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Cichlasoma urophthalmus microsatellite fragment size collected from the Florida Everglades (FCE) and Central America from June 2010 to March 2013


At a Glance


Authors: Elizabeth Harrison, Joel Trexler
Time period: 2010-06-17 to 2013-03-09
Package id: knb-lter-fce.1200.4
Dataset id: ST_TDCS_Harrison_001

How to cite:
Harrison, E., J. Trexler. 2014. Cichlasoma urophthalmus microsatellite fragment size collected from the Florida Everglades (FCE) and Central America from June 2010 to March 2013. Environmental Data Initiative. https://doi.org/10.6073/pasta/de1ec3c490268a9b3d784a9266fa2ebf. Dataset accessed 2020-02-28.

Geographic Coverage


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Detailed Metadata


  • Dataset Creator(s)
    • Name: Dr.  Elizabeth Harrison 
    • Position: Assistant Professor
    • Organization: Florida Coastal Everglades LTER Program
    • Address: Georgia Gwinnet College
      1000 University Center Lane
      I-3108
      Lawrenceville, GA 30043 USA
    • Phone: 404-450-8111
    • Email: eharrison1@ggc.edu


  • Dataset Abstract
    Fragment sizes for 17 microsatellite markers obtained from samples of Cichlasoma urophthalmus collected from fin clippings of fish caught by angling and/or cast netting within the Florida Everglades and Central America. DNA was extracted, amplified and sizes of microsatellite fragments were rounded to nearest unit. Data were used to determine population genetic structure using GenAlex, Structure and DIYABC programs.
  • Geographic Coverage
    Study Extent Description
    ENP, west coast of south Florida, Mexico, Belize, Honduras, Guatemala, and Nicaragua

    Bounding Coordinates
    Data were collected within ENP, in urban Miami canals and in Mexico, Belize, Guatemala, Honduras, and Nicaragua
    N: 26.634, S: 26.203, E: -81.771, W: -81.850

    Florida Coastal Everglades LTER Study Area: South Florida, Everglades National Park, and Florida Bay
    N: 25.761, S: 24.913, E: -80.490, W: -81.078

    Mexico
    N: 18.709, S: 24.692, E: -88.197, W: -91.583

    Belize
    N: 17.625, S: 17.021, E: -88.192, W: -88.494

    Guatemala
    N: 16.929, S: 16.973, E: -89.640, W: -89.891

    Honduras
    N: 15.848, S: 15.092, E: -85.912, W: -88.216

    Nicaragua
    N: 13.414, S: 14.187, E: -83.305, W: -83.571

    All Sites
    Geographic Description
    Bounding Coordinates
    LoopRd
    N: 25.782, S: 25.782, E: -80.753, W: -80.753
    L31W
    N: 25.470, S: 25.470, E: -80.552, W: -80.552
    TaftPalm
    N: 26.043, S: 26.043, E: -80.268, W: -80.268
    216canal
    N: 25.787, S: 25.787, E: -80.033, W: -80.033
    Aerojet
    N: 25.390, S: 25.390, E: -80.527, W: -80.527
    MiamiSprings
    N: 25.835, S: 25.835, E: -80.279, W: -80.279
    WhiskeyCr
    N: 26.634, S: 26.634, E: -81.850, W: -81.850
    NaplesBay
    N: 26.203, S: 26.203, E: -81.771, W: -81.771
    SpringCr
    N: 26.423, S: 26.423, E: -81.835, W: -81.835
    ChetumalRiv
    N: 17.51, S: 17.51, E: -88.22, W: -88.22
    CrookedTree
    N: 17.852, S: 17.852, E: -88.502, W: -88.502
    Dangriga
    N: 17.020, S: 17.020, E: -88.192, W: -88.192
    Fenix
    N: 18.709, S: 18.709, E: -91.582, W: -91.582
    La Bocana
    N: 23.215, S: 23.215, E: -89.944, W: -89.944
    LagDeGua
    N: 15.092, S: 15.092, E: -85.912, W: -85.912
    Macan
    N: 16.972, S: 16.972, E: -89.640, W: -89.640
    NewRiver
    N: 17.625, S: 17.625, E: -88.494, W: -88.494
    Nica1
    N: 13.414, S: 13.414, E: -83.571, W: -83.571
    Nica3
    N: 14.187, S: 14.187, E: -83.305, W: -83.305
    Nica4
    N: 14.041, S: 14.041, E: -83.463, W: -83.463
    Omoa
    N: 15.848, S: 15.848, E: -88.216, W: -88.216
    Peten
    N: 16.929, S: 16.929, E: -89.891, W: -89.891
    RiaCel
    N: 20.904, S: 20.904, E: -90.343, W: -90.343
    RiaLag
    N: 21.598, S: 21.598, E: -88.157, W: -88.157
    StJohnsCollege
    N: 17.517, S: 17.517, E: -88.196, W: -88.196
    VernonSt
    N: 17.501, S: 17.501, E: -88.193, W: -88.193
    Xtoloc
    N: 20.680, S: 20.680, E: -88.569, W: -88.569
    YaBalHa
    N: 21.566, S: 21.566, E: -88.074, W: -88.074
    Zaci
    N: 24.692, S: 24.692, E: -88.197, W: -88.197
  • Attributes
    • Data Table:   Cichlasoma urophthalmus microsatellite fragment size collected from the Florida Everglades (FCE) and Central America
      Attribute Name:
      SampleID
      Attribute Label:
      Sample ID
      Attribute Definition:
      Sample ID
      Storage Type:
      text
      Measurement Scale:
      Sample ID
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      Date
      Attribute Definition:
      Collection Date
      Storage Type:
      datetime
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      SITENAME
      Attribute Label:
      sitename
      Attribute Definition:
      Name of Collection site
      Storage Type:
      text
      Measurement Scale:
      Name of Collection site
      Missing Value Code:
       

