Florida Coastal Everglades LTER

Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America from January 2012 to May 2014

At a Glance

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Authors: Elizabeth Harrison, Joel Trexler
Time period: to
Package id: knb-lter-fce.1201.2
Dataset id: ST_TDCS_Harrison_002

How to cite:
Harrison, E., J. Trexler. 2014. Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America from January 2012 to May 2014. Environmental Data Initiative. https://doi.org/. Dataset accessed 2024-04-16.

Geographic Coverage

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Detailed Metadata

Dataset Creator(s)
- Name: Dr. Elizabeth Harrison
- Position: Assistant Professor
- Organization: Florida Coastal Everglades LTER Program
- Address: Georgia Gwinnet College
  1000 University Center Lane
  I-3108
  Lawrenceville, GA 30043 USA
- Phone: 404-450-8111
- Email: eharrison1@ggc.edu
- Name: Dr. Joel Trexler
- Position: Director of Marine Science
- Organization: Florida Coastal Everglades LTER Program
- Address: 3000 NE 151 St
  North Miami, FL 33181 USA
- Phone: 305-348-1966
- Fax: 305-919-4030
- Email: trexlerj@fiu.edu
- URL: http://faculty.fiu.edu/~trexlerj/research_interests.htm

Dataset Abstract
Cytochrome b sequences obtained from samples of Cichlasoma urophthalmus collected within the Florida Everglades and Central America. Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida.

Geographic Coverage

Study Extent Description
ENP, west coast of south Florida, Mexico, Belize, Honduras, Guatemala, and Nicaragua

Bounding Coordinates
Data were collected within ENP, in urban Miami canals and in Mexico, Belize, Guatemala, Honduras, and Nicaragua
N: 26.634, S: 26.203, E: -81.771, W: -81.850

Florida Coastal Everglades LTER Study Area: South Florida, Everglades National Park, and Florida Bay
N: 25.761, S: 24.913, E: -80.490, W: -81.078

