Dataset title: Surface Water Quality Monitoring Data collected in South Florida Coastal Waters (FCE LTER), Florida, USA, June 1989-ongoing Dataset ID: LT_ND_Boyer_001 Research type: Long-Term Monitoring Dataset Creator Name: Dr. Henry Briceno Address: Florida International University 11200 SW 8th Street OE 148 Miami, FL 33199 USA Phone: 305-348-1269 Fax: 305-348-4096 Email: bricenoh@fiu.edu Metadata Provider Organization: Florida Coastal Everglades LTER Address: Florida International University 11200 SW 8th Street OE 148 Miami, FL 33199 USA Phone: 305-348-6054 Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Organization: Southeast Environmental Research Center Address: Florida International University University Park OE 148 Miami, FL 33199 USA Phone: 305-348-4076 Email: boyerj@fiu.edu URL: http://serc.fiu.edu/wqmnetwork/ Dataset Abstract The Southeast Environmental Research Center at Florida International University operates a network of 331 fixed sampling sites distributed throughout the estuarine and coastal ecosystems of south Florida. The purpose of this network is to address concerns in regional water quality which cross and overlap separate political boundaries. Funding has come from different sources with individual programs being added as funding became available. Biscayne Bay, Florida Bay, Whitewater Bay, Ten Thousand Islands, Rookery Bay, Estero Bay, and Pine Island Sound are sampled monthly while the Florida Keys National Marine Sanctuary (FKNMS) and the southwest shelf are sampled quarterly. Variables currently being measured include surface and bottom temperature, salinity, dissolved oxygen, nitrate, nitrite, ammonium, total nitrogen, total organic nitrogen, total phosphorus, soluble reactive phosphorus, total organic carbon, total silicate, chlorophyll a, alkaline phosphatase activity, turbidity, and light extinction. The purpose of this network is to address concerns in regional water quality which cross and overlap separate political boundaries. One of the products is a quasi-synoptic big picture of nutrient and phytoplankton biomass distributions over the South Florida Coastal Waters. The SERC network will, in time, provide us with the data necessary to determine whether conditions within the estuaries and sanctuary are improving or declining. Geographic Coverage Study Extent Description South Florida Coastal Waters Including Florida Bay and Whitewater Bay. Bounding Coordinates Geographic description: TS/Ph9 West bounding coordinate: -80.490 East bounding coordinate: -80.490 North bounding coordinate: 25.177 South bounding coordinate: 25.177 Geographic description: TS/Ph10 West bounding coordinate: -80.681 East bounding coordinate: -80.681 North bounding coordinate: 25.025 South bounding coordinate: 25.025 Geographic description: TS/Ph11 West bounding coordinate: -80.938 East bounding coordinate: -80.938 North bounding coordinate: 24.913 South bounding coordinate: 24.913 Geographic description: Joe Bay West bounding coordinate: -80.537 East bounding coordinate: -80.537 North bounding coordinate: 25.224 South bounding coordinate: 25.224 Geographic description: Little Madeira Bay West bounding coordinate: -80.627 East bounding coordinate: -80.627 North bounding coordinate: 25.175 South bounding coordinate: 25.175 Geographic description: Tarpon Bay West bounding coordinate: -80.998 East bounding coordinate: -80.998 North bounding coordinate: 25.417 South bounding coordinate: 25.417 Geographic description: Gunboat Island West bounding coordinate: -81.031 East bounding coordinate: -81.031 North bounding coordinate: 25.379 South bounding coordinate: 25.379 Geographic description: Ponce de Leon Bay West bounding coordinate: -81.125 East bounding coordinate: -81.125 North bounding coordinate: 25.350 South bounding coordinate: 25.350 Geographic description: Oyster Bay West bounding coordinate: -81.073 East bounding coordinate: -81.073 North bounding coordinate: 25.331 South bounding coordinate: 25.331 FCE LTER Sites: TS/Ph9,TS/Ph10, TS/Ph11, Joe Bay-WQM Station 10, Little Madeira Bay-WQM Station 11, Tarpon Bay-WQM Station 38, Gunboat Island-WQM Station 39, Ponce De Leon Bay-WQM Station 40, Oyster Bay-WQM Station 41 Temporal Coverage Start Date: 1989-06-27 End Date: 2022-12-08 Data Table Entity Name: LT_ND_Boyer_001.csv Entity Description: Surface Water Quality Monitoring Data collected in South Florida Coastal Waters (FCE) Object