Monthly monitoring fluorescence data for Shark River Slough and Taylor Slough, Everglades National Park (FCE) for October 2004 to February 2014
At a Glance
Jaffe, R.. 2018. Monthly monitoring fluorescence data for Shark River Slough and Taylor Slough, Everglades National Park (FCE) for October 2004 to February 2014. Environmental Data Initiative. https://doi.org/. Dataset accessed 2024-12-04.
Geographic Coverage
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Dataset Creator(s)
- Name: Dr. Rudolf Jaffe
- Position: Lead Principal Investigator
- Organization: Florida Coastal Everglades LTER Program
- Address: Florida International University
University Park
OE 148
Miami, Florida 33199 USA - Phone: 305-348-2456
- Fax: 305-348-4096
- Email: jaffer@fiu.edu
- URL: http://serc.fiu.edu/sercindex/index.htm
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Dataset AbstractDissolved organic matter plays an important role in biogeochemical processes in aquatic environments such as elemental cycling, microbial loop energetics, and the transport of materials across landscapes. Since most of N (> 90%) and P (around 90%) is in the organic form in the oligotrophic subtropical Florida Coastal Everglades (FCE), study of the source and dynamics of dissolved organic matter (DOM) in the ecosystem is crucial for the better understanding of the biogeochemical cycling of nutrients. FCE are composed of estuaries with distinct regions with different biogeochemical processes. Freshwater marsh primarily receives terrestrial input and local autochthonous vegetation production. Mangrove ecotone, nevertheless, is affected by the tidal contributions from Florida Bay and local mangrove production. Florida Bay (FB) is a wedge-shaped shallow oligotrophic estuary which lays south of the Everglades, the bottom of which is covered with a dense biomass of seagrass. The sources of both freshwater and nutrients in FCE are difficult to quantify, owing to the non-point source nature of runoff from the Everglades and the dendritic cross channels in the mangroves. Furthermore, the combination of multiple DOM sources (freshwater marsh vegetation, mangroves, phytoplankton, seagrass, etc.), and the potential seasonal variability of their relative contribution, along with the history of (photo)chemical and microbial diagenetic processing, and complex advective circulation, makes the study of DOM dynamics in FCE particularly difficult using standard schemes of estuarine ecology. Quantitative information of DOM is very useful to investigate the biogeochemical cycling of DOM to a certain degree, however, qualitative information is necessary to better understand the source and dynamics of DOM. Since fluorescence spectroscopic techniques are very sensitive, quick and simple, they have been applied to investigate the fate of DOM in estuaries.
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Geographic CoverageStudy Extent Description
The Study Extent of this dataset includes the Shark River Slough, Taylor Slough and Florida Bay of FCE research sites within Everglades National Park, South Florida
Bounding Coordinates
Samples were collected in FCE-LTER stations
N: 25.761, S: 24.913, E: -80.490, W: -81.078
Florida Coastal Everglades LTER Study Area: South Florida, Everglades National Park, and Florida Bay
N: 25.761, S: 24.913, E: -80.490, W: -81.078
FCE LTER Sites
SRS2, SRS3, SRS4,SRS6, TS/Ph2, TS/Ph3, TS/Ph4, TS/Ph7a
Geographic DescriptionBounding CoordinatesFCE LTER Site SRS2N: 25.550, S: 25.550, E: -80.785, W: -80.785FCE LTER Site SRS3N: 25.468, S: 25.468, E: -80.853, W: -80.853FCE LTER Site SRS4N: 25.410, S: 25.410, E: -80.964, W: -80.964FCE LTER Site SRS6N: 25.365, S: 25.365, E: -81.078, W: -81.078FCE LTER Site TS/Ph2N: 25.40, S: 25.40, E: -80.61, W: -80.61FCE LTER Site TS/Ph3N: 25.25, S: 25.25, E: -80.66, W: -80.66FCE LTER Site TS/Ph4N: 25.32, S: 25.32, E: -80.52, W: -80.52FCE LTER Site TS/Ph7aN: 25.19, S: 25.19, E: -80.64, W: -80.64
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Temporal CoverageStart Date: 2004-10-01
End Date: 2014-02-01
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Attributes
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Data Table: Monthly monitoring fluorescence data for Shark River Slough and Taylor Slough, Everglades National Park (FCE)Attribute Name:Sample_NameAttribute Label:sample nameAttribute Definition:Sample IDStorage Type:textMeasurement Scale:Sample IDMissing Value Code:Attribute Name:SITENAMEAttribute Label:sitenameAttribute Definition:Name of LTER siteStorage Type:textMeasurement Scale:Name of LTER siteMissing Value Code:Attribute Name:DateAttribute Label:dateAttribute Definition:Collection dateStorage Type:datetimeMeasurement Scale:Missing Value Code:Attribute Name:FIAttribute Label:Fluorescence IndexAttribute Definition:Ratio of emission intensities at 450 and 500 nm obtained at a fixedStorage Type:dataMeasurement Scale:Units: dimensionlessPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:A_254Attribute Label:AbsorbanceAttribute Definition:UV-Vis absorbance at 254 nm; note that 1 cm pathlength usedStorage Type:dataMeasurement Scale:Units: dimensionlessPrecision: 0.001
Number Type: realMissing Value Code:-9999.000 (Value will never be recorded )Attribute Name:DOCAttribute Label:Dissolved Organic CarbonAttribute Definition:Dissolved Organic CarbonStorage Type:dataMeasurement Scale:Units: milligramsPerLiterPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:SUVA254Attribute Label:Specific UV absorbanceAttribute Definition:UV absorbance at 254 nm normalized for DOCStorage Type:dataMeasurement Scale:Units: litersPerMeterMilligramPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:Abs_NaperianAttribute Label:Naperian AbsorbanceAttribute Definition:Naperian UV-Vis absorbance at 254 nmStorage Type:dataMeasurement Scale:Units: dimensionlessPrecision: 0.001