Mexico
N: 18.709, S: 24.692, E: -88.197, W: -91.583

Belize
N: 17.625, S: 17.021, E: -88.192, W: -88.494

Guatemala
N: 16.929, S: 16.973, E: -89.640, W: -89.891

Honduras
N: 15.848, S: 15.092, E: -85.912, W: -88.216

Nicaragua
N: 13.414, S: 14.187, E: -83.305, W: -83.571

All Sites

Geographic Description

Bounding Coordinates

LoopRd2

N: 25.76, S: 25.76, E: -80.77, W: -80.77

L31W2

N: 25.45, S: 25.45, E: -80.56, W: -80.56

TaftPalm2

N: 26.022340, S: 26.022340, E: -80.28, W: -80.28

216canal2

N: 25.77, S: 25.77, E: -80.31, W: -80.31

Aerojet2

N: 25.37, S: 25.37, E: -80.54, W: -80.54

MiamiSprings2

N: 25.822320, S: 25.822320, E: -80.29, W: -80.29

WhiskeyCr2

N: 26.58, S: 26.58, E: -81.88, W: -81.88

NaplesBay2

N: 26.17, S: 26.17, E: -81.79, W: -81.79

SpringCr2

N: 26.39, S: 26.39, E: -81.85, W: -81.85

ChetumalRiv2

N: 17.51, S: 17.51, E: -88.22, W: -88.22

CrookedTree2

N: 17.77, S: 17.77, E: -88.53, W: -88.53

Dangriga2

N: 16.97, S: 16.97, E: -88.23, W: -88.23

Airport Lakes

N: 25.79, S: 25.79, E: -80.27, W: -80.27

La Bocana2

N: 21.19, S: 21.19, E: -89.95, W: -89.95

LagDeGua2

N: 16.00, S: 16.00, E: -85.90, W: -85.90

57th Avenue

N: 25.74, S: 25.74, E: -80.29, W: -80.29

NewRiver2

N: 17.56, S: 17.56, E: -88.53, W: -88.53

Water Conservation Area 3A

N: 25.76, S: 25.76, E: -80.69, W: -80.69

Everglades Gun Range canal

N: 25.76, S: 25.76, E: -80.48, W: -80.48

Krome Avenue

N: 25.90, S: 25.90, E: -80.45, W: -80.45

Omoa2

N: 15.77, S: 15.77, E: -87.00, W: -87.00

Marker 50

N: 26.15, S: 26.15, E: -81.35, W: -81.35

RiaCel2

N: 20.75, S: 20.75, E: -90.35, W: -90.35

RiaLag2

N: 21.60, S: 21.60, E: -88.16, W: -88.16

StJohnsCollege2

N: 17.51, S: 17.51, E: -88.20, W: -88.20

VernonSt2

N: 17.50, S: 17.50, E: -88.20, W: -88.20

441 W

N: 26.27, S: 26.27, E: -80.20, W: -80.20

C-111 Canal

N: 25.34, S: 25.34, E: -80.58, W: -80.58

Corkscrew

N: 26.44, S: 26.44, E: -81.81, W: -81.81

Punta Gorda Ditch

N: 26.96, S: 26.96, E: -81.95, W: -81.95

Montego Bay Condominiums

N: 26.69, S: 26.69, E: -81.73, W: -81.73

Henry Creek

N: 26.52, S: 26.52, E: -81.88, W: -81.88

Coral Waters

N: 26.57, S: 26.57, E: -81.84, W: -81.84

Laguna de Terminós*

N: 18.71, S: 18.71, E: -91.63, W: -91.63

Ria Palizada

N: 18.25, S: 18.25, E: -92.10, W: -92.10

Rio Champotón

N: 19.28, S: 19.28, E: -90.62, W: -90.62

Rio Candelaria at Zaragoza

N: 18.39, S: 18.39, E: -91.31, W: -91.31

Laguna de Atasta

N: 18.62, S: 18.62, E: -92.10, W: -92.10

Peninsula el Palmar

N: 18.93, S: 18.93, E: -91.33, W: -91.33

Silvituc

N: 18.62, S: 18.62, E: -90.29, W: -90.29

Tuxtepec

N: 18.08, S: 18.08, E: -96.12, W: -96.12

Sian Kaan

N: 19.20, S: 19.20, E: -87.89, W: -87.89

Benito Juarez

N: 18.81, S: 18.81, E: -88.29, W: -88.29

Laguna Noh-Bek

N: 19.14, S: 19.14, E: -88.17, W: -88.17

Rio Escondido at Ucum

N: 18.50, S: 18.50, E: -88.53, W: -88.53

Laguna Guerrero

N: 18.70, S: 18.70, E: -88.25, W: -88.25

Chilapa

N: 18.20, S: 18.20, E: -92.67, W: -92.67

Epino

N: 18.25, S: 18.25, E: -92.84, W: -92.84

Laguna Santa Anita

N: 18.38, S: 18.38, E: -92.90, W: -92.90

Pantanos de Centla

N: 18.47, S: 18.47, E: -92.65, W: -92.65

Laguna El Rosario

N: 17.90, S: 17.90, E: -93.89, W: -93.89

Laguna Canitzan

N: 17.58, S: 17.58, E: -91.39, W: -91.39

Villahermosa

N: 18.05, S: 18.05, E: -92.93, W: -92.93

Ria Celestun

N: 20.75, S: 20.75, E: -90.35, W: -90.35

Zoh Laguna

N: 20.84, S: 20.84, E: -86.90, W: -86.90

Xlacah

N: 21.08, S: 21.08, E: -89.60, W: -89.60

Cenote Ya-Bal-Ha

N: 21.59, S: 21.59, E: -88.08, W: -88.08

Ria Lagartos

N: 21.60, S: 21.60, E: -88.16, W: -88.16

Cenote Zaci

N: 20.69, S: 20.69, E: -88.20, W: -88.20

Cenote Xtoloc

N: 20.68, S: 20.68, E: -88.57, W: -88.57

Cenote Chen-ha

N: 20.69, S: 20.69, E: -89.88, W: -89.88

Estero Progreso

N: 21.28, S: 21.28, E: -89.67, W: -89.67

Cenote Ya ax-ek

N: 20.62, S: 20.62, E: -88.40, W: -88.40

La Bocana

N: 21.19, S: 21.19, E: -89.95, W: -89.95

Rio Papaloapan

N: 18.61, S: 18.61, E: -95.66, W: -95.66

Maskal River

N: 17.88, S: 17.88, E: -88.31, W: -88.31

Copper Bank

N: 18.33, S: 18.33, E: -88.35, W: -88.35

Sibun River

N: 17.46, S: 17.46, E: -88.39, W: -88.39

Orange Walk

N: 17.76, S: 17.76, E: -88.86, W: -88.86

New River

N: 17.56, S: 17.56, E: -88.53, W: -88.53

Sarstoon River

N: 15.96, S: 15.96, E: -89.00, W: -89.00

Omoa

N: 15.77, S: 15.77, E: -87.00, W: -87.00

Laguna de Guaymoreto

N: 16.00, S: 16.00, E: -85.90, W: -85.90

Lago Peten-Itza

N: 16.99, S: 16.99, E: -89.82, W: -89.82

Laguna Macanche

N: 16.97, S: 16.97, E: -89.63, W: -89.63

Laguna de Wouhnta

N: 13.60, S: 13.60, E: -83.53, W: -83.53

Puerto Cabezas

N: 14.05, S: 14.05, E: -83.40, W: -83.40

Laguna de Karata

N: 13.93, S: 13.93, E: -83.50, W: -83.50

Temporal Coverage
Start Date: 2012-01-10
End Date: 2014-05-30

Attributes
- Data Table: Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America
  
