Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America from January 2012 to May 2014
At a Glance
Authors: Elizabeth Harrison, Joel Trexler
Time period: 2012-01-10 to 2014-05-30
Package id: knb-lter-fce.1201.3
Dataset id: ST_TDCS_Harrison_002
Harrison, E., J. Trexler. 2014. Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America from January 2012 to May 2014. Environmental Data Initiative. https://doi.org/10.6073/pasta/4a07f10ec6a08e78279a506423f22305. Dataset accessed 2024-12-04.
Geographic Coverage
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Dataset Creator(s)
- Name: Dr. Elizabeth Harrison
- Address: Georgia Gwinnet College
1000 University Center Lane
I-3108
Lawrenceville, GA 30043 USA - Phone: 404-450-8111
- Email: eharrison1@ggc.edu
- Name: Dr. Joel Trexler
- Address: 3000 NE 151 St
North Miami, FL 33181 USA - Phone: 305-348-1966
- Fax: 305-919-4030
- Email: trexlerj@fiu.edu
- URL: http://faculty.fiu.edu/~trexlerj/research_interests.htm
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Dataset AbstractCytochrome b sequences obtained from samples of Cichlasoma urophthalmus collected within the Florida Everglades and Central America. Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida.
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Geographic CoverageStudy Extent Description
ENP, west coast of south Florida, Mexico, Belize, Honduras, Guatemala, and Nicaragua
Bounding Coordinates
Data were collected within ENP, in urban Miami canals and in Mexico, Belize, Guatemala, Honduras, and Nicaragua
N: 26.634, S: 26.203, E: -81.771, W: -81.850
Florida Coastal Everglades LTER Study Area: South Florida, Everglades National Park, and Florida Bay
N: 25.761, S: 24.913, E: -80.490, W: -81.078
Mexico
N: 24.692, S: 18.709, E: -88.197, W: -91.583
Belize
N: 17.625, S: 17.021, E: -88.192, W: -88.494
Guatemala
N: 16.973, S: 16.929, E: -89.640, W: -89.891
Honduras
N: 15.848, S: 15.092, E: -85.912, W: -88.216
Nicaragua
N: 14.187, S: 13.414, E: -83.305, W: -83.571
Geographic DescriptionBounding CoordinatesLoopRd2N: 25.76, S: 25.76, E: -80.77, W: -80.77L31W2N: 25.45, S: 25.45, E: -80.56, W: -80.56TaftPalm2N: 26.022340, S: 26.022340, E: -80.28, W: -80.28216canal2N: 25.77, S: 25.77, E: -80.31, W: -80.31Aerojet2N: 25.37, S: 25.37, E: -80.54, W: -80.54MiamiSprings2N: 25.822320, S: 25.822320, E: -80.29, W: -80.29WhiskeyCr2N: 26.58, S: 26.58, E: -81.88, W: -81.88NaplesBay2N: 26.17, S: 26.17, E: -81.79, W: -81.79SpringCr2N: 26.39, S: 26.39, E: -81.85, W: -81.85ChetumalRiv2N: 17.51, S: 17.51, E: -88.22, W: -88.22CrookedTree2N: 17.77, S: 17.77, E: -88.53, W: -88.53Dangriga2N: 16.97, S: 16.97, E: -88.23, W: -88.23Airport LakesN: 25.79, S: 25.79, E: -80.27, W: -80.27La Bocana2N: 21.19, S: 21.19, E: -89.95, W: -89.95LagDeGua2N: 16.00, S: 16.00, E: -85.90, W: -85.9057th AvenueN: 25.74, S: 25.74, E: -80.29, W: -80.29NewRiver2N: 17.56, S: 17.56, E: -88.53, W: -88.53Water Conservation Area 3AN: 25.76, S: 25.76, E: -80.69, W: -80.69Everglades Gun Range canalN: 25.76, S: 25.76, E: -80.48, W: -80.48Krome AvenueN: 25.90, S: 25.90, E: -80.45, W: -80.45Omoa2N: 15.77, S: 15.77, E: -87.00, W: -87.00Marker 50N: 26.15, S: 26.15, E: -81.35, W: -81.35RiaCel2N: 20.75, S: 20.75, E: -90.35, W: -90.35RiaLag2N: 