Number Type: realMissing Value Code:-9999.000 (Value will never be recorded )Attribute Name:SUVA_NaperianAttribute Label:Naperian Specific UV absorbanceAttribute Definition:Naperian UV absorbance at 254 nm normalized for DOCStorage Type:dataMeasurement Scale:Units: litersPerMeterMilligramPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:C1Attribute Label:C1Attribute Definition:component 1 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: QSUPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:C2Attribute Label:C2Attribute Definition:component 2 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: QSUPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:C3Attribute Label:C3Attribute Definition:component 3 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: QSUPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:C4Attribute Label:C4Attribute Definition:component 4 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: QSUPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:C5Attribute Label:C5Attribute Definition:component 5 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: QSUPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:C6Attribute Label:C6Attribute Definition:component 6 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: QSUPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:C7Attribute Label:C7Attribute Definition:component 7 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: QSUPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:C8Attribute Label:C8Attribute Definition:component 8 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: QSUPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:SUMAttribute Label:Sum of the loadings for all 8 PARAFAC components (AKA total modeled fluorescence intensity)Attribute Definition:Sum of the loadings for all 8 PARAFAC components (AKA total modeled fluorescence intensity)Storage Type:dataMeasurement Scale:Units: QSUPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:%C1Attribute Label:%C1Attribute Definition:component 1 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: percentPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:%C2Attribute Label:%C2Attribute Definition:component 2 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: percentPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:%C3Attribute Label:%C3Attribute Definition:component 3 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: percentPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:%C4Attribute Label:%C4Attribute Definition:component 4 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: percentPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:%C5Attribute Label:%C5Attribute Definition:component 5 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: percentPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:%C6Attribute Label:%C6Attribute Definition:component 6 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: percentPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:%C7Attribute Label:%C7Attribute Definition:component 7 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: percentPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )Attribute Name:%C8Attribute Label:%C8Attribute Definition:component 8 of EEM-PARAFACStorage Type:dataMeasurement Scale:Units: percentPrecision: 0.01
Number Type: realMissing Value Code:-9999.00 (Value will never be recorded )
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Data Table: Monthly monitoring fluorescence data for Shark River Slough and Taylor Slough, Everglades National Park (FCE)
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MethodsSampling Description
Water samples were collected monthly during October 2004 to September 2008 from a total of 17 sampling stations located in the coastal estuaries of the southern tip of the Florida Peninsula, USA. These stations were established for an on-going water quality monitoring program (http://www.serc.fiu.edu/wqmnetwork). Sampling stations can be largely grouped into 3 distinct districts based on the geomorphological features, that is, Florida Bay (FB, 3 sampling stations), Shark River Slough (SRS, 6 sampling stations), and Taylor Slough (TSPH, 8 sampling stations). Surface water samples were taken from the these stations. The samples were collected using pre-washed, brown Nalgen polyethylene bottles (Nalge Nunc International). Salinity of the water samples was measured in the field using an Orion salinity meter. The samples were stored on ice and returned to the laboratory within 8 h for analysis. Subsamples for spectroscopic analysis were filtered through precombusted Whatman GF/F glass fiber filters once received in the laboratory and analyzed immediately.
Method Step
Description
Total organic carbon (TOC) concentrations were analyzed by a high-temperature combustion method with a Shimadzu TOC-5000A TOC analyzer. In advance the analysis, samples were acidified with 3M HCl, and purged with N2 gas to remove inorganic C. Ancillary physical and chemical parameters were measured using standar methods as part of on-going estuarine water quality monitoring program http://www.serc.fiu.edu/wqmnetwork. Detailed methods will be found elsewhere. For escitation-emission matrix (EEM) measurements, fluorescences spectra were measured with a Jobin-Yvon-Horiba (France) Spex Fluoromax-3 fluorometer equipped with a 150-W continuous output xenon arc lamp under condition of 5.7-nm excitation and 2-nm emission slit widths and a 0.25 second response time. Forty-four emission scans were acquired at excitation wavelengths (lamda ex) between 240 and 455 nm at 5 nm intervals. Them emission wavelengths were scanned from lamda ex + 10 nm to lamda ex + 250 nm at 2 nm intervals (Coble et al., 1993 and Coble, 1996). All fluorescence spectra were acquired in ratio mode, whereby the sample (emission signal, S) and reference (excitation lamp output, R) signals were collected and the ratio (S/R) was calculated. The ratio mode eliminates the influence of possible fluctuation and wavelength dependency of excitation lamp output. Several post-acquisition steps were involved in the correction of the fluorescence spectra. First, an inner filter corrections was applied to the fluorescence data according to McKnight et al. (2001). After inner filter corrections the sample EEM underwent spectral subtraction of the Milli-Q water to remove most of the effects due to Raman scattering. Instrument bias related to wavelength dependent efficiencies of the specific instrument's optical components (gratings, mirrors, etc.) were then corrected by applying multiplication factors, supplied by the manufacturer, for both excitation and emission wavelengths for the range of observations. Finally, the fluorescence intensity values were converted to quinine sulfate unit (QSU;1QSU=1 ngL-1 of quinine sulfate monohydroxide) to facilitate inter-laboratory comparisons (Coble et al., 1993). From the 370 nm scan a fluorescence index (FI) was calculated (McKnight et al., 2001). Milli-Q water was used as a reference for all fluorescence analysis. UV-visible measurements of the water samples were carried out with 1cm quartz UV-visible cells at room temperature (20 degrees C), using a Varian CARY 50 Bio UV-visible spectrophotometer. Milli-Q water was used as the reference.
