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Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America from January 2012 to May 2014


At a Glance


Authors: Elizabeth Harrison, Joel Trexler
Time period: 2012-01-10 to 2014-05-30
Package id: knb-lter-fce.1201.2
Dataset id: ST_TDCS_Harrison_002

How to cite:
Harrison, E., J. Trexler. 2014. Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America from January 2012 to May 2014. Environmental Data Initiative. https://doi.org/10.6073/pasta/406058160f1adb10a2ec578c56db5df8. Dataset accessed 2020-02-28.

Geographic Coverage


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Detailed Metadata


  • Dataset Creator(s)
    • Name: Dr.  Elizabeth Harrison 
    • Position: Assistant Professor
    • Organization: Florida Coastal Everglades LTER Program
    • Address: Georgia Gwinnet College
      1000 University Center Lane
      I-3108
      Lawrenceville, GA 30043 USA
    • Phone: 404-450-8111
    • Email: eharrison1@ggc.edu


  • Dataset Abstract
    Cytochrome b sequences obtained from samples of Cichlasoma urophthalmus collected within the Florida Everglades and Central America. Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida.
  • Geographic Coverage
    Study Extent Description
    ENP, west coast of south Florida, Mexico, Belize, Honduras, Guatemala, and Nicaragua

    Bounding Coordinates
    Data were collected within ENP, in urban Miami canals and in Mexico, Belize, Guatemala, Honduras, and Nicaragua
    N: 26.634, S: 26.203, E: -81.771, W: -81.850

    Florida Coastal Everglades LTER Study Area: South Florida, Everglades National Park, and Florida Bay
    N: 25.761, S: 24.913, E: -80.490, W: -81.078

