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Overnight Shark River Surveys from Shark River Slough, Everglades National Park (FCE), South Florida from October 2001 to March 2002


At a Glance


Authors: Joseph Boyer, Susan Dailey
Time period: 2001-10-01 to 2002-03-12
Package id: knb-lter-fce.1068.3
Dataset id: LT_ND_Dailey_002

How to cite:
Boyer, J., S. Dailey. 2003. Overnight Shark River Surveys from Shark River Slough, Everglades National Park (FCE), South Florida from October 2001 to March 2002. Environmental Data Initiative. https://doi.org/10.6073/pasta/8b6e429fb37dbeaeaa22f962af725a42. Dataset accessed 2020-08-08.

Geographic Coverage


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Detailed Metadata


  • Dataset Abstract
    Estuarine mixing interfaces have been a focus of wetland ecologists for the last three decades and are of growing concern today. Particularly critical in Everglades restoration, water quality and the dynamics of nutrients and dissolved materials across seasons in the natural system are being defined. We measured diurnal and seasonal (wet and dry) variation in microbial, dissolved and total nutrients and total organic carbon along a riverine transect and for a diurnal cycle in October of 2001 and March of 2002. For the diurnal sampling we anchored a houseboat mid Shark River 100m up river of the mesohaline Florida Coastal Everglades Shark River LTER site and sampled the water for chemical, microbial, and physical parameters. At slack high and low tides we sampled 10 miles offshore then at three sites up the Shark River. TOC (mM) and salinity (ppt) were highly correlated (r2 .97) along the river transect across seasons and TOC derived from terrestrial input. Bacterial production ranged from 1.88 to 10.99 mg C l-1 d-1 and was greatest in the dry season and at the most freshwater sampling points. Combined examination of our 49 sampled parameters both from the diurnal sampling and river transect sampling showed that our position for sampling in the river was critical and that the mid river-estuarine productivity maxima occurred within Shark River in both the wet and dry season. These data suggest this oligotrophic estuarine-river system is highly sensitive to watershed and river fringe nutrient inputs and remineralization vectors within the river.
  • Geographic Coverage
    Study Extent Description
    The Study Extent of this dataset includes the FCE Shark River Slough and Taylor Slough research sites within Everglades National Park, South Florida

    Bounding Coordinates
    Data were collected in the Shark River Slough, within Everglades National Park, South Florida
    N: 25.410, S: 25.365, E: -80.964, W: -81.078

    Florida Coastal Everglades LTER Study Area: South Florida, Everglades National Park, and Florida Bay
    N: 25.761, S: 24.913, E: -80.490, W: -81.078

    FCE LTER Sites
    SRS4, SRS5, SRS6

    All Sites
    Geographic Description
    Bounding Coordinates
    FCE LTER Site SRS4
    N: 25.410, S: 25.410, E: -80.964, W: -80.964
    FCE LTER Site SRS5
    N: 25.377, S: 25.377, E: -81.032, W: -81.032
    FCE LTER Site SRS6
    N: 25.365, S: 25.365, E: -81.078, W: -81.078
  • Attributes
    • Data Table:   Overnight Shark River Surveys from Shark River Slough, Everglades National Park (FCE), South Florida
      Attribute Name:
      SITENAME
      Attribute Label:
      sitename
      Attribute Definition:
      Name of LTER site
      Storage Type:
      text
      Measurement Scale:
      Name of LTER site
      Missing Value Code:
       

      Attribute Name:
      ID Number
      Attribute Label:
      ID Number
      Attribute Definition:
      Sample ID Number
      Storage Type:
      text
      Measurement Scale:
      Sample ID Number
      Missing Value Code:
       

      Attribute Name:
      Date
      Attribute Label:
      date
      Attribute Definition:
      Sample Collection Date
      Storage Type:
      datetime
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      Tide
      Attribute Label:
      stage
      Attribute Definition:
      Tidal Stage
      Storage Type:
      text
      Measurement Scale:
      Tidal Stage
      Missing Value Code:
       