Citation
Battin, T J 1998. Dissolved organic matter and its optical properties in a blackwater tributary of the upper Orinoco river, Venezuela. Organic Geochemistry, 28: 561-569.
Instrumentation
Whatman 0.7um glass fiber filers,Shimadzu TOC-5000A Analyzer,Jobin Yvon Horiba (France) Spex Fluoromax-3 fluorometer, Varian CARY 50 Bio UV visible spectrophotometerMethod Step
Description
Total organic carbon (TOC) concentrations were analyzed by a high-temperature combustion method with a Shimadzu TOC-5000A TOC analyzer. In advance the analysis, samples were acidified with 3M HCl, and purged with N2 gas to remove inorganic C. Ancillary physical and chemical parameters were measured using standar methods as part of on-going estuarine water quality monitoring program http://www.serc.fiu.edu/wqmnetwork. Detailed methods will be found elsewhere. For escitation-emission matrix (EEM) measurements, fluorescences spectra were measured with a Jobin-Yvon-Horiba (France) Spex Fluoromax-3 fluorometer equipped with a 150-W continuous output xenon arc lamp under condition of 5.7-nm excitation and 2-nm emission slit widths and a 0.25 second response time. Forty-four emission scans were acquired at excitation wavelengths (lamda ex) between 240 and 455 nm at 5 nm intervals. Them emission wavelengths were scanned from lamda ex + 10 nm to lamda ex + 250 nm at 2 nm intervals (Coble et al., 1993 and Coble, 1996). All fluorescence spectra were acquired in ratio mode, whereby the sample (emission signal, S) and reference (excitation lamp output, R) signals were collected and the ratio (S/R) was calculated. The ratio mode eliminates the influence of possible fluctuation and wavelength dependency of excitation lamp output. Several post-acquisition steps were involved in the correction of the fluorescence spectra. First, an inner filter corrections was applied to the fluorescence data according to McKnight et al. (2001). After inner filter corrections the sample EEM underwent spectral subtraction of the Milli-Q water to remove most of the effects due to Raman scattering. Instrument bias related to wavelength dependent efficiencies of the specific instrument's optical components (gratings, mirrors, etc.) were then corrected by applying multiplication factors, supplied by the manufacturer, for both excitation and emission wavelengths for the range of observations. Finally, the fluorescence intensity values were converted to quinine sulfate unit (QSU;1QSU=1 ngL-1 of quinine sulfate monohydroxide) to facilitate inter-laboratory comparisons (Coble et al., 1993). From the 370 nm scan a fluorescence index (FI) was calculated (McKnight et al., 2001). Milli-Q water was used as a reference for all fluorescence analysis. UV-visible measurements of the water samples were carried out with 1cm quartz UV-visible cells at room temperature (20 degrees C), using a Varian CARY 50 Bio UV-visible spectrophotometer. Milli-Q water was used as the reference.
Citation
De Souza Sierra, M M 1997. Spectral identification and behavior of dissolved organic fluorescence material during estuarine mixing processes. Marine Chemistry, 58: 51-58.