    Mexico
    N: 18.709, S: 24.692, E: -88.197, W: -91.583

    Belize
    N: 17.625, S: 17.021, E: -88.192, W: -88.494

    Guatemala
    N: 16.929, S: 16.973, E: -89.640, W: -89.891

    Honduras
    N: 15.848, S: 15.092, E: -85.912, W: -88.216

    Nicaragua
    N: 13.414, S: 14.187, E: -83.305, W: -83.571

    All Sites
    Geographic Description
    Bounding Coordinates
    LoopRd2
    N: 25.76, S: 25.76, E: -80.77, W: -80.77
    L31W2
    N: 25.45, S: 25.45, E: -80.56, W: -80.56
    TaftPalm2
    N: 26.022340, S: 26.022340, E: -80.28, W: -80.28
    216canal2
    N: 25.77, S: 25.77, E: -80.31, W: -80.31
    Aerojet2
    N: 25.37, S: 25.37, E: -80.54, W: -80.54
    MiamiSprings2
    N: 25.822320, S: 25.822320, E: -80.29, W: -80.29
    WhiskeyCr2
    N: 26.58, S: 26.58, E: -81.88, W: -81.88
    NaplesBay2
    N: 26.17, S: 26.17, E: -81.79, W: -81.79
    SpringCr2
    N: 26.39, S: 26.39, E: -81.85, W: -81.85
    ChetumalRiv2
    N: 17.51, S: 17.51, E: -88.22, W: -88.22
    CrookedTree2
    N: 17.77, S: 17.77, E: -88.53, W: -88.53
    Dangriga2
    N: 16.97, S: 16.97, E: -88.23, W: -88.23
    Airport Lakes
    N: 25.79, S: 25.79, E: -80.27, W: -80.27
    La Bocana2
    N: 21.19, S: 21.19, E: -89.95, W: -89.95
    LagDeGua2
    N: 16.00, S: 16.00, E: -85.90, W: -85.90
    57th Avenue
    N: 25.74, S: 25.74, E: -80.29, W: -80.29
    NewRiver2
    N: 17.56, S: 17.56, E: -88.53, W: -88.53
    Water Conservation Area 3A
    N: 25.76, S: 25.76, E: -80.69, W: -80.69
    Everglades Gun Range canal
    N: 25.76, S: 25.76, E: -80.48, W: -80.48
    Krome Avenue
    N: 25.90, S: 25.90, E: -80.45, W: -80.45
    Omoa2
    N: 15.77, S: 15.77, E: -87.00, W: -87.00
    Marker 50
    N: 26.15, S: 26.15, E: -81.35, W: -81.35
    RiaCel2
    N: 20.75, S: 20.75, E: -90.35, W: -90.35
    RiaLag2
    N: 21.60, S: 21.60, E: -88.16, W: -88.16
    StJohnsCollege2
    N: 17.51, S: 17.51, E: -88.20, W: -88.20
    VernonSt2
    N: 17.50, S: 17.50, E: -88.20, W: -88.20
    441 W
    N: 26.27, S: 26.27, E: -80.20, W: -80.20
    C-111 Canal
    N: 25.34, S: 25.34, E: -80.58, W: -80.58
    Corkscrew
    N: 26.44, S: 26.44, E: -81.81, W: -81.81
    Punta Gorda Ditch
    N: 26.96, S: 26.96, E: -81.95, W: -81.95
    Montego Bay Condominiums
    N: 26.69, S: 26.69, E: -81.73, W: -81.73
    Henry Creek
    N: 26.52, S: 26.52, E: -81.88, W: -81.88
    Coral Waters
    N: 26.57, S: 26.57, E: -81.84, W: -81.84
    Laguna de Terminós*
    N: 18.71, S: 18.71, E: -91.63, W: -91.63
    Ria Palizada
    N: 18.25, S: 18.25, E: -92.10, W: -92.10
    Rio Champotón
    N: 19.28, S: 19.28, E: -90.62, W: -90.62
    Rio Candelaria at Zaragoza
    N: 18.39, S: 18.39, E: -91.31, W: -91.31
    Laguna de Atasta
    N: 18.62, S: 18.62, E: -92.10, W: -92.10
    Peninsula el Palmar
    N: 18.93, S: 18.93, E: -91.33, W: -91.33
    Silvituc
    N: 18.62, S: 18.62, E: -90.29, W: -90.29
    Tuxtepec
    N: 18.08, S: 18.08, E: -96.12, W: -96.12
    Sian Kaan
    N: 19.20, S: 19.20, E: -87.89, W: -87.89
    Benito Juarez
    N: 18.81, S: 18.81, E: -88.29, W: -88.29
    Laguna Noh-Bek
    N: 19.14, S: 19.14, E: -88.17, W: -88.17
    Rio Escondido at Ucum
    N: 18.50, S: 18.50, E: -88.53, W: -88.53
    Laguna Guerrero
    N: 18.70, S: 18.70, E: -88.25, W: -88.25
    Chilapa
    N: 18.20, S: 18.20, E: -92.67, W: -92.67
    Epino
    N: 18.25, S: 18.25, E: -92.84, W: -92.84
    Laguna Santa Anita
    N: 18.38, S: 18.38, E: -92.90, W: -92.90
    Pantanos de Centla
    N: 18.47, S: 18.47, E: -92.65, W: -92.65
    Laguna El Rosario
    N: 17.90, S: 17.90, E: -93.89, W: -93.89
    Laguna Canitzan
    N: 17.58, S: 17.58, E: -91.39, W: -91.39
    Villahermosa
    N: 18.05, S: 18.05, E: -92.93, W: -92.93
    Ria Celestun
    N: 20.75, S: 20.75, E: -90.35, W: -90.35
    Zoh Laguna
    N: 20.84, S: 20.84, E: -86.90, W: -86.90
    Xlacah
    N: 21.08, S: 21.08, E: -89.60, W: -89.60
    Cenote Ya-Bal-Ha
    N: 21.59, S: 21.59, E: -88.08, W: -88.08
    Ria Lagartos
    N: 21.60, S: 21.60, E: -88.16, W: -88.16
    Cenote Zaci
    N: 20.69, S: 20.69, E: -88.20, W: -88.20
    Cenote Xtoloc
    N: 20.68, S: 20.68, E: -88.57, W: -88.57
    Cenote Chen-ha
    N: 20.69, S: 20.69, E: -89.88, W: -89.88
    Estero Progreso
    N: 21.28, S: 21.28, E: -89.67, W: -89.67
    Cenote Ya ax-ek
    N: 20.62, S: 20.62, E: -88.40, W: -88.40
    La Bocana
    N: 21.19, S: 21.19, E: -89.95, W: -89.95
    Rio Papaloapan
    N: 18.61, S: 18.61, E: -95.66, W: -95.66
    Maskal River
    N: 17.88, S: 17.88, E: -88.31, W: -88.31
    Copper Bank
    N: 18.33, S: 18.33, E: -88.35, W: -88.35
    Sibun River
    N: 17.46, S: 17.46, E: -88.39, W: -88.39
    Orange Walk
    N: 17.76, S: 17.76, E: -88.86, W: -88.86
    New River
    N: 17.56, S: 17.56, E: -88.53, W: -88.53
    Sarstoon River
    N: 15.96, S: 15.96, E: -89.00, W: -89.00
    Omoa
    N: 15.77, S: 15.77, E: -87.00, W: -87.00
    Laguna de Guaymoreto
    N: 16.00, S: 16.00, E: -85.90, W: -85.90
    Lago Peten-Itza
    N: 16.99, S: 16.99, E: -89.82, W: -89.82
    Laguna Macanche
    N: 16.97, S: 16.97, E: -89.63, W: -89.63
    Laguna de Wouhnta
    N: 13.60, S: 13.60, E: -83.53, W: -83.53
    Puerto Cabezas
    N: 14.05, S: 14.05, E: -83.40, W: -83.40
    Laguna de Karata
    N: 13.93, S: 13.93, E: -83.50, W: -83.50
  • Methods
    Sampling Description
    Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes software