      Attribute Name:
      Time
      Attribute Label:
      time
      Attribute Definition:
      Collection Time
      Storage Type:
      datetime
      Measurement Scale:
      Missing Value Code:
       

      Attribute Name:
      TN
      Attribute Label:
      concentration
      Attribute Definition:
      Total Nitrogen in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microMolesPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      TON
      Attribute Label:
      concentration
      Attribute Definition:
      Total Organic Nitrogen in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microMolesPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      TOC
      Attribute Label:
      concentration
      Attribute Definition:
      Total Organic Carbon in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microMolesPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      NOx
      Attribute Label:
      concentration
      Attribute Definition:
      Nitrate + Nitrite in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microMolesPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      NO3
      Attribute Label:
      concentration
      Attribute Definition:
      Nitrate in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microMolesPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      NO2
      Attribute Label:
      concentration
      Attribute Definition:
      Nitrite in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microMolesPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      NH4
      Attribute Label:
      concentration
      Attribute Definition:
      Ammonium in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microMolesPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      SRP
      Attribute Label:
      concentration
      Attribute Definition:
      Soluble Reactive Phosphorus in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microMolesPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      TP
      Attribute Label:
      concentration
      Attribute Definition:
      Total Phosphorus in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microMolesPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      Si
      Attribute Label:
      concentration
      Attribute Definition:
      Silica in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microMolesPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      Turb
      Attribute Label:
      Turbidity
      Attribute Definition:
      Turbidity
      Storage Type:
      data
      Measurement Scale:
      Units: dimensionless
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      APA
      Attribute Label:
      concentration
      Attribute Definition:
      Alkaline Phosphatase Activity in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microMolesPerLiterPerHour
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      CHLA
      Attribute Label:
      concentration
      Attribute Definition:
      Chlorophyll a in Water
      Storage Type:
      data
      Measurement Scale:
      Units: microgramsPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      SAL-S
      Attribute Label:
      salinity
      Attribute Definition:
      Water Surface Salinity
      Storage Type:
      data
      Measurement Scale:
      Units: PSU
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )

      Attribute Name:
      SAL-B
      Attribute Label:
      salinity
      Attribute Definition:
      Water Bottom Salinity
      Storage Type:
      data
      Measurement Scale:
      Units: PSU
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )

      Attribute Name:
      DO-S
      Attribute Label:
      concentration
      Attribute Definition:
      Water Surface Dissolved Oxygen
      Storage Type:
      data
      Measurement Scale:
      Units: milligramsPerLiter
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )

      Attribute Name:
      DO-B
      Attribute Label:
      concentration
      Attribute Definition:
      Water Bottom Dissolved Oxygen
      Storage Type:
      data
      Measurement Scale:
      Units: milligramsPerLiter
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )

      Attribute Name:
      TEMP-S
      Attribute Label:
      temperature
      Attribute Definition:
      Water Surface Temperature
      Storage Type:
      data
      Measurement Scale:
      Units: celsius
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )

      Attribute Name:
      TEMP-B
      Attribute Label:
      temperature
      Attribute Definition:
      Water Bottom Temperature
      Storage Type:
      data
      Measurement Scale:
      Units: celsius
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )

      Attribute Name:
      BacteriaAbundance
      Attribute Label:
      abundance
      Attribute Definition:
      Bacteria Abundance
      Storage Type:
      data
      Measurement Scale:
      Units: numberPerMilliliter
      Precision: 1
      Number Type: real
      Missing Value Code:
      -9999 (Value will never be recorded )

      Attribute Name:
      BacteriaProductionDailyRate
      Attribute Label:
      rate
      Attribute Definition:
      Bacteria Daily Production
      Storage Type:
      data
      Measurement Scale:
      Units: microgramsPerLiterPerDay
      Precision: 0.0001
      Number Type: real
      Missing Value Code:
      -9999.0000 (Value will never be recorded )

