC Press, Boca Raton, FL, USA, Instrumentation Artificial substrates described as glass slides, mylar blades Quality Control Field data are written in pencil on waterproof paper and photocopied upon return to the laboratory. They are then entered into the appropriate digital format. These data are reviewed in hard copy and digital format by the lab manager. Laboratory data are written in pencil on waterproof paper, photocopied prior to computer entry and then entered digitally in duplicate and cross-referenced to hard copy notes. Data outliers, determined by comparison to long-term variance in that parameter, are highlighted in resulting spreadsheets for examination by the principal investigator, who may discard the values. Distribution Online distribution: https://fce-lter.fiu.edu/perl/public_data_download.pl?datasetid=LT_PP_Gaiser_003.txt Intellectual Rights This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you. Dataset Keywords primary production aquatic ecosystems periphyton net primary production FCE Florida Coastal Everglades LTER FCE LTER ecological research long-term monitoring productivity primary production periphyton water Tamiami Trail grand experiment bridge canal macrophytes plants nutrients Total Phosphorus TP percent total organic content Data Submission Date: 2021-02-03 Maintenance This is a long-term primary production dataset and subsequent data wil be appended. This dataset replaces all previous versions of LT_PP_Gaiser_003 original. The FCE program is discontinuing its practice of versioning data as of March 2013. Dataset Contact Position: Information Manager Organization: Florida Coastal Everglades LTER Program Address: Florida International University University Park OE 148 Miami, FL 33199 USA Phone: 305-348-6054 Fax: 305-348-4096 Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Project permits EVER-2019-SCI-0055 Dataset Submission Date 2021-02-03 Data Submission Notes Please Note that the data file LT_PP_Gaiser_003 is replacing previous data file ST_PP_Gaiser_001 (all versions). Sample mass accumulation rate = (sample vol/subsample vol x subsample weight after drying or ashing)/area of substrate/incubation time. P = periphytometers. Blanks indicate that data may be forthcoming at a later date. Information Management Notes Information Manager (December 19, 2011) 1) The file LT_PP_Gaiser_003 should NOT include %n and %C and SHOULD include the Organic Content (ORG) column. This new file replaces the old data files (ST_PP_Gaiser_001 & all versions) so that these values are all updated. Dr. Gaiser decided to drop the N and C because they often don't have enough material to reliably analyze that, and the Organic Content shows interesting patterns that are important to report, 2) Changed date format to DD-MMM-YY and coded missing values with -9999 (including changing NES and NS to -9999), 3) Reordered and renamed columns to SITENAME, Date, Substrate, Replicate, IncubationTime, DryWeight, AFDM, ORG and TP, 4) Corrected site names to reflect TS/Ph instead of TSPH. This is a long-term primary production dataset and subsequent data wil be appended. This dataset replaces all previous versions of LT_PP_Gaiser_003 original. The FCE program is discontinuing its practice of versioning data as of March 2013.