Name: LT_ND_Boyer_001.csv Data Format Number of Header Lines: 1 Attribute Orientation: column Field Delimiter: , Number of Records: Attributes Attribute Name: SURV Attribute Label: Survey Number Attribute Definition: Survey Number Storage Type: ordinal Measurement Scale: Survey Number Missing Value Code: Attribute Name: Date Attribute Label: date Attribute Definition: Date of Sampling Storage Type: datetime Measurement Scale: Missing Value Code: -9999 (not collected) Attribute Name: TIME Attribute Label: Time Attribute Definition: Time of Sample Collection Storage Type: Measurement Scale: Missing Value Code: -9999 (not collected) Attribute Name: STA Attribute Label: Station ID Attribute Definition: WQM Station ID where samples collected Storage Type: ordinal Measurement Scale: WQM Station ID where samples collected Missing Value Code: Attribute Name: SITE Attribute Label: Collection Site Attribute Definition: Name of station collection site Storage Type: text Measurement Scale: Name of station collection site Missing Value Code: Attribute Name: LATDEC Attribute Label: LATDEC Attribute Definition: Decimal Latitude Storage Type: coordinate Measurement Scale: Units degree Precision:0.00001 Number Type: real Missing Value Code: Attribute Name: LONDEC Attribute Label: LONDEC Attribute Definition: Decimal Longitude Storage Type: coordinate Measurement Scale: Units degree Precision:0.00001 Number Type: real Missing Value Code: Attribute Name: DEPTH Attribute Label: DEPTH Attribute Definition: Station Depth Storage Type: data Measurement Scale: Units meter Precision:0.1 Number Type: real Missing Value Code: Attribute Name: BAY Attribute Label: Bay Attribute Definition: Accronym of Bay where samples are collected Storage Type: text Measurement Scale: FB= Florida Bay WWB= White Water Bay Missing Value Code: Attribute Name: NOX Attribute Label: NOX Attribute Definition: Nitrate + Nitrite Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: NO3 Attribute Label: NO3 Attribute Definition: Nitrate Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: NO2 Attribute Label: NO2 Attribute Definition: Nitrite Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: NH4 Attribute Label: NH4 Attribute Definition: Ammonium Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: TN Attribute Label: Total Nitrogen Attribute Definition: Total Nitrogen Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: DIN Attribute Label: DIN Attribute Definition: Dissolved Inorganic Nitrogen Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: TON Attribute Label: TON Attribute Definition: Total Organic Nitrogen Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: TP Attribute Label: Total Phosphorus Attribute Definition: Total Phosphorus Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: SRP Attribute Label: SRP Attribute Definition: Soluble Reactive Phosphorus Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: APA Attribute Label: APA Attribute Definition: Alkaline Phosphatase Activity Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: CHLA Attribute Label: CHLA Attribute Definition: Chlorophyll a Storage Type: data Measurement Scale: Units: microgramsPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: TOC Attribute Label: TOC Attribute Definition: Total Organic Carbon Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: SiO2 Attribute Label: SiO2 Attribute Definition: Silicon Dioxide in Water Storage Type: data Measurement Scale: Units: microMolesPerLiter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: SAL_S Attribute Label: Salinity Attribute Definition: Surface Salinity Storage Type: data Measurement Scale: Units: PSU Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded ) Attribute Name: SAL_B Attribute Label: Salinity Attribute Definition: Bottom Salinity Storage Type: data Measurement Scale: Units: PSU Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded ) Attribute Name: TEMP_S Attribute Label: Surface Water Temperature Attribute Definition: Surface Water Temperature Storage Type: data Measurement Scale: Units: celsius Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded ) Attribute Name: TEMP_B Attribute Label: Bottom Water Temperature Attribute Definition: Bottom Water Temperature Storage Type: data Measurement Scale: Units: celsius Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded ) Attribute Name: DO_S Attribute Label: Dissolved Oxygen