Instrumentation
Whatman 0.7um glass fiber filers,Shimadzu TOC-5000A Analyzer,Jobin Yvon Horiba (France) Spex Fluoromax-3 fluorometer, Varian CARY 50 Bio UV visible spectrophotometerMethod Step
Description
Total organic carbon (TOC) concentrations were analyzed by a high-temperature combustion method with a Shimadzu TOC-5000A TOC analyzer. In advance the analysis, samples were acidified with 3M HCl, and purged with N2 gas to remove inorganic C. Ancillary physical and chemical parameters were measured using standar methods as part of on-going estuarine water quality monitoring program http://www.serc.fiu.edu/wqmnetwork. Detailed methods will be found elsewhere. For escitation-emission matrix (EEM) measurements, fluorescences spectra were measured with a Jobin-Yvon-Horiba (France) Spex Fluoromax-3 fluorometer equipped with a 150-W continuous output xenon arc lamp under condition of 5.7-nm excitation and 2-nm emission slit widths and a 0.25 second response time. Forty-four emission scans were acquired at excitation wavelengths (lamda ex) between 240 and 455 nm at 5 nm intervals. Them emission wavelengths were scanned from lamda ex + 10 nm to lamda ex + 250 nm at 2 nm intervals (Coble et al., 1993 and Coble, 1996). All fluorescence spectra were acquired in ratio mode, whereby the sample (emission signal, S) and reference (excitation lamp output, R) signals were collected and the ratio (S/R) was calculated. The ratio mode eliminates the influence of possible fluctuation and wavelength dependency of excitation lamp output. Several post-acquisition steps were involved in the correction of the fluorescence spectra. First, an inner filter corrections was applied to the fluorescence data according to McKnight et al. (2001). After inner filter corrections the sample EEM underwent spectral subtraction of the Milli-Q water to remove most of the effects due to Raman scattering. Instrument bias related to wavelength dependent efficiencies of the specific instrument's optical components (gratings, mirrors, etc.) were then corrected by applying multiplication factors, supplied by the manufacturer, for both excitation and emission wavelengths for the range of observations. Finally, the fluorescence intensity values were converted to quinine sulfate unit (QSU;1QSU=1 ngL-1 of quinine sulfate monohydroxide) to facilitate inter-laboratory comparisons (Coble et al., 1993). From the 370 nm scan a fluorescence index (FI) was calculated (McKnight et al., 2001). Milli-Q water was used as a reference for all fluorescence analysis. UV-visible measurements of the water samples were carried out with 1cm quartz UV-visible cells at room temperature (20 degrees C), using a Varian CARY 50 Bio UV-visible spectrophotometer. Milli-Q water was used as the reference.
Citation
Donard, O F 1989. High-sensitivity fluorescence spectroscopy of Mediterranean waters using a conventional or a pulsed laser excitation source. Marine Chemistry, 27: 117-136.
Instrumentation
Whatman 0.7um glass fiber filers,Shimadzu TOC-5000A Analyzer,Jobin Yvon Horiba (France) Spex Fluoromax-3 fluorometer, Varian CARY 50 Bio UV visible spectrophotometerMethod Step
Description
Total organic carbon (TOC) concentrations were analyzed by a high-temperature combustion method with a Shimadzu TOC-5000A TOC analyzer. In advance the analysis, samples were acidified with 3M HCl, and purged with N2 gas to remove inorganic C. Ancillary physical and chemical parameters were measured using standar methods as part of on-going estuarine water quality monitoring program http://www.serc.fiu.edu/wqmnetwork. Detailed methods will be found elsewhere. For escitation-emission matrix (EEM) measurements, fluorescences spectra were measured with a Jobin-Yvon-Horiba (France) Spex Fluoromax-3 fluorometer equipped with a 150-W continuous output xenon arc lamp under condition of 5.7-nm excitation and 2-nm emission slit widths and a 0.25 second response time. Forty-four emission scans were acquired at excitation wavelengths (lamda ex) between 240 and 455 nm at 5 nm intervals. Them emission wavelengths were scanned from lamda ex + 10 nm to lamda ex + 250 nm at 2 nm intervals (Coble et al., 1993 and Coble, 1996). All fluorescence spectra were acquired in ratio mode, whereby the sample (emission signal, S) and reference (excitation lamp output, R) signals were collected and the ratio (S/R) was calculated. The ratio mode eliminates the influence of possible fluctuation and wavelength dependency of excitation lamp output. Several post-acquisition steps were involved in the correction of the fluorescence spectra. First, an inner filter corrections was applied to the fluorescence data according to McKnight et al. (2001). After inner filter corrections the sample EEM underwent spectral subtraction of the Milli-Q water to remove most of the effects due to Raman scattering. Instrument bias related to wavelength dependent efficiencies of the specific instrument's optical components (gratings, mirrors, etc.) were then corrected by applying multiplication factors, supplied by the manufacturer, for both excitation and emission wavelengths for the range of observations. Finally, the fluorescence intensity values were converted to quinine sulfate unit (QSU;1QSU=1 ngL-1 of quinine sulfate monohydroxide) to facilitate inter-laboratory comparisons (Coble et al., 1993). From the 370 nm scan a fluorescence index (FI) was calculated (McKnight et al., 2001). Milli-Q water was used as a reference for all fluorescence analysis. UV-visible measurements of the water samples were carried out with 1cm quartz UV-visible cells at room temperature (20 degrees C), using a Varian CARY 50 Bio UV-visible spectrophotometer. Milli-Q water was used as the reference.
Citation
Lu, X Q 2003. Molecular characterization of dissolved organic matter in freshwater wetlands of the Florida Everglades. Water Research, 37: 2599-2606.