21.60, S: 21.60, E: -88.16, W: -88.16StJohnsCollege2N: 17.51, S: 17.51, E: -88.20, W: -88.20VernonSt2N: 17.50, S: 17.50, E: -88.20, W: -88.20441 WN: 26.27, S: 26.27, E: -80.20, W: -80.20C-111 CanalN: 25.34, S: 25.34, E: -80.58, W: -80.58CorkscrewN: 26.44, S: 26.44, E: -81.81, W: -81.81Punta Gorda DitchN: 26.96, S: 26.96, E: -81.95, W: -81.95Montego Bay CondominiumsN: 26.69, S: 26.69, E: -81.73, W: -81.73Henry CreekN: 26.52, S: 26.52, E: -81.88, W: -81.88Coral WatersN: 26.57, S: 26.57, E: -81.84, W: -81.84Laguna de Terminós*N: 18.71, S: 18.71, E: -91.63, W: -91.63Ría PalizadaN: 18.25, S: 18.25, E: -92.10, W: -92.10Río ChampotónN: 19.28, S: 19.28, E: -90.62, W: -90.62Río Candelaria at ZaragozaN: 18.39, S: 18.39, E: -91.31, W: -91.31Laguna de AtastaN: 18.62, S: 18.62, E: -92.10, W: -92.10Peninsula el PalmarN: 18.93, S: 18.93, E: -91.33, W: -91.33SilvitucN: 18.62, S: 18.62, E: -90.29, W: -90.29TuxtepecN: 18.08, S: 18.08, E: -96.12, W: -96.12Sian Ka'anN: 19.20, S: 19.20, E: -87.89, W: -87.89Benito JuarezN: 18.81, S: 18.81, E: -88.29, W: -88.29Laguna Noh-BekN: 19.14, S: 19.14, E: -88.17, W: -88.17Río Escondido at UcumN: 18.50, S: 18.50, E: -88.53, W: -88.53Laguna GuerreroN: 18.70, S: 18.70, E: -88.25, W: -88.25ChilapaN: 18.20, S: 18.20, E: -92.67, W: -92.67EpinoN: 18.25, S: 18.25, E: -92.84, W: -92.84Laguna Santa AnitaN: 18.38, S: 18.38, E: -92.90, W: -92.90Pantanos de CentlaN: 18.47, S: 18.47, E: -92.65, W: -92.65Laguna El RosarioN: 17.90, S: 17.90, E: -93.89, W: -93.89Laguna CanitzanN: 17.58, S: 17.58, E: -91.39, W: -91.39VillahermosaN: 18.05, S: 18.05, E: -92.93, W: -92.93Ría Celestun*N: 20.75, S: 20.75, E: -90.35, W: -90.35Zoh LagunaN: 20.84, S: 20.84, E: -86.90, W: -86.90XlacahN: 21.08, S: 21.08, E: -89.60, W: -89.60Cenote Ya-Bal-Ha*N: 21.59, S: 21.59, E: -88.08, W: -88.08Ría Lagartos*N: 21.60, S: 21.60, E: -88.16, W: -88.16Cenote Zaci*N: 20.69, S: 20.69, E: -88.20, W: -88.20Cenote Xtoloc*N: 20.68, S: 20.68, E: -88.57, W: -88.57Cenote Chen-háN: 20.69, S: 20.69, E: -89.88, W: -89.88Estero ProgresoN: 21.28, S: 21.28, E: -89.67, W: -89.67Cenote Ya´ax-ek´N: 20.62, S: 20.62, E: -88.40, W: -88.40La Bocana*N: 21.19, S: 21.19, E: -89.95, W: -89.95Río PapaloapanN: 18.61, S: 18.61, E: -95.66, W: -95.66Maskal RiverN: 17.88, S: 17.88, E: -88.31, W: -88.31Copper BankN: 18.33, S: 18.33, E: -88.35, W: -88.35Sibun RiverN: 17.46, S: 17.46, E: -88.39, W: -88.39Orange WalkN: 17.76, S: 17.76, E: -88.86, W: -88.86New River*N: 17.56, S: 17.56, E: -88.53, W: -88.53Sarstoon RiverN: 15.96, S: 15.96, E: -89.00, W: -89.00Omoa*N: 15.77, S: 15.77, E: -87.00, W: -87.00Laguna de Guaymoreto*N: 16.00, S: 16.00, E: -85.90, W: -85.90Lago Petén-Itza*N: 16.99, S: 16.99, E: -89.82, W: -89.82Laguna Macanche*N: 16.97, S: 16.97, E: -89.63, W: -89.63Laguna de Wouhnta*N: 13.60, S: 13.60, E: -83.53, W: -83.53Puerto Cabezas*N: 14.05, S: 14.05, E: -83.40, W: -83.40Laguna de Karata*N: 13.93, S: 13.93, E: -83.50, W: -83.50
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Temporal CoverageStart Date: 2012-01-10
End Date: 2014-05-30
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Attributes
