Relative Abundance Diatom Data from Periphyton Samples Collected for the Comprehensive Everglades Restoration Plan (CERP) Study (FCE) from February 2005 to November 2014
Authors: Evelyn Gaiser
Time period: 2005-02-05 to 2014-11-14
Package id: knb-lter-fce.1211.3
Dataset id: LT_CERP_Gaiser
How to cite:
Gaiser, E.. 2017. Relative Abundance Diatom Data from Periphyton Samples Collected for the Comprehensive Everglades Restoration Plan (CERP) Study (FCE) from February 2005 to November 2014. Environmental Data Initiative. https://doi.org/10.6073/pasta/cb0f7e88d28075a6ff1f59d008bb732c. Dataset accessed 2020-02-28.
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Dataset AbstractRelative abundance diatom data collected between February 2005 and November 2014
"The Comprehensive Everglades Restoration Plan (CERP) focuses on “getting the water right” in the south Florida ecosystem—getting the right amount of water of the right quality to the right places at the right time" (USACE & DoI, 2015. Central and Southern Florida Project Comprehensive Everglades Restoration Plan) in the Everglades ecosystems. To inform CERP, since February 2005 we have been investigating the spatio-temporal variations of distribution, biomass and diversity of algae (key aquatic primary producers) in periphyton mats in relation to hydrology, nutrients and pH, and other environmental conditions. This data set reports relative abundance data of the diatoms from this study.
Geographic CoverageStudy Extent Description
Between February 2005 and November 2014, samples were taken in the Everglades ecosystem, from LOX to ENP (NE to SW; see Dataset geographic description).
Data were collected in Principal Sampling Units (800m x 800m) in the following regions (see list of sites at row 121). LKO: Lake Okeechobee; LOX: the Arthur R. Marshall Loxahatchee National Wildlife Refuge (Water Conservation Area 1); Pal Mar (PAL); Pennsuco (PEN), Lostman’s Creek (LMC), the ‘Oligohaline’ area (OLG), Southern Marl Prairie (SMP), Shark River Slough (SRS) and Taylor Slough (TSL); Holey Land Wildlife Management Area (HOL), Water Conservation Areas (WCA) 2 and 3.
N: 27.096, S: 25.216, E: -80.150, W: -81.099
Temporal CoverageStart Date: 2005-02-05
End Date: 2014-11-14
Using generalized random-tessellation stratification, we chose a representative set of locations (800 m x 800 m principal sampling units, PSU), within which we sampled three sites in a habitat drawn from a pool of GPS coordinates. At sites where depth was < 1 m, and vegetation not too dense to hamper the formation of periphyton, and our movement in the field, we threw a 1 m3 enclosure, open both at the top and bottom, and collected samples of periphytic algae with a 120 mL plastic beaker from floating or benthic periphyton mats, depending on the depth. Where no mats were present, we took flocculent detritus from the benthos, as this also hosts algae; we then transported the samples back to the laboratory and froze them until analysis. We measured water depth (cm) with a metal ruler, and TP concentrations in the periphyton (μg g−1 dry weight) by means of colorimetry after dry combustion because water column concentrations are often below detection in this extremely oligotrophic wetland [Marazzi, L., E.E. Gaiser, V.J. Jones, F. Tobias, A.W. Mackay. 2017. Algal richness and life-history strategies are influenced by hydrology and phosphorus in two major subtropical wetlands. Freshwater Biology 62: 274-290. DOI: 10.1111/fwb.12866 ].
Diatom samples were cleaned of calcite and organic matter using strong acids and chemical oxidizers, and then permanently affixed to glass slides using Naphrax®. A minimum of 500 valves were counted and identified per slide using a compound light microscope at 1,000× magnification. Identifications were made to the lowest taxonomic level possible (variety or forma). [Lee et al. (2013) Diatom-based Models for Inferring Hydrology and Periphyton Abundance in a Subtropical Karstic Wetland: Implications for Ecosystem-Scale Bioassessment Wetlands 33: 157–173].
Distribution and Intellectual RightsOnline distribution
All publications based on this dataset must cite the data Contributor, the Florida Coastal Everglades Long-Term Ecological Research (LTER) Program and that this material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DEB-1237517, #DBI-0620409, and #DEB-9910514. Additionally, two copies of the manuscript must be submitted to the Florida Coastal Everglades LTER Program Office, LTER Program Manager, Florida International University, Southeast Environmental Research Center, OE 148, University Park, Miami, Florida 33199. For a complete description of the FCE LTER Data Access Policy and Data User Agreement, please go to FCE Data Management Policy at http://fcelter.fiu.edu/data/DataMgmt.pdf and LTER Network Data Access Policy at http://fcelter.fiu.edu/data/core/data_user_agreement/distribution_policy.html.
KeywordsComprehensive Everglades Restoration Plan (CERP), CERP, ecological research, long-term monitoring, restoration, algae, aquatic ecosystems, wetland, biomass
Data Table and FormatData Table: A wide matrix of Diatom Abundance vs. CERP sampling location
Data Table: A list of sampling locations from the CERP study using UTM coordinates