      Attribute Name:
      Latitude_DD
      Attribute Label:
      Latitude_DD
      Attribute Definition:
      latitude of collection location in decimal degrees
      Storage Type:
      ordinal
      Measurement Scale:
      latitude of collection location in decimal degrees
      Missing Value Code:
      -9999.000 (Value will never be recorded )

      Attribute Name:
      Longitude_DD
      Attribute Label:
      Longitude_DD
      Attribute Definition:
      longitude of collection location in decimal degrees
      Storage Type:
      ordinal
      Measurement Scale:
      longitude of collection location in decimal degrees
      Missing Value Code:
      -9999.000 (Value will never be recorded )

      Attribute Name:
      Ciur2a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur2b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur4a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur4b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur7a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur7b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur9a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur9b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur10a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur10b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur15a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur15b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur17a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur17b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur21a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur21b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur22a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur22b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur23a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur23b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur31a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur31b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur38a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur38b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur39a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur39b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur41a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur41b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur42a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur42b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur43a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur43b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur46a
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)

      Attribute Name:
      Ciur46b
      Attribute Label:
      Microsatellite marker
      Attribute Definition:
      Microsatellite marker on one chromosome of homologue. Number of bases (DNA length) of allele.
      Storage Type:
      data
      Measurement Scale:
      Units: number
      Precision: 1
      Number Type: whole
      Missing Value Code:
      -9999 (Fragment size could not be determined)


  • Methods
    Sampling Description
    Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and sizes of microsatellite fragments were rounded to nearest unit. Data were used to determine population genetic structure using GenAlex, Structure and DIYABC programs

    Method Step

    Description
    Samples were collected from fin clippings of fish caught by angling and/or cast netting. DNA was extracted, amplified and sizes of microsatellite fragments were rounded to nearest unit. Data were used to determine population genetic structure using GenAlex, Structure and DIYABC programs

    Citation
    Harrison, Elizabeth 2014-09-03. Genetic evidence for multiple sources of the non-native fish Cichlasoma urophthalmus (Günther; Mayan Cichlids) in southern Florida. Plos One, 9(9): doi:10.1371/journal.pone.0104173.

    Instrumentation
    cast nettingGenAlex Structure and DIYABC programs

    Quality Control
    Data was checked and double-checked for errors
  • Distribution and Intellectual Rights
    Online distribution
    http://fcelter.fiu.edu/perl/public_data_download.pl?datasetid=ST_TDCS_Harrison_001.txt
    Data Submission Date:  2014-11-04

    Intellectual Rights
    These data are classified as 'Type I' whereby original FCE LTER experimental data collected by individual FCE researchers are available for scientific purposes 2 years after collection. Maximum lock period = 2 years. All publications based on this dataset must cite the data Contributor, the Florida Coastal Everglades Long-Term Ecological Research (LTER) Program and that this material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DBI-0620409, and #DEB-9910514. Additionally, two copies of the manuscript must be submitted to the Florida Coastal Everglades LTER Program Office, LTER Program Manager, Florida International University, Southeast Environmental Research Center, OE 148, University Park, Miami, Florida 33199. For a complete description of the FCE LTER Data Distribution and Data User Agreement, please go to FCE Data Management Policy at http://fcelter.fiu.edu/data/DataMgmt.pdf and LTER Network Data Access Policy at http://fcelter.fiu.edu/data/core/data_user_agreement/distribution_policy.html.

  • Keywords
    FCE, Florida Coastal Everglades LTER, ecological research, long-term monitoring, Cichlasoma urophthalmus microsatellite fragment size, microsatellites, Central America, DNA, South Florida, urban Miami, Miami, canals, consumers, fish
  • Dataset Contact
    • Position: Information Manager
    • Organization: LTER Network Office
    • Address: UNM Biology Department, MSC03-2020
      1 University of New Mexico
      Albuquerque, NM 87131-0001 USA
    • Phone: 505 277-2535
    • Fax: 505 277-2541
    • Email: tech-support@lternet.edu
    • URL: http://www.lternet.edu

    • Name: Elizabeth Harrison 
    • Organization: Georgia Gwinnet College
    • Address: 1000 University Center Lane
      I-3108
      Lawrenceville, GA 30043 USA
    • Phone: 404-450-8111
    • Email: eharrison1@ggc.edu

    • Position: Information Manager
    • Organization: Florida Coastal Everglades LTER Program
    • Address: Florida International University
      University Park
      OE 148
      Miami, FL 33199 USA
    • Phone: 305-348-6054
    • Fax: 305-348-4096
    • Email: fcelter@fiu.edu
    • URL: http://fcelter.fiu.edu

  • Data Table and Format
    Data Table:  Cichlasoma urophthalmus microsatellite fragment size collected from the Florida Everglades (FCE) and Central America

    Entity Name:
    ST_TDCS_Harrison_001
    Entity Description:
    Cichlasoma urophthalmus microsatellite fragment size collected from the Florida Everglades (FCE) and Central America
    Object Name:
    ST_TDCS_Harrison_001
    Number of Header Lines:
    1
    Attribute Orientation:
    column
    Field Delimiter:
    ,
    Number of Records:
    356