at Surface Attribute Definition: Dissolved Oxygen at Surface Storage Type: data Measurement Scale: Units: milligramsPerLiter Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded ) Attribute Name: DO_B Attribute Label: Dissolved Oxygen at Bottom Attribute Definition: Dissolved Oxygen at Bottom Storage Type: data Measurement Scale: Units: milligramsPerLiter Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded ) Attribute Name: TURB Attribute Label: Turbidity Attribute Definition: Turbidity Storage Type: data Measurement Scale: Units: NTU Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: pH Attribute Label: pH Attribute Definition: pH Storage Type: data Measurement Scale: Units: dimensionless Precision: 0.01 Number Type: real Missing Value Code: -9999.00 (Value will never be recorded ) Attribute Name: Kd Attribute Label: Diffuse Light Attenuation Coefficient (m-1) Attribute Definition: Diffuse Light Attenuation Coefficient Storage Type: data Measurement Scale: Units: perMeter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: TN_TP Attribute Label: TN:TP Attribute Definition: Surface Total Nitrogen To Total Phosphorus Ratio Storage Type: data Measurement Scale: Units: dimensionless Precision: 0.1 Number Type: real Missing Value Code: -9999.0 (Value will never be recorded ) Attribute Name: N_P Attribute Label: N:P Attribute Definition: Surface Din To SRP Ratio Storage Type: data Measurement Scale: Units: dimensionless Precision: 0.1 Number Type: real Missing Value Code: -9999.0 (Value will never be recorded ) Attribute Name: DIN_TP Attribute Label: DIN:TP Attribute Definition: Dissolved Inorganic Nitrogen To Total Phosphorus Ratio Storage Type: data Measurement Scale: Units: dimensionless Precision: 0.1 Number Type: real Missing Value Code: -9999.0 (Value will never be recorded ) Attribute Name: DSIGT Attribute Label: DSIGT Attribute Definition: Delta Sigma-T Storage Type: data Measurement Scale: Units: kilogramPerCubicMeter Precision: 0.001 Number Type: real Missing Value Code: -9999.000 (Value will never be recorded ) Attribute Name: Si_DIN Attribute Label: Si:DIN Attribute Definition: Silicate to Dissolved Inorganic Nitrogen Ratio Storage Type: data Measurement Scale: Units: dimensionless Precision: 0.1 Number Type: real Missing Value Code: -9999.0 (Value will never be recorded ) Methods Sampling Description Water samples were collected and analyzed by SERC personnel using standardmethodology outlined in the Quality Assurance Project Plan (QAPP) with prior approval from SFWMD and FDEP. Method Step Description Water samples were collected and analyzed by SERC personnel using standard methodology outlined in the Quality Assurance Project Plan (QAPP) with prior approval from SFWMD and FDEP. Salinity and temperature (degrees C) were measured 10 cm below the surface and 10 cm above the bottom using a combination salinity-conductivity-temperature probe (Orion model 140). Surface and bottom dissolved oxygen (DO, mg l-1) was measured using an oxygen electrode (Orion model 840), corrected for both salinity and temperature. Duplicate, unfiltered water samples were collected from 10 cm below the surface using sample rinsed, 120 ml HDPE bottles and kept at ambient temperature in the dark during transport. Duplicate water samples for dissolved nutrient analysis were collected using sample rinsed 150 ml syringes. These samples were filtered by hand (25 mm glass fiber GF/F) into acetone-washed and sample rinsed 60 ml HDPE bottles, which were then capped and immediately placed on ice in the dark for transport. The wet filters, used for chlorophyll a analysis (CHLA), were placed in 2 ml plastic centrifuge tubes to which 1.5 ml of 90% acetone was added. They were then immediately capped and put into a dark bottle on ice for transport. Unfiltered water samples were analyzed for total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), alkaline phosphatase activity (APA), and turbidity (NTU). TOC was measured by direct injection onto hot platinum catalyst in a Shimadzu TOC-5000 after first acidifying to pH<2 and purging with CO2-free air. TN was measured using an ANTEK 7000N Nitrogen Analyzer using O2 as carrier gas instead of argon to promote complete recovery of the nitrogen in the water samples. TP was determined using a dry ashing, acid hydrolysis technique. The APA assay measures the activity of alkaline phosphatase, an enzyme used by bacteria