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Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America from January 2012 to May 2014


At a Glance


Authors: Elizabeth Harrison, Joel Trexler
Time period: 2012-01-10 to 2014-05-30
Package id: knb-lter-fce.1201.3
Dataset id: ST_TDCS_Harrison_002

How to cite:
Harrison, E., J. Trexler. 2014. Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America from January 2012 to May 2014. Environmental Data Initiative. https://doi.org/10.6073/pasta/4a07f10ec6a08e78279a506423f22305. Dataset accessed 2020-10-26.

Geographic Coverage


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Detailed Metadata


  • Dataset Abstract
    Cytochrome b sequences obtained from samples of Cichlasoma urophthalmus collected within the Florida Everglades and Central America. Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida.
  • Geographic Coverage
    Study Extent Description
    ENP, west coast of south Florida, Mexico, Belize, Honduras, Guatemala, and Nicaragua

    Bounding Coordinates
    Data were collected within ENP, in urban Miami canals and in Mexico, Belize, Guatemala, Honduras, and Nicaragua
    N: 26.634, S: 26.203, E: -81.771, W: -81.850

    Florida Coastal Everglades LTER Study Area: South Florida, Everglades National Park, and Florida Bay
    N: 25.761, S: 24.913, E: -80.490, W: -81.078

    Mexico
    N: 24.692, S: 18.709, E: -88.197, W: -91.583

    Belize
    N: 17.625, S: 17.021, E: -88.192, W: -88.494

    Guatemala
    N: 16.973, S: 16.929, E: -89.640, W: -89.891

    Honduras
    N: 15.848, S: 15.092, E: -85.912, W: -88.216

    Nicaragua
    N: 14.187, S: 13.414, E: -83.305, W: -83.571

    All Sites
    Geographic Description
    Bounding Coordinates
    LoopRd2
    N: 25.76, S: 25.76, E: -80.77, W: -80.77
    L31W2
    N: 25.45, S: 25.45, E: -80.56, W: -80.56
    TaftPalm2
    N: 26.022340, S: 26.022340, E: -80.28, W: -80.28
    216canal2
    N: 25.77, S: 25.77, E: -80.31, W: -80.31
    Aerojet2
    N: 25.37, S: 25.37, E: -80.54, W: -80.54
    MiamiSprings2
    N: 25.822320, S: 25.822320, E: -80.29, W: -80.29
    WhiskeyCr2
    N: 26.58, S: 26.58, E: -81.88, W: -81.88
    NaplesBay2
    N: 26.17, S: 26.17, E: -81.79, W: -81.79
    SpringCr2
    N: 26.39, S: 26.39, E: -81.85, W: -81.85
    ChetumalRiv2
    N: 17.51, S: 17.51, E: -88.22, W: -88.22
    CrookedTree2
    N: 17.77, S: 17.77, E: -88.53, W: -88.53
    Dangriga2
    N: 16.97, S: 16.97, E: -88.23, W: -88.23
    Airport Lakes
    N: 25.79, S: 25.79, E: -80.27, W: -80.27
    La Bocana2
    N: 21.19, S: 21.19, E: -89.95, W: -89.95
    LagDeGua2
    N: 16.00, S: 16.00, E: -85.90, W: -85.90
    57th Avenue
    N: 25.74, S: 25.74, E: -80.29, W: -80.29
    NewRiver2
    N: 17.56, S: 17.56, E: -88.53, W: -88.53
    Water Conservation Area 3A
    N: 25.76, S: 25.76, E: -80.69, W: -80.69
    Everglades Gun Range canal
    N: 25.76, S: 25.76, E: -80.48, W: -80.48
    Krome Avenue
    N: 25.90, S: 25.90, E: -80.45, W: -80.45
    Omoa2
    N: 15.77, S: 15.77, E: -87.00, W: -87.00
    Marker 50
    N: 26.15, S: 26.15, E: -81.35, W: -81.35
    RiaCel2
    N: 20.75, S: 20.75, E: -90.35, W: -90.35
    RiaLag2
    N: 21.60, S: 21.60, E: -88.16, W: -88.16
    StJohnsCollege2
    N: 17.51, S: 17.51, E: -88.20, W: -88.20
    VernonSt2
    N: 17.50, S: 17.50, E: -88.20, W: -88.20
    441 W
    N: 26.27, S: 26.27, E: -80.20, W: -80.20