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Data Table: Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central AmericaAttribute Name:SampleIDAttribute Label:Sample IDAttribute Definition:Sample IDStorage Type:textMeasurement Scale:Sample IDMissing Value Code:Attribute Name:SequenceAttribute Label:Cytochrome b base sequenceAttribute Definition:Cytochrome b base sequenceStorage Type:textMeasurement Scale:Cytochrome b base sequenceMissing Value Code:
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Data Table: Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America
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MethodsSampling Description
Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes software
Method Step
Description
Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes software
Citation
Harrison, Elizabeth 2014-09-03. Genetic evidence for multiple sources of the non-native fish Cichlasoma urophthalmus (Günther; Mayan Cichlids) in southern Florida. Plos One, 9(9): doi:10.1371/journal.pone.0104173.
ProtocolProtocol Title: Cytochrome b sequences
Protocol Creator(s)- Name: Dr. Elizabeth Harrison
Publication Date: 2013-02-27
Abstract
We isolated and sequenced the cytochrome b gene from the Mayan cichlid, Cichlasoma urophthalmus.
Keywords
Cytochrome b sequences
Procedural Steps
See article above
Instrumentation
Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes softwareQuality Control
Data was checked and double-checked for errors
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Distribution and Intellectual RightsOnline distribution
https://fce-lter.fiu.edu/perl/public_data_download.pl?datasetid=ST_TDCS_Harrison_002.txt
Data Submission Date: 2014-11-04
Intellectual Rights
This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.
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Keywordsconsumers, fishes, urban, FCE, FCE LTER, Florida Coastal Everglades LTER, ecological research, long-term monitoring, Cichlasoma urophthalmus microsatellite fragment size, microsatellites, Central America, DNA, South Florida, Miami, canals, cytochrome b, Cichlasoma urophthalmus
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Dataset Contact
- Name: Elizabeth Harrison
- Organization: Georgia Gwinnet College
- Address: 1000 University Center Lane
I-3108
Lawrenceville, GA 30043 USA - Phone: 404-450-8111
- Email: eharrison1@ggc.edu
- Position: Information Manager
- Organization: Florida Coastal Everglades LTER Program
- Address: Florida International University
University Park
OE 148
Miami, FL 33199 USA - Phone: 305-348-6054
- Fax: 305-348-4096
- Email: fcelter@fiu.edu
- URL: http://fcelter.fiu.edu
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Data Table and FormatData Table: Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central AmericaEntity Name:ST_TDCS_Harrison_002.txtEntity Description:Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central AmericaObject Name:ST_TDCS_Harrison_002.txtNumber of Header Lines:1Attribute Orientation:columnField Delimiter:,Number of Records:674
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Metadata Provider
- Organization: Florida Coastal Everglades LTER Program
- Address: Florida International University
University Park
OE 148
Miami, FL 33199 USA - Phone: 305-348-6054
- Email: fcelter@fiu.edu
- URL: http://fcelter.fiu.edu