to mineralize phosphate from organic compounds. The assay is performed by adding a known concentration of an organic phosphate compound (o-methylfluorescein phosphate) to an unfiltered water sample. Alkaline phosphatase in the water sample cleaves the phosphate, leaving o-methylfluorescein, a highly fluorescent compound. The fluorescence of initial and 2 hr incubations were measured using a Gilford Fluoro IV spectrofluorometer (excitation = 430 nm, emission = 507 nm) and subtracted to give APA (uM h-1). Turbidity was measured using an HF Scientific model DRT-15C turbidimeter and reported in NTU. Filtrates were analyzed for soluble reactive phosphorus (SRP), nitrate + nitrite (NOx), nitrite (NO2-), ammonium (NH4+), and silicate (Si(OH)4) by flow injection analysis (Alpkem model RFA 300). Filters for CHLA content (µg l-1) were allowed to extract for a minimum of 2 days at -20degrees C before analysis. Extracts were analyzed using a Gilford Fluoro IV Spectrofluorometer (excitation = 435 nm, emission = 667 nm) and compared to a standard curve of pure CHLA (Sigma). Some parameters were not measured directly, but were calculated by difference. Nitrate (NO3-) was calculated as NOX - NO2-. Dissolved inorganic nitrogen (DIN) was calculated as NOX + NH4+. Total organic nitrogen (TON) was defined as TN - DIN. Concentrations for all of these water quality variables are reported in units of milligrams per liter (mg l-1) or the equivalent parts per million (ppm), except where noted. All nutrient concentrations are based on the atomic weight of primary nutrient species (ppm-N, ppm-P, and ppm-C), not the molecular weight. All N:P ratios discussed are calculated on a molar basis. Citation Jones, R. D. , J. N. Boyer. 2002. Annual Report of the South Florida Estuarine Water Quality Monitoring Network, SFWMD/SERC Cooperative Agreement #C10244.. Southeast Environmental Research Center (SERC) and South Florida Water Management District, 67. Instrumentation HF Scientific model DRT-15C turbidimeter Gilford Fluoro IV spectrofluorometer Alpkem model RFA 300 Shimadzu TOC-5000 oxygen electrode (Orion model 840) ANTEK 7000N Nitrogen Analyzer Quality Control Quality Assurance Project Plan (QAPP) with prior approval from South Florida Water Management District (SFWMD) and Florida Department of Environmental Protection (FDEP). Distribution Online distribution: https://pasta.lternet.edu/package/data/eml/knb-lter-fce/1055/13/9a61989893f4da76c0a7558a867af50d Intellectual Rights This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you. Dataset Keywords inorganic nutrients FCE FCE LTER Florida Coastal Everglades LTER ecological research long-term monitoring aquatic freshwater marine nutrients organic phosphorus salinity nitrogen estuary saltwater surface water water chemistry water quality Florida Bay Whitewater Bay Everglades National Park Florida Data Submission Date: 2023-12-06 Maintenance This is a long-term water Quality dataset and subsequent data will be appended to this dataset. This dataset replaces all previous versions of LT_ND_Boyer_001.txt (v1-v4). The FCE program is discontinuing its practice of versioning data as of March 2013. Dataset Contact Name: Henry Briceno Position: Project Collaborator Organization: Southeast Environmental Research Center (SERC) at FIU Address: Florida International University 11200 SW 8th Street OE 148 Miami, FL 33199 USA Phone: 305-348-1269 Fax: 305-348-4096 Email: bricenoh@fiu.edu Position: Information Manager Organization: Florida Coastal Everglades LTER Address: Florida International University 11200 SW 8th Street OE 148 Miami, FL 33199 USA Email: fcelter@fiu.edu URL: http://fcelter.fiu.edu Dataset Submission Date 2023-12-06 Data Submission Notes This work was supported by the South Florida Water Management District (Contract #C10244) and older data are also available for download at http://www.fiu.edu/wqmnetwork. Information Management Notes These long-term water quality monitoring data are collected by Henry Briceno with the Southeast Environmental Research Center at FIU. Although some of these data were not collected using FCE LTER funding, the data are collected at or near the FCE LTER Research sites. These data are invaluable and are an important addition to the long-term water quality monitoring efforts being conducted by the FCE LTER. These data replace original LT_ND_Boyer_001 and all subsequent version prior to April 2013.