      Attribute Name:
      Chl_a_Blue
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Chl a Blue
      Storage Type:
      data
      Measurement Scale:
      Units: microgramsPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      Chl_a_Green
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Chl a Green
      Storage Type:
      data
      Measurement Scale:
      Units: microgramsPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      Chl_a_Brown
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Chl a Brown
      Storage Type:
      data
      Measurement Scale:
      Units: microgramsPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      Sum_Chl_a_PAM
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Sum Chl a PAM
      Storage Type:
      data
      Measurement Scale:
      Units: microgramsPerLiter
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      QuantumYield_SUM
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Quantum Yield SUM
      Storage Type:
      data
      Measurement Scale:
      Units: dimensionless
      Precision: 0.01
      Number Type: real
      Missing Value Code:
      -9999.00 (Value will never be recorded )

      Attribute Name:
      AlphaBlue
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Algal Energetics Light Curve Slope Cyanobacteria
      Storage Type:
      data
      Measurement Scale:
      Units: dimensionless
      Precision: 0.001
      Number Type: real
      Missing Value Code:
      -9999.000 (Value will never be recorded )

      Attribute Name:
      AlphaGreen
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Algal Energetics Light Curve Slope Brown
      Storage Type:
      data
      Measurement Scale:
      Units: dimensionless
      Precision: 0.001
      Number Type: real
      Missing Value Code:
      -9999.000 (Value will never be recorded )

      Attribute Name:
      AlphaBrown
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Algal Energetics Light Curve Slope Green
      Storage Type:
      data
      Measurement Scale:
      Units: dimensionless
      Precision: 0.001
      Number Type: real
      Missing Value Code:
      -9999.000 (Value will never be recorded )

      Attribute Name:
      ETRmax_Blue
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Electron Transport Rate Maximum Cyanobacteria
      Storage Type:
      data
      Measurement Scale:
      Units: dimensionless
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )

      Attribute Name:
      ETRmax_Green
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Electron Transport Rate Maximum Green
      Storage Type:
      data
      Measurement Scale:
      Units: dimensionless
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )

      Attribute Name:
      ETRmax_Brown
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Electron Transport Rate Maximum Brown
      Storage Type:
      data
      Measurement Scale:
      Units: dimensionless
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )

      Attribute Name:
      Ik_Blue
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Initial Slope Irradiance Maximum Cyanobacteria
      Storage Type:
      data
      Measurement Scale:
      Units: dimensionless
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )

      Attribute Name:
      Ik_Green
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Initial Slope Irradiance Maximum Brown
      Storage Type:
      data
      Measurement Scale:
      Units: dimensionless
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )

      Attribute Name:
      Ik_Brown
      Attribute Label:
      PAM fluorometry
      Attribute Definition:
      Initial Slope Irradiance Maximum Green
      Storage Type:
      data
      Measurement Scale:
      Units: dimensionless
      Precision: 0.1
      Number Type: real
      Missing Value Code:
      -9999.0 (Value will never be recorded )


  • Methods
    Sampling Description
    Water column samples collected 10 cm below water surface with 3 rinses with sample water.