Instrumentation
Whatman 0.7um glass fiber filers,Shimadzu TOC-5000A Analyzer,Jobin Yvon Horiba (France) Spex Fluoromax-3 fluorometer, Varian CARY 50 Bio UV visible spectrophotometerMethod Step
Description
Total organic carbon (TOC) concentrations were analyzed by a high-temperature combustion method with a Shimadzu TOC-5000A TOC analyzer. In advance the analysis, samples were acidified with 3M HCl, and purged with N2 gas to remove inorganic C. Ancillary physical and chemical parameters were measured using standar methods as part of on-going estuarine water quality monitoring program http://www.serc.fiu.edu/wqmnetwork. Detailed methods will be found elsewhere. For escitation-emission matrix (EEM) measurements, fluorescences spectra were measured with a Jobin-Yvon-Horiba (France) Spex Fluoromax-3 fluorometer equipped with a 150-W continuous output xenon arc lamp under condition of 5.7-nm excitation and 2-nm emission slit widths and a 0.25 second response time. Forty-four emission scans were acquired at excitation wavelengths (lamda ex) between 240 and 455 nm at 5 nm intervals. Them emission wavelengths were scanned from lamda ex + 10 nm to lamda ex + 250 nm at 2 nm intervals (Coble et al., 1993 and Coble, 1996). All fluorescence spectra were acquired in ratio mode, whereby the sample (emission signal, S) and reference (excitation lamp output, R) signals were collected and the ratio (S/R) was calculated. The ratio mode eliminates the influence of possible fluctuation and wavelength dependency of excitation lamp output. Several post-acquisition steps were involved in the correction of the fluorescence spectra. First, an inner filter corrections was applied to the fluorescence data according to McKnight et al. (2001). After inner filter corrections the sample EEM underwent spectral subtraction of the Milli-Q water to remove most of the effects due to Raman scattering. Instrument bias related to wavelength dependent efficiencies of the specific instrument's optical components (gratings, mirrors, etc.) were then corrected by applying multiplication factors, supplied by the manufacturer, for both excitation and emission wavelengths for the range of observations. Finally, the fluorescence intensity values were converted to quinine sulfate unit (QSU;1QSU=1 ngL-1 of quinine sulfate monohydroxide) to facilitate inter-laboratory comparisons (Coble et al., 1993). From the 370 nm scan a fluorescence index (FI) was calculated (McKnight et al., 2001). Milli-Q water was used as a reference for all fluorescence analysis. UV-visible measurements of the water samples were carried out with 1cm quartz UV-visible cells at room temperature (20 degrees C), using a Varian CARY 50 Bio UV-visible spectrophotometer. Milli-Q water was used as the reference.
Citation
McKnight, Diane M 2001. Spectrofluorometric characterization of dissolved organic matter for indication of precursor organic material and aromaticity. Limnology and Oceanography, 46: 38-48.
Instrumentation
Whatman 0.7um glass fiber filers,Shimadzu TOC-5000A Analyzer,Jobin Yvon Horiba (France) Spex Fluoromax-3 fluorometer, Varian CARY 50 Bio UV visible spectrophotometerMethod Step
Description
Total organic carbon (TOC) concentrations were analyzed by a high-temperature combustion method with a Shimadzu TOC-5000A TOC analyzer. In advance the analysis, samples were acidified with 3M HCl, and purged with N2 gas to remove inorganic C. Ancillary physical and chemical parameters were measured using standar methods as part of on-going estuarine water quality monitoring program http://www.serc.fiu.edu/wqmnetwork. Detailed methods will be found elsewhere. For escitation-emission matrix (EEM) measurements, fluorescences spectra were measured with a Jobin-Yvon-Horiba (France) Spex Fluoromax-3 fluorometer equipped with a 150-W continuous output xenon arc lamp under condition of 5.7-nm excitation and 2-nm emission slit widths and a 0.25 second response time. Forty-four emission scans were acquired at excitation wavelengths (lamda ex) between 240 and 455 nm at 5 nm intervals. Them emission wavelengths were scanned from lamda ex + 10 nm to lamda ex + 250 nm at 2 nm intervals (Coble et al., 1993 and Coble, 1996). All fluorescence spectra were acquired in ratio mode, whereby the sample (emission signal, S) and reference (excitation lamp output, R) signals were collected and the ratio (S/R) was calculated. The ratio mode eliminates the influence of possible fluctuation and wavelength dependency of excitation lamp output. Several post-acquisition steps were involved in the correction of the fluorescence spectra. First, an inner filter corrections was applied to the fluorescence data according to McKnight et al. (2001). After inner filter corrections the sample EEM underwent spectral subtraction of the Milli-Q water to remove most of the effects due to Raman scattering. Instrument bias related to wavelength dependent efficiencies of the specific instrument's optical components (gratings, mirrors, etc.) were then corrected by applying multiplication factors, supplied by the manufacturer, for both excitation and emission wavelengths for the range of observations. Finally, the fluorescence intensity values were converted to quinine sulfate unit (QSU;1QSU=1 ngL-1 of quinine sulfate monohydroxide) to facilitate inter-laboratory comparisons (Coble et al., 1993). From the 370 nm scan a fluorescence index (FI) was calculated (McKnight et al., 2001). Milli-Q water was used as a reference for all fluorescence analysis. UV-visible measurements of the water samples were carried out with 1cm quartz UV-visible cells at room temperature (20 degrees C), using a Varian CARY 50 Bio UV-visible spectrophotometer. Milli-Q water was used as the reference.
Citation
Helms, John R. 2008. Absorption spectral slopes and slope ratios as indicators of molecular weight, source, and photobleaching of chromophoric dissolved organic matter. Limnology and Oceanography, 53(3): 955-969.