    C-111 Canal
    N: 25.34, S: 25.34, E: -80.58, W: -80.58
    Corkscrew
    N: 26.44, S: 26.44, E: -81.81, W: -81.81
    Punta Gorda Ditch
    N: 26.96, S: 26.96, E: -81.95, W: -81.95
    Montego Bay Condominiums
    N: 26.69, S: 26.69, E: -81.73, W: -81.73
    Henry Creek
    N: 26.52, S: 26.52, E: -81.88, W: -81.88
    Coral Waters
    N: 26.57, S: 26.57, E: -81.84, W: -81.84
    Laguna de Terminós*
    N: 18.71, S: 18.71, E: -91.63, W: -91.63
    Ría Palizada
    N: 18.25, S: 18.25, E: -92.10, W: -92.10
    Río Champotón
    N: 19.28, S: 19.28, E: -90.62, W: -90.62
    Río Candelaria at Zaragoza
    N: 18.39, S: 18.39, E: -91.31, W: -91.31
    Laguna de Atasta
    N: 18.62, S: 18.62, E: -92.10, W: -92.10
    Peninsula el Palmar
    N: 18.93, S: 18.93, E: -91.33, W: -91.33
    Silvituc
    N: 18.62, S: 18.62, E: -90.29, W: -90.29
    Tuxtepec
    N: 18.08, S: 18.08, E: -96.12, W: -96.12
    Sian Ka'an
    N: 19.20, S: 19.20, E: -87.89, W: -87.89
    Benito Juarez
    N: 18.81, S: 18.81, E: -88.29, W: -88.29
    Laguna Noh-Bek
    N: 19.14, S: 19.14, E: -88.17, W: -88.17
    Río Escondido at Ucum
    N: 18.50, S: 18.50, E: -88.53, W: -88.53
    Laguna Guerrero
    N: 18.70, S: 18.70, E: -88.25, W: -88.25
    Chilapa
    N: 18.20, S: 18.20, E: -92.67, W: -92.67
    Epino
    N: 18.25, S: 18.25, E: -92.84, W: -92.84
    Laguna Santa Anita
    N: 18.38, S: 18.38, E: -92.90, W: -92.90
    Pantanos de Centla
    N: 18.47, S: 18.47, E: -92.65, W: -92.65
    Laguna El Rosario
    N: 17.90, S: 17.90, E: -93.89, W: -93.89
    Laguna Canitzan
    N: 17.58, S: 17.58, E: -91.39, W: -91.39
    Villahermosa
    N: 18.05, S: 18.05, E: -92.93, W: -92.93
    Ría Celestun*
    N: 20.75, S: 20.75, E: -90.35, W: -90.35
    Zoh Laguna
    N: 20.84, S: 20.84, E: -86.90, W: -86.90
    Xlacah
    N: 21.08, S: 21.08, E: -89.60, W: -89.60
    Cenote Ya-Bal-Ha*
    N: 21.59, S: 21.59, E: -88.08, W: -88.08
    Ría Lagartos*
    N: 21.60, S: 21.60, E: -88.16, W: -88.16
    Cenote Zaci*
    N: 20.69, S: 20.69, E: -88.20, W: -88.20
    Cenote Xtoloc*
    N: 20.68, S: 20.68, E: -88.57, W: -88.57
    Cenote Chen-há
    N: 20.69, S: 20.69, E: -89.88, W: -89.88
    Estero Progreso
    N: 21.28, S: 21.28, E: -89.67, W: -89.67
    Cenote Ya´ax-ek´
    N: 20.62, S: 20.62, E: -88.40, W: -88.40
    La Bocana*
    N: 21.19, S: 21.19, E: -89.95, W: -89.95
    Río Papaloapan
    N: 18.61, S: 18.61, E: -95.66, W: -95.66
    Maskal River
    N: 17.88, S: 17.88, E: -88.31, W: -88.31
    Copper Bank
    N: 18.33, S: 18.33, E: -88.35, W: -88.35
    Sibun River
    N: 17.46, S: 17.46, E: -88.39, W: -88.39
    Orange Walk
    N: 17.76, S: 17.76, E: -88.86, W: -88.86
    New River*
    N: 17.56, S: 17.56, E: -88.53, W: -88.53
    Sarstoon River
    N: 15.96, S: 15.96, E: -89.00, W: -89.00
    Omoa*
    N: 15.77, S: 15.77, E: -87.00, W: -87.00
    Laguna de Guaymoreto*
    N: 16.00, S: 16.00, E: -85.90, W: -85.90
    Lago Petén-Itza*
    N: 16.99, S: 16.99, E: -89.82, W: -89.82
    Laguna Macanche*
    N: 16.97, S: 16.97, E: -89.63, W: -89.63
    Laguna de Wouhnta*
    N: 13.60, S: 13.60, E: -83.53, W: -83.53
    Puerto Cabezas*
    N: 14.05, S: 14.05, E: -83.40, W: -83.40
    Laguna de Karata*
    N: 13.93, S: 13.93, E: -83.50, W: -83.50
  • Methods
    Sampling Description
    Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes software