    Method Step

    Description
    Dissolved and total nutrient analyses-Samples for total analyses were kept on ice and transported to the lab where they were refrigerated at 4 degrees C then analyzed within 28 days of collection (TOC, TN, TP, Tsi). Samples for dissolved constituents (NH4 NO3, NO2, N+N, DOC, SRP) were immediately processed in the lab as well as samples for Chl a, direct counts of bacteria, total nutrients (TOC, TN, TP), APA, and bacterial production. A sample for PAM analysis was set aside for a 10 hr dark acclimation period. Microbial Analyses-We collected duplicate samples at each sampling interval, processed and analyzed them to determine bacterial abundance, algal energetics and chl a by phytoplankton guild, enzyme activity, and heterotrophic bacterial production. Samples for microbial analyses were collected in washed, acid-rinsed, ADDIH2O rinsed, then sample rinsed brown, 250 ml, Nalgene bottles. Depth of sampling was 10 cm below the water surface. Bacterial abundance was determined through epifluorescence microscopy using DAPI DNA stain after preservation with 2% bacterial free phosphate buffered formalin and dark storage. Slides were made within 14 days of collection and counted immediately after they were prepared. Algal dynamics were determined through PAM (pulse-amplitude modulation) fluorescence within 24 hours of collection. Algal energetics samples were analyzed using PAM fluormetry for Chl a content and productivity-irradiance curves. Heterotrophic bacterial production was determined using tritiated thymidine uptake within 24 hours of collection. We incubated each of the duplicate samplings per site with 3 replicates and one blank of 10 ml for each sample. The blank was killed with BFF to a final concentration of 4%. Stock tritiated thymidine was made with 2.58 mCi radioisotope with 30 ml of filter sterilized (0.2 um) water from Shark River. Each replicate and blank received 1.72 uCi ml-1 and was incubated at 22 degrees C for one hour. We stopped the incubation period with TCA to final concentration of 5% then put the samples on ice for 15 to 45 minutes before filtering the sample onto nitrocellulose filters. A series of 3 rinses with 5% TCA and 80% ETOH of 1 ml each and filters were placed in scintillation vials. We added liquid scintillation cocktail and counted the samples after 24 hours with a Beckman liquid scintillation counter (Model 3801).

    Instrumentation
    Zeiss microscope autoclave laboratory balance PAM desktop model fluorometer Gilford V Spectrofluorometer Beckman liquid scintillation counter (Model 3801) Hydrolab Probe Meter 4A Mini Sonde datasonde

    Quality Control
    SERC QAQC for nutrient data, blank controls and isotope blanks for tritiated thymidine uptake incubations per sample, standard curves were generated for each APA analysis, negative controls for bacteria counts using DAPI epifluorescence techniques.
  • Distribution and Intellectual Rights
    Online distribution
    http://fcelter.fiu.edu/perl/public_data_download.pl?datasetid=LT_ND_Dailey_002.txt
    Data Submission Date:  2003-02-25

    Intellectual Rights
    These data are classified as 'Type I' whereby original FCE LTER experimental data collected by individual FCE researchers are available for scientific purposes 2 years after collection. Maximum lock period = 2 years. All publications based on this dataset must cite the data Contributor, the Florida Coastal Everglades Long-Term Ecological Research (LTER) Program and that this material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DEB-1237517, #DBI-0620409, and #DEB-9910514. Additionally, two copies of the manuscript must be submitted to the Florida Coastal Everglades LTER Program Office, LTER Program Manager, Florida International University, Southeast Environmental Research Center, OE 148, University Park, Miami, Florida 33199. For a complete description of the FCE LTER Data Access Policy and Data User Agreement, please go to FCE Data Management Policy at http://fcelter.fiu.edu/data/DataMgmt.pdf and LTER Network Data Access Policy at http://fcelter.fiu.edu/data/core/data_user_agreement/distribution_policy.html.

  • Keywords
    FCE, Florida Coastal Everglades LTER, ecological research, long-term monitoring, primary production, Shark River Slough, Everglades National Park, inorganic nutrients, dissolved organic carbon, water-column biogeochemistry, total nitrogen, total organic nitrogen, total organic carbon, nitrate + nitrite, nitrate, nitrite, ammonium, total phosphorus, silica, soluble reactive phosphorus, srp, turbidity, chlorophyll a, alkaline phosphatase, bacteria abundance, dissolved oxygen, salinity, water, productivity, bacteria
  • Data Table and Format
    Data Table:  Overnight Shark River Surveys from Shark River Slough, Everglades National Park (FCE), South Florida

    Entity Name:
    LT_ND_Dailey_002
    Entity Description:
    Overnight Shark River Surveys from Shark River Slough, Everglades National Park (FCE), South Florida
    Object Name:
    LT_ND_Dailey_002
    Number of Header Lines:
    1
    Attribute Orientation:
    column
    Field Delimiter:
    ,
    Number of Records:
    41