Instrumentation
Whatman 0.7um glass fiber filers,Shimadzu TOC-5000A Analyzer,Jobin Yvon Horiba (France) Spex Fluoromax-3 fluorometer, Varian CARY 50 Bio UV visible spectrophotometerMethod Step
Description
Total organic carbon (TOC) concentrations were analyzed by a high-temperature combustion method with a Shimadzu TOC-5000A TOC analyzer. In advance the analysis, samples were acidified with 3M HCl, and purged with N2 gas to remove inorganic C. Ancillary physical and chemical parameters were measured using standar methods as part of on-going estuarine water quality monitoring program http://www.serc.fiu.edu/wqmnetwork. Detailed methods will be found elsewhere. For escitation-emission matrix (EEM) measurements, fluorescences spectra were measured with a Jobin-Yvon-Horiba (France) Spex Fluoromax-3 fluorometer equipped with a 150-W continuous output xenon arc lamp under condition of 5.7-nm excitation and 2-nm emission slit widths and a 0.25 second response time. Forty-four emission scans were acquired at excitation wavelengths (lamda ex) between 240 and 455 nm at 5 nm intervals. Them emission wavelengths were scanned from lamda ex + 10 nm to lamda ex + 250 nm at 2 nm intervals (Coble et al., 1993 and Coble, 1996). All fluorescence spectra were acquired in ratio mode, whereby the sample (emission signal, S) and reference (excitation lamp output, R) signals were collected and the ratio (S/R) was calculated. The ratio mode eliminates the influence of possible fluctuation and wavelength dependency of excitation lamp output. Several post-acquisition steps were involved in the correction of the fluorescence spectra. First, an inner filter corrections was applied to the fluorescence data according to McKnight et al. (2001). After inner filter corrections the sample EEM underwent spectral subtraction of the Milli-Q water to remove most of the effects due to Raman scattering. Instrument bias related to wavelength dependent efficiencies of the specific instrument's optical components (gratings, mirrors, etc.) were then corrected by applying multiplication factors, supplied by the manufacturer, for both excitation and emission wavelengths for the range of observations. Finally, the fluorescence intensity values were converted to quinine sulfate unit (QSU;1QSU=1 ngL-1 of quinine sulfate monohydroxide) to facilitate inter-laboratory comparisons (Coble et al., 1993). From the 370 nm scan a fluorescence index (FI) was calculated (McKnight et al., 2001). Milli-Q water was used as a reference for all fluorescence analysis. UV-visible measurements of the water samples were carried out with 1cm quartz UV-visible cells at room temperature (20 degrees C), using a Varian CARY 50 Bio UV-visible spectrophotometer. Milli-Q water was used as the reference.
Citation
Weishaar, James L. 2003. Evaluation of Specific Ultraviolet Absorbance as an Indicator of the Chemical Composition and Reactivity of Dissolved Organic Carbon . Environmental Science and Technology, 37: 4702-4708.
Instrumentation
Whatman 0.7um glass fiber filers,Shimadzu TOC-5000A Analyzer,Jobin Yvon Horiba (France) Spex Fluoromax-3 fluorometer, Varian CARY 50 Bio UV visible spectrophotometerMethod Step
Description
Total organic carbon (TOC) concentrations were analyzed by a high-temperature combustion method with a Shimadzu TOC-5000A TOC analyzer. In advance the analysis, samples were acidified with 3M HCl, and purged with N2 gas to remove inorganic C. Ancillary physical and chemical parameters were measured using standar methods as part of on-going estuarine water quality monitoring program http://www.serc.fiu.edu/wqmnetwork. Detailed methods will be found elsewhere. For escitation-emission matrix (EEM) measurements, fluorescences spectra were measured with a Jobin-Yvon-Horiba (France) Spex Fluoromax-3 fluorometer equipped with a 150-W continuous output xenon arc lamp under condition of 5.7-nm excitation and 2-nm emission slit widths and a 0.25 second response time. Forty-four emission scans were acquired at excitation wavelengths (lamda ex) between 240 and 455 nm at 5 nm intervals. Them emission wavelengths were scanned from lamda ex + 10 nm to lamda ex + 250 nm at 2 nm intervals (Coble et al., 1993 and Coble, 1996). All fluorescence spectra were acquired in ratio mode, whereby the sample (emission signal, S) and reference (excitation lamp output, R) signals were collected and the ratio (S/R) was calculated. The ratio mode eliminates the influence of possible fluctuation and wavelength dependency of excitation lamp output. Several post-acquisition steps were involved in the correction of the fluorescence spectra. First, an inner filter corrections was applied to the fluorescence data according to McKnight et al. (2001). After inner filter corrections the sample EEM underwent spectral subtraction of the Milli-Q water to remove most of the effects due to Raman scattering. Instrument bias related to wavelength dependent efficiencies of the specific instrument's optical components (gratings, mirrors, etc.) were then corrected by applying multiplication factors, supplied by the manufacturer, for both excitation and emission wavelengths for the range of observations. Finally, the fluorescence intensity values were converted to quinine sulfate unit (QSU;1QSU=1 ngL-1 of quinine sulfate monohydroxide) to facilitate inter-laboratory comparisons (Coble et al., 1993). From the 370 nm scan a fluorescence index (FI) was calculated (McKnight et al., 2001). Milli-Q water was used as a reference for all fluorescence analysis. UV-visible measurements of the water samples were carried out with 1cm quartz UV-visible cells at room temperature (20 degrees C), using a Varian CARY 50 Bio UV-visible spectrophotometer. Milli-Q water was used as the reference.