    Method Step

    Description
    Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes software

    Citation
    Harrison, Elizabeth 2014-09-03. Genetic evidence for multiple sources of the non-native fish Cichlasoma urophthalmus (Günther; Mayan Cichlids) in southern Florida. Plos One, 9(9): doi:10.1371/journal.pone.0104173.

    Protocol

    Protocol Title:  Cytochrome b sequences

    Protocol Creator(s)
    • Name: Dr.  Elizabeth Harrison 


    Publication Date:  2013-02-27

    Abstract
    We isolated and sequenced the cytochrome b gene from the Mayan cichlid, Cichlasoma urophthalmus.

    Keywords
    Cytochrome b sequences
    Procedural Steps
    See article above

    Instrumentation
    Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes software

    Quality Control
    Data was checked and double-checked for errors
  • Distribution and Intellectual Rights
    Online distribution
    https://fce-lter.fiu.edu/perl/public_data_download.pl?datasetid=ST_TDCS_Harrison_002.txt
    Data Submission Date:  2014-11-04

    Intellectual Rights
    This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

  • Keywords
    consumers, fishes, urban, FCE, FCE LTER, Florida Coastal Everglades LTER, ecological research, long-term monitoring, Cichlasoma urophthalmus microsatellite fragment size, microsatellites, Central America, DNA, South Florida, Miami, canals, cytochrome b, Cichlasoma urophthalmus
  • Dataset Contact
    • Name: Elizabeth Harrison 
    • Organization: Georgia Gwinnet College
    • Address: 1000 University Center Lane
      I-3108
      Lawrenceville, GA 30043 USA
    • Phone: 404-450-8111
    • Email: eharrison1@ggc.edu

    • Position: Information Manager
    • Organization: Florida Coastal Everglades LTER Program
    • Address: Florida International University
      University Park
      OE 148
      Miami, FL 33199 USA
    • Phone: 305-348-6054
    • Fax: 305-348-4096
    • Email: fcelter@fiu.edu
    • URL: http://fcelter.fiu.edu

  • Data Table and Format
    Data Table:  Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America

    Entity Name:
    ST_TDCS_Harrison_002.txt
    Entity Description:
    Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America
    Object Name:
    ST_TDCS_Harrison_002.txt
    Number of Header Lines:
    1
    Attribute Orientation:
    column
    Field Delimiter:
    ,
    Number of Records:
    674