    Method Step

    Description
    Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes software

    Citation
    Harrison, Elizabeth 2014-09-03. Genetic evidence for multiple sources of the non-native fish Cichlasoma urophthalmus (Günther; Mayan Cichlids) in southern Florida. Plos One, 9(9): doi:10.1371/journal.pone.0104173.

    Protocol

    Protocol Title:  Cytochrome b sequences

    Protocol Creator(s)
    • Name: Dr.  Elizabeth Harrison 


    Publication Date:  2013-02-27

    Abstract
    We isolated and sequenced the cytochrome b gene from the Mayan cichlid, Cichlasoma urophthalmus.

    Keywords
    Cytochrome b sequences
    Procedural Steps
    See article above

    Instrumentation
    Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and cytochrome b was sequenced. Data were used to determine the source for Mayan Cichlids in Florida using Mr. Bayes software

    Quality Control
    Data was checked and double-checked for errors
  • Distribution and Intellectual Rights
    Online distribution
    http://fcelter.fiu.edu/perl/public_data_download.pl?datasetid=ST_TDCS_Harrison_002.txt
    Data Submission Date:  2014-11-04

    Intellectual Rights
    These data are classified as 'Type I' whereby original FCE LTER experimental data collected by individual FCE researchers are available for scientific purposes 2 years after collection. Maximum lock period = 2 years. All publications based on this dataset must cite the data Contributor, the Florida Coastal Everglades Long-Term Ecological Research (LTER) Program and that this material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DBI-0620409, and #DEB-9910514. Additionally, two copies of the manuscript must be submitted to the Florida Coastal Everglades LTER Program Office, LTER Program Manager, Florida International University, Southeast Environmental Research Center, OE 148, University Park, Miami, Florida 33199. For a complete description of the FCE LTER Data Distribution and Data User Agreement, please go to FCE Data Management Policy at http://fcelter.fiu.edu/data/DataMgmt.pdf and LTER Network Data Access Policy at http://fcelter.fiu.edu/data/core/data_user_agreement/distribution_policy.html.

  • Keywords
    FCE, Florida Coastal Everglades LTER, ecological research, long-term monitoring, Cichlasoma urophthalmus microsatellite fragment size, microsatellites, Central America, DNA, South Florida, urban Miami, Miami, canals, consumers, fish, cytochrome b, Cichlasoma urophthalmus
  • Dataset Contact
    • Position: Information Manager
    • Organization: LTER Network Office
    • Address: UNM Biology Department, MSC03-2020
      1 University of New Mexico
      Albuquerque, NM 87131-0001 USA
    • Phone: 505 277-2535
    • Fax: 505 277-2541
    • Email: tech-support@lternet.edu
    • URL: http://www.lternet.edu

    • Name: Elizabeth Harrison 
    • Organization: Georgia Gwinnet College
    • Address: 1000 University Center Lane
      I-3108
      Lawrenceville, GA 30043 USA
    • Phone: 404-450-8111
    • Email: eharrison1@ggc.edu

    • Position: Information Manager
    • Organization: Florida Coastal Everglades LTER Program
    • Address: Florida International University
      University Park
      OE 148
      Miami, FL 33199 USA
    • Phone: 305-348-6054
    • Fax: 305-348-4096
    • Email: fcelter@fiu.edu
    • URL: http://fcelter.fiu.edu

  • Data Table and Format
    Data Table:  Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America

    Entity Name:
    ST_TDCS_Harrison_002
    Entity Description:
    Cichlasoma urophthalmus cytochrome b sequences collected from the Florida Everglades (FCE) and Central America
    Object Name:
    ST_TDCS_Harrison_002
    Number of Records:
    680