Citation
Stedmon, Colin A. 2003. Tracing dissolved organic matter in aquatic environments using a new approach to fluorescence spectroscopy . Marine Chemistry, 82: 239-254.
Instrumentation
Whatman 0.7um glass fiber filers,Shimadzu TOC-5000A Analyzer,Jobin Yvon Horiba (France) Spex Fluoromax-3 fluorometer, Varian CARY 50 Bio UV visible spectrophotometerMethod Step
Description
Total organic carbon (TOC) concentrations were analyzed by a high-temperature combustion method with a Shimadzu TOC-5000A TOC analyzer. In advance the analysis, samples were acidified with 3M HCl, and purged with N2 gas to remove inorganic C. Ancillary physical and chemical parameters were measured using standar methods as part of on-going estuarine water quality monitoring program http://www.serc.fiu.edu/wqmnetwork. Detailed methods will be found elsewhere. For escitation-emission matrix (EEM) measurements, fluorescences spectra were measured with a Jobin-Yvon-Horiba (France) Spex Fluoromax-3 fluorometer equipped with a 150-W continuous output xenon arc lamp under condition of 5.7-nm excitation and 2-nm emission slit widths and a 0.25 second response time. Forty-four emission scans were acquired at excitation wavelengths (lamda ex) between 240 and 455 nm at 5 nm intervals. Them emission wavelengths were scanned from lamda ex + 10 nm to lamda ex + 250 nm at 2 nm intervals (Coble et al., 1993 and Coble, 1996). All fluorescence spectra were acquired in ratio mode, whereby the sample (emission signal, S) and reference (excitation lamp output, R) signals were collected and the ratio (S/R) was calculated. The ratio mode eliminates the influence of possible fluctuation and wavelength dependency of excitation lamp output. Several post-acquisition steps were involved in the correction of the fluorescence spectra. First, an inner filter corrections was applied to the fluorescence data according to McKnight et al. (2001). After inner filter corrections the sample EEM underwent spectral subtraction of the Milli-Q water to remove most of the effects due to Raman scattering. Instrument bias related to wavelength dependent efficiencies of the specific instrument's optical components (gratings, mirrors, etc.) were then corrected by applying multiplication factors, supplied by the manufacturer, for both excitation and emission wavelengths for the range of observations. Finally, the fluorescence intensity values were converted to quinine sulfate unit (QSU;1QSU=1 ngL-1 of quinine sulfate monohydroxide) to facilitate inter-laboratory comparisons (Coble et al., 1993). From the 370 nm scan a fluorescence index (FI) was calculated (McKnight et al., 2001). Milli-Q water was used as a reference for all fluorescence analysis. UV-visible measurements of the water samples were carried out with 1cm quartz UV-visible cells at room temperature (20 degrees C), using a Varian CARY 50 Bio UV-visible spectrophotometer. Milli-Q water was used as the reference.
Citation
Chen, Meilian L. 2010. Comparative study of dissolved organic matter from groundwater and surface water in the Florida costal Everglades using multi-dimensional spectrofluorometry combined with multivariate statistics. Applied Geochemistry, 25: 872-880.
Instrumentation
Whatman 0.7um glass fiber filers,Shimadzu TOC-5000A Analyzer,Jobin Yvon Horiba (France) Spex Fluoromax-3 fluorometer, Varian CARY 50 Bio UV visible spectrophotometerMethod Step
Description
Total organic carbon (TOC) concentrations were analyzed by a high-temperature combustion method with a Shimadzu TOC-5000A TOC analyzer. In advance the analysis, samples were acidified with 3M HCl, and purged with N2 gas to remove inorganic C. Ancillary physical and chemical parameters were measured using standar methods as part of on-going estuarine water quality monitoring program http://www.serc.fiu.edu/wqmnetwork. Detailed methods will be found elsewhere. For escitation-emission matrix (EEM) measurements, fluorescences spectra were measured with a Jobin-Yvon-Horiba (France) Spex Fluoromax-3 fluorometer equipped with a 150-W continuous output xenon arc lamp under condition of 5.7-nm excitation and 2-nm emission slit widths and a 0.25 second response time. Forty-four emission scans were acquired at excitation wavelengths (lamda ex) between 240 and 455 nm at 5 nm intervals. Them emission wavelengths were scanned from lamda ex + 10 nm to lamda ex + 250 nm at 2 nm intervals (Coble et al., 1993 and Coble, 1996). All fluorescence spectra were acquired in ratio mode, whereby the sample (emission signal, S) and reference (excitation lamp output, R) signals were collected and the ratio (S/R) was calculated. The ratio mode eliminates the influence of possible fluctuation and wavelength dependency of excitation lamp output. Several post-acquisition steps were involved in the correction of the fluorescence spectra. First, an inner filter corrections was applied to the fluorescence data according to McKnight et al. (2001). After inner filter corrections the sample EEM underwent spectral subtraction of the Milli-Q water to remove most of the effects due to Raman scattering. Instrument bias related to wavelength dependent efficiencies of the specific instrument's optical components (gratings, mirrors, etc.) were then corrected by applying multiplication factors, supplied by the manufacturer, for both excitation and emission wavelengths for the range of observations. Finally, the fluorescence intensity values were converted to quinine sulfate unit (QSU;1QSU=1 ngL-1 of quinine sulfate monohydroxide) to facilitate inter-laboratory comparisons (Coble et al., 1993). From the 370 nm scan a fluorescence index (FI) was calculated (McKnight et al., 2001). Milli-Q water was used as a reference for all fluorescence analysis. UV-visible measurements of the water samples were carried out with 1cm quartz UV-visible cells at room temperature (20 degrees C), using a Varian CARY 50 Bio UV-visible spectrophotometer. Milli-Q water was used as the reference.