  Attribute Name:
  
  SampleID
  
  Attribute Label:
  
  Sample ID
  
  Attribute Definition:
  
  Sample ID
  
  Storage Type:
  
  text
  
  Measurement Scale:
  
  Sample ID
  
  Missing Value Code:
  
  Attribute Name:
  
  Sequence
  
  Attribute Label:
  
  Cytochrome b base sequence
  
  Attribute Definition:
  
  Cytochrome b base sequence
  
  Storage Type:
  
  text
  
  Measurement Scale:
  
  Cytochrome b base sequence
  
  Missing Value Code:

Methods
Sampling Description
Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes software
Method Step
Description
Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes software

Citation
Harrison, Elizabeth 2014-09-03. Genetic evidence for multiple sources of the non-native fish Cichlasoma urophthalmus (Günther; Mayan Cichlids) in southern Florida. Plos One, 9(9): doi:10.1371/journal.pone.0104173.

Protocol
Protocol Title: Cytochrome b sequences

Protocol Creator(s)
Name: Dr. Elizabeth Harrison

Publication Date: 2013-02-27

Abstract
We isolated and sequenced the cytochrome b gene from the Mayan cichlid, Cichlasoma urophthalmus.

Keywords
Cytochrome b sequences
Procedural Steps
See article above
Instrumentation
Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes software
Quality Control
Data was checked and double-checked for errors

Distribution and Intellectual Rights
Online distribution
http://fcelter.fiu.edu/perl/public_data_download.pl?datasetid=ST_TDCS_Harrison_002.txt
Data Submission Date: 2014-11-04

Intellectual Rights
These data are classified as 'Type I' whereby original FCE LTER experimental data collected by individual FCE researchers are available for scientific purposes 2 years after collection. Maximum lock period = 2 years. All publications based on this dataset must cite the data Contributor, the Florida Coastal Everglades Long-Term Ecological Research (LTER) Program and that this material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DBI-0620409, and #DEB-9910514. Additionally, two copies of the manuscript must be submitted to the Florida Coastal Everglades LTER Program Office, LTER Program Manager, Florida International University, Southeast Environmental Research Center, OE 148, University Park, Miami, Florida 33199. For a complete description of the FCE LTER Data Distribution and Data User Agreement, please go to FCE Data Management Policy at http://fcelter.fiu.edu/data/DataMgmt.pdf and LTER Network Data Access Policy at http://fcelter.fiu.edu/data/core/data_user_agreement/distribution_policy.html.

Keywords
FCE, Florida Coastal Everglades LTER, ecological research, long-term monitoring, Cichlasoma urophthalmus microsatellite fragment size, microsatellites, Central America, DNA, South Florida, urban Miami, Miami, canals, consumers, fish, cytochrome b, Cichlasoma urophthalmus

Dataset Contact
- Position: Information Manager
- Organization: LTER Network Office
- Address: UNM Biology Department, MSC03-2020
  1 University of New Mexico
  Albuquerque, NM 87131-0001 USA
- Phone: 505 277-2535
- Fax: 505 277-2541
- Email: tech-support@lternet.edu
- URL: http://www.lternet.edu
- Name: Elizabeth Harrison
- Organization: Georgia Gwinnet College
- Address: 1000 University Center Lane
  I-3108
  Lawrenceville, GA 30043 USA
- Phone: 404-450-8111
- Email: eharrison1@ggc.edu
- Position: Information Manager
- Organization: Florida Coastal Everglades LTER Program
- Address: Florida International University
  University Park
  OE 148
  Miami, FL 33199 USA
- Phone: 305-348-6054
- Fax: 305-348-4096
- Email: fcelter@fiu.edu
- URL: http://fcelter.fiu.edu

Data Table and Format
Data Table: Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America

Entity Name:

ST_TDCS_Harrison_002

Entity Description:

Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America

Object Name:

ST_TDCS_Harrison_002

Number of Records:

680

Metadata Provider
- Organization: Florida Coastal Everglades LTER Program
- Address: Florida International University
  University Park
  OE 148
  Miami, FL 33199 USA
- Phone: 305-348-6054
- Email: fcelter@fiu.edu
- URL: http://fcelter.fiu.edu

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Metadata

Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America from January 2012 to May 2014

At a Glance

Detailed Metadata