Citation
Yamashita, Youhei 2010. Dissolved organic matter characteristics across a subtropical wetland's landscape: Application of optical properties in the assessment of environmental dynamics. Ecosystems, 13: 1006-1019.
Instrumentation
Whatman 0.7um glass fiber filers,Shimadzu TOC-5000A Analyzer,Jobin Yvon Horiba (France) Spex Fluoromax-3 fluorometer, Varian CARY 50 Bio UV visible spectrophotometerQuality Control
Fluorescence measurements are corrected for internal absorbance quenching. Fluorescence spectra are corrected for internal instrument configuration using excitation and emission correction factors. For DOC, Humic carbon and carbohydrate data, we create calibration curves with standards and then graph the data.
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Distribution and Intellectual RightsOnline distribution
http://fcelter.fiu.edu/perl/public_data_download.pl?datasetid=LT_ND_Jaffe_004.txt
Data Submission Date: 2015-07-29
Intellectual Rights
These data are classified as 'Type II' whereby original FCE LTER experimental data collected by individual FCE researchers to be released to restricted audiences according to terms specified by the owners of the data. Type II data are considered to be exceptional and should be rare in occurrence. The justification for exceptions must be well documented and approved by the lead PI and Site Data Manager. Some examples of Type II data restrictions may include: locations of rare or endangered species, data that are covered under prior licensing or copyright (e.g., SPOT satellite data), or covered by the Human Subjects Act, Student Dissertation data and those data related to the FCE LTER Program but not funded by the National Science Foundation (NSF) under LTER grants #DEB-9910514, and # DBI-0620409. Researchers that make use of Type II Data may be subject to additional restrictions to protect any applicable commercial or confidentiality interests. All publications based on this dataset must cite the data Contributor, the Florida Coastal Everglades Long-Term Ecological Research (LTER) Program and that this material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DEB-1237517, #DBI-0620409, and #DEB-9910514. Additionally, two copies of the manuscript must be submitted to the Florida Coastal Everglades LTER Program Office, LTER Program Manager, Florida International University, Southeast Environmental Research Center, OE 148, University Park, Miami, Florida 33199. For a complete description of the FCE LTER Data Access Policy and Data User Agreement, please go to FCE Data Management Policy at http://fcelter.fiu.edu/data/DataMgmt.pdf and LTER Network Data Access Policy at http://fcelter.fiu.edu/data/core/data_user_agreement/distribution_policy.html.
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Publications citing this datasetRegier, Peter, Laurel Larsen, Kaelin Cawley, and Rudolf Jaffé 2024. Linking Hydrology and Dissolved Organic Matter Characteristics in a Subtropical Wetland: A Long-Term Study of the Florida Everglades. Global Biogeochemical Cycles 34:
DOI : 10.1029/2020GB006648
Zeller, Mary A., Bryce R. Van Dam, Christian Lopes, and John S. Kominoski 2024. Carbonate-Associated Organic Matter Is a Detectable Dissolved Organic Matter Source in a Subtropical Seagrass Meadow. Front. Mar. Sci. 7:
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KeywordsFCE, Florida Coastal Everglades LTER, ecological research, long-term monitoring, Everglades National Park, Dissolved organic matter, Taylor Slough, Shark River Slough, Fluorescence Index, Absorbance, Specific UV absorbance, fluorescence, water, total organic carbon, estuarine, sulfate, emissions
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Dataset Contact
- Name: Rudolf Jaffe
- Position: Project Collaborator
- Organization: Florida Coastal Everglades LTER Program
- Address: Florida International University
University Park
OE 148
Miami, Florida 33199 USA - Phone: 305-348-2456
- Fax: 305-348-4096
- Email: jaffer@fiu.edu
- URL: http://serc.fiu.edu/sercindex/index.htm
- Position: Information Manager
- Organization: Florida Coastal Everglades LTER Program
- Address: Florida International University
University Park
OE 148
Miami, FL 33199 USA - Phone: 305-348-6054
- Fax: 305-348-4096
- Email: fcelter@fiu.edu
- URL: http://fcelter.fiu.edu
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Data Table and FormatData Table: Monthly monitoring fluorescence data for Shark River Slough and Taylor Slough, Everglades National Park (FCE)Entity Name:LT_ND_Jaffe_004Entity Description:Monthly monitoring fluorescence data for Shark River Slough and Taylor Slough, Everglades National Park (FCE)Object Name:LT_ND_Jaffe_004Number of Header Lines:1Attribute Orientation:columnField Delimiter:,Number of Records:697
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Metadata Provider
- Organization: Florida Coastal Everglades LTER Program
- Address: Florida International University
University Park
OE 148
Miami, FL 33199 USA - Phone: 305-348-6054
- Email: fcelter@fiu.edu
- URL: http://